Multifactorial models to assess responses to sorghum proportion, molasses and bacterial inoculant on in vitro quality of sorghum–soybean silages

Lima, Ricardo Bezerra Torres; Díaz, R.F.; Castro, Alessandra Marnie Martins Gomes de; Hoedtke, Sandra; Fievez, Veerle

doi:10.1016/j.anifeedsci.2011.01.008

Cited by 14 publications

(27 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fermentation process mainly depends on the availability of microorganisms in the silage, the water content of the crop and the type of substrate available for fermentation [51]. In our study, the pH and WSC decreased on both treated and untreated grasses and this result agrees with previous studies by [34]. The fast decline in WSC content and high production of LA in the first 5 days of ensiling could indicate by the ability of LAB to inhabit the other microorganisms.…”

Section: Discussionsupporting

confidence: 91%

“…The silos were opened according to the scheduled time and a sub-sample of silages DM, pH, WSC, NDF and ADF were measured with the same methods used in fresh grasses. Ammonia-N was determined by procedures described by [34]. The organic acids were measured in High-performance liquid chromatography )HPLC) 1260 (Agilent Technologies, Inc., Hewlett-Packard-Strasse 8 76337 Waldbronn, Germany; column: Carbomix ® H-NP5, Sepax Technologies, Inc., Newark, DE, USA; detector: refractive indexdetector, Agilent Technologies, Inc Hewlett-Packard-Strasse 8 76337 Waldbronn, Germany; eluent: 0.5 ml/min, 2.5 mmol/L H 2 SO 4 ; temperature: 55 • C).…”

Section: Chemical and Microbial Analysis Of Fresh Grasses And Silagementioning

confidence: 99%

See 1 more Smart Citation

Fermentation Characteristics of Lactobacillus Plantarum and Pediococcus Species Isolated from Sweet Sorghum Silage and Their Application as Silage Inoculants

et al. 2019

View full text Add to dashboard Cite

This study aims to evaluate the fermentation characteristics of Lactobacillus plantarum and Pediococcus spp isolated from sweet sorghum silage to enhance the fermentation quality of Napier grass and sweet sorghum silage. Based on molecular 16S ribosomal ribonucleic identification the isolated strains were phylogenetically related to Lactobacillus plantarum (HY1), Pediococcus acidilactici (HY2) and Pediococcus claussenii (HY3). Strains HY1, HY2 and HY3 and commercial bacteria Lactobacillus plantarum, Ecosyl; (MTD\1( were ensiled with sweet sorghum and Napier grass and the non-inoculated grasses, have been arranged in a completely randomized experimental design in a 5 (inoculants) × 3 (ensiling periods). In both grasses, the fermentation characteristics chemical composition and microbial population were assessed at 5–30 and 90 days of ensiling. The results showed that the effect of addition inoculants significantly reduced (p < 0.05) the pH, ammonia-N, acetic acid and undesirable microbial population and increased (p < 0.05) lactic acid and lactic acid bacteria counting when compared to the control. The effect of ensiling days on silage quality through the increasing lactic acid, acetic acid, ammonia-N, propionic acid and butyric acid whereas decreasing pH and water-soluble carbohydrates and microbial counts. In both sweet sorghum and Napier silage treated with isolated strains showed the best results in silage quality. The HY3 belongs to Pediococcus claussenii was not extensively studied in silage but it has shown good fermentation quality which strongly recommended to apply as probiotic.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Chemical and Microbial Analysis Of Fresh Grasses And Silagementioning

confidence: 99%

Fermentation Characteristics of Lactobacillus Plantarum and Pediococcus Species Isolated from Sweet Sorghum Silage and Their Application as Silage Inoculants

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Finally, in all silages the acetic acid/total fermentation acids ratio was found to be >0.57 which is considerable higher than the recommended value, i.e. <0.20 (Lima et al, 2011). Therefore, it may be speculated that the aerobic stability (Weinberg et al, 1993;Kung and Ranjit, 2001;Danner et al, 2003) of all silages can be disputed.…”

Section: Fermentation Qualitymentioning

confidence: 74%

“…The relative high proportions of acetic acid are difficult to explain but they may be related to a relative lack of rapid fermentable carbohydrates in OPF. Indeed, the use of appropriate amounts of molasses as an additive has been shown to produce silages with low proportions of acetic acid in combination with high proportions of lactic acid (Bureenok et al, 2012;Lima et al, 2011).…”

Section: Fermentation Qualitymentioning

confidence: 99%

The Effects of Adding Lactic Acid Bacteria and Cellulase in Oil Palm(Elais GuineensisJacq.) Frond Silages on Fermentation Quality, Chemical Composition and in Vitro Digestibility

Ebrahimi

Rajion

Goh

et al. 2014

Italian Journal of Animal Science

View full text Add to dashboard Cite

The main objective of the current study was to evaluate whether oil palm frond (OPF) can be successfully ensiled without or with the additives cellulase or lactic acid bacteria (LAB). Thus, fresh OPF was ensiled either without additives or with cellulase or LAB or their combination. Ensiling was carried out by storing 2 kg samples in airtight glass jars at 25-30°C for 12 weeks. Thereafter, the silage samples were subjected to proximate analyses, an in vitro digestibility assay and measures on selected indices of fermentation. Fermentation of OPF without additives appeared to be unsuccessful as both pH and ammonia content were too high (4.9 and 9.9%, respectively). In contrast, the use of cellulase or LAB resulted in silages with a pH<4.5 and ammonia fractions <8.4%, but the lowest values were found when both cellulase and LAB were used, i.e. pH=4.1 and ammonia fraction=6.7%. In vitro digestibility of dry matter was significantly higher in the cellulase treated silages. The process of ensiling was associated with both a significant decrease of the fat content of OPF and a significant change of the fatty acid profile. However, the proportions of major fatty acids (C16:0 and C18:2n-6) were not affected by the process of ensiling. In conclusion, the use of cellulase additive appears a practical tool to safeguard the process of fermentation. Using a cellulase enzyme or its combination with LAB improves the fermentation profile and increases the nutritional value of the OPF silage.

show abstract

“…Silage dry matter was corrected considering SCFA, lactic acid, alcohol and ammonia losses according to Lima et al . (2011 b ), depending on silage pH.…”

Section: Methodsmentioning

confidence: 99%

Combined conservation of jack bean and velvet bean with sorghum: evaluation of lab-scale silages and in vitro assessment of their nutritive value

et al. 2014

Self Cite

View full text Add to dashboard Cite

The potential of ensiled sorghum-soybean as a ruminant feed has already been demonstrated; however, alternatives for soybean should be considered because other legumes such as jack bean (JB; Canavalia ensiformis (L.) DC.) and velvet bean (VB; Mucuna pruriens (L.) DC) might produce better yields under tropical conditions. First, the possibility for a qualitative conservation of these legumes in combination with sorghum was studied using lab-scale silages. Furthermore, the potential of additional molasses as a source of water-soluble carbohydrates (WSC) and a microbial inoculant (BIOPRANAL) to improve silage quality were assessed. As sorghum or legume tannins may influence the silage nutritive value, their importance was assessed through the addition of polyethylene glycol (PEG), which reduces tannin activity. Therefore, 25 treatments per legume-sorghum combination were created according to a central composite design. An acceptable silage quality was reached when at least half of the fresh biomass consisted of sorghum in combination with at least 15 g of additional WSC/kg fresh material. The nutritive value of mixed silages as well as pure sorghum silage in combination with dry jack beans was determined through in vitro digestibility in the rumen and small intestine. Polyethylene glycol increased the in vitro production of short-chain fatty acids, ammonia (NH3) concentrations and effective rumen dry matter and crude protein degradability for both mixed silages, indicating that tannins reduced rumen degradability. The latter action could be positive as the amount of digestible bypass protein increased when no PEG was added in the silo. Propionate and valerate proportions were increased through PEG addition to sorghum-JB and sorghum-VB silages, respectively, suggesting that the nature of the tannins differ between these legumes

show abstract

Multifactorial models to assess responses to sorghum proportion, molasses and bacterial inoculant on in vitro quality of sorghum–soybean silages

Cited by 14 publications

References 15 publications

Fermentation Characteristics of Lactobacillus Plantarum and Pediococcus Species Isolated from Sweet Sorghum Silage and Their Application as Silage Inoculants

Fermentation Characteristics of Lactobacillus Plantarum and Pediococcus Species Isolated from Sweet Sorghum Silage and Their Application as Silage Inoculants

The Effects of Adding Lactic Acid Bacteria and Cellulase in Oil Palm(Elais GuineensisJacq.) Frond Silages on Fermentation Quality, Chemical Composition and in Vitro Digestibility

Combined conservation of jack bean and velvet bean with sorghum: evaluation of lab-scale silages and in vitro assessment of their nutritive value

Contact Info

Product

Resources

About