Multifaceted benefits of Bacillus amyloliquefaciens strain FBZ24 in the management of wilt disease in tomato caused by Fusarium oxysporum f. sp. lycopersici

Elanchezhiyan, K.; Keerthana, U.; Nagendran, K.; Prabhukarthikeyan, S.R.; Prabakar, K.; Raguchander, T.; Karthikeyan, G.

doi:10.1016/j.pmpp.2018.05.008

Cited by 62 publications

(34 citation statements)

References 40 publications

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“…FZB42 and its closely related ‘cousin’ FZB24, are successfully used as biofertilizer and biocontrol bacteria in agriculture being especially efficient against fungal and bacterial pathogens 2 . Beneficial effects of FZB42/FZB24 on plant growth and disease suppression in field trials were reported for potato ( Schmiedeknecht et al, 1998 ), cotton ( Yao et al, 2006 ), strawberry ( Sylla et al, 2013 ), wheat ( Talboys et al, 2014 ), lettuce ( Chowdhury et al, 2013 ), and tomato ( Elanchezhiyan et al, 2018 ), for example.…”

Section: Introduction and Short History Of Gram-positive Pgpr Researcmentioning

confidence: 95%

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

et al. 2018

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Bacillus velezensis FZB42, the model strain for Gram-positive plant-growth-promoting and biocontrol rhizobacteria, has been isolated in 1998 and sequenced in 2007. In order to celebrate these anniversaries, we summarize here the recent knowledge about FZB42. In last 20 years, more than 140 articles devoted to FZB42 have been published. At first, research was mainly focused on antimicrobial compounds, apparently responsible for biocontrol effects against plant pathogens, recent research is increasingly directed to expression of genes involved in bacteria–plant interaction, regulatory small RNAs (sRNAs), and on modification of enzymes involved in synthesis of antimicrobial compounds by processes such as acetylation and malonylation. Till now, 13 gene clusters involved in non-ribosomal and ribosomal synthesis of secondary metabolites with putative antimicrobial action have been identified within the genome of FZB42. These gene clusters cover around 10% of the whole genome. Antimicrobial compounds suppress not only growth of plant pathogenic bacteria and fungi, but could also stimulate induced systemic resistance (ISR) in plants. It has been found that besides secondary metabolites also volatile organic compounds are involved in the biocontrol effect exerted by FZB42 under biotic (plant pathogens) and abiotic stress conditions. In order to facilitate easy access to the genomic data, we have established an integrating data bank ‘AmyloWiki’ containing accumulated information about the genes present in FZB42, available mutant strains, and other aspects of FZB42 research, which is structured similar as the famous SubtiWiki data bank.

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Section: Introduction and Short History Of Gram-positive Pgpr Researcmentioning

confidence: 95%

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

et al. 2018

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“…As a Bacillus sp. strain, B. velezensis has been reported to be effective in controlling F. oxysporum‐ caused wilt disease in tomato (Elanchezhiyan et al ), indicating its potential as a pest biocontrol agent on other crops. In this study, an endophytic bacterium, B. velezensis strain B‐36, was isolated from lotus plants, which showed high antifungal activity against F. oxysporum .…”

Section: Discussionmentioning

confidence: 99%

Endophytic Bacillus velezensis strain B‐36 is a potential biocontrol agent against lotus rot caused by Fusarium oxysporum

et al. 2019

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Aim The aim of this study was to screen potential lotus plant endophytic bacterial isolate for effective inhibition against lotus rot causing fungal pathogen Fusarium oxysporum. Methods and Results In this study, endophytic bacteria were isolated from lotus tissues and tested for antagonistic activities against the pathogenic fungus F. oxysporum. Among the putative endophytic Bacillus strains identified, suspensions of the strain B‐36 showed the highest inhibition rate against F. oxysporum growth. Pot assays indicated that B‐36 was effective in controlling F. oxysporum‐inducing lotus rot. However, the control efficiency varied with the inoculation method and concentration, where injection of 800 μl B‐36 suspension per plant (2 × 108 CFU per ml) into stems showed the highest control efficiencies of 77·1 and 60·0% for pre‐inoculation and post‐inoculation. In addition, the colonizing population levels (CPLs) of B‐36 on lotus also varied with the inoculation method and concentration, with the highest CPLs, that is, 3·05 and 2·83 log(CFU per gram), being observed on lotus leaves and stems respectively for stem injection of 200 μl per plant. Moreover B‐36 showed no noticeable effects on lotus seed germination rate or seedling growth. Finally, B‐36 was characterized as Bacillus velezensis based on its morphology, Gram‐positive characteristics, as well as its 16S rDNA and gyrB sequences. Conclusion The isolate B‐36 can be applied as a biocontrol agent against F. oxysporum‐inducing lotus rot. Significance of Impact of the Study The soil‐borne fungus F. oxysporum causes lotus rot and severe yield loss, and currently available control methods are very limited. Here we identify a new promising biocontrol agent against lotus rot caused by F. oxysporum.

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“…In field studies, we provide a complete set of techniques for the application of PGPRs in field control of TBS including how to prepare bioagents and how to use them in field. The combination application method similar to K. Elanchezhiyan et al(79) was adopted to evaluate the TBS suppression activity of strain Ba168 and its rivals. Results show that the disease control efficacy of strain Ba168 is lower than MPA in two tobacco varieties, but higher than Dimethomorph preparations.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

Guo

Yuan

Luo

et al. 2019

Preprint

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Abstract： 9Tobacco black shank (TBS) caused by Phytophthora nicotianae is destructive to almost all 10 kinds of tobacco cultivars and is widespread in many tobacco-planted countries. Here, an isolated 11 plant growth-promoting rhizobacteria (PGPR) strain Ba168 is promise in biocontrol of TBS. In 12 vitro assays disclosed a strong P. nicotianae suppression activity and the field utilization potential 13 (FUP) by characterized the crude extract of culture filtrates of Ba168. P. nicotianae's growth was 14 inhibited by the crude extract at minimum inhibitory concentration (MIC) of 5μl/mL. Extracellular 15 conductivity, pH and the wet, dry weight of P. nicotianae's mycelia, were significantly different 16 after treated with different concentrations of the crude extract and the deformity and perforation of 17 treated P. nicotianae's hyphae can be observed in scanning electron microscope (SEM) analysis. 18Proteome characterizations of the crude extract were used as supplementary proofs that further 19 evaluated FUP of Ba168. We then identified strain Ba168 as B. amyloliquefaciens by its genetic 20 and phenotypic characteristics. Field assays comparatively evaluated TBS control efficacy of these 21PGPRs and agrochemicals. Pooling analysis of the results showed that the biocontrol efficacy of 22Ba168 preparation is only lower than Mixture of Propamocarb hydrochloride and Azoxystrobin 23 (MPA) but better than other tested subjects. Although the existence of differences in biocontrol 24 efficacy, PGPR preparations effectively reduced the disease index of tobacco. 25

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Multifaceted benefits of Bacillus amyloliquefaciens strain FBZ24 in the management of wilt disease in tomato caused by Fusarium oxysporum f. sp. lycopersici

Cited by 62 publications

References 40 publications

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

Endophytic Bacillus velezensis strain B‐36 is a potential biocontrol agent against lotus rot caused by Fusarium oxysporum

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

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