Multienzyme chemiluminescent foldable biosensor for on-site detection of acetylcholinesterase inhibitors

Montali, Laura; Calabretta, Maria Maddalena; Lopreside, Antonia; D’Elia, Marcello; Guardigli, Massimo; Michelini, Elisa

doi:10.1016/j.bios.2020.112232

Cited by 79 publications

(51 citation statements)

References 52 publications

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“…Both the selected CCD camera and the CMOS-smartphone sensors previously demonstrated their suitability as portable light sensors for BL and CL assays. 23 , 24 The OnePlus smartphone-integrated CMOS was reported to be the best performing smartphone-integrated sensor by Kim et al, who performed a comparison with five different types of smartphones and reported that best results were achieved by OnePlus One. 34 Figure 2 shows concentration–response curves obtained with the three portable light detectors (ATIK 383L+, OnePlus 6 smartphone, and SiPM sensor).…”

Section: Resultsmentioning

confidence: 99%

“…The suitability of LuminoSiPM for the detection of CL signals was also evaluated. An origami sensing paper based on the inhibition of AChE activity by molecules such as OP pesticides and nerve agents 23 was optimized and adapted to fit the LuminoSiPM device ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…An origami sensing paper previously described 23 was modified to fit the LuminoSiPM and the analytical procedure adapted. The sensing paper consisted of four circular hydrophilic “wells” with a diameter of 5 mm, surrounded by hydrophobic areas obtained by wax printing.…”

Section: Methodsmentioning

confidence: 99%

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Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device

et al. 2021

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The availability of portable analytical devices for on-site monitoring and rapid detection of analytes of forensic, environmental, and clinical interest is vital. We report the development of a portable device for the detection of biochemiluminescence relying on silicon photomultiplier (SiPM) technology, called LuminoSiPM, which includes a 3D printed sample holder that can be adapted for both liquid samples and paper-based biosensing. We performed a comparison of analytical performance in terms of detectability with a benchtop luminometer, a portable cooled charge-coupled device (CCD sensor), and smartphone-integrated complementary metal oxide semiconductor (CMOS) sensors. As model systems, we used two luciferase/luciferin systems emitting at different wavelengths using purified protein solutions: the green-emitting P. pyralis mutant Ppy-GR-TS (λ max 550 nm) and the blue-emitting NanoLuc (λ max 460 nm). A limit of detection of 9 femtomoles was obtained for NanoLuc luciferase, about 2 and 3 orders of magnitude lower than that obtained with the portable CCD camera and with the smartphone, respectively. A proof-of-principle forensic application of LuminoSiPM is provided, exploiting an origami chemiluminescent paper-based sensor for acetylcholinesterase inhibitors, showing high potential for this portable low-cost device for on-site applications with adequate sensitivity for detecting low light intensities in critical fields.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device

et al. 2021

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“…The developed 3D origami-based CL immunodevice was combined with a luminol-H 2 O 2 CL system catalyzed by Ag nanoparticles and it was demonstrated that the excellent analytical performances allowed the simultaneous detection of four tumor markers. The same origami-based approach exploiting wax printing technology was recently proposed for the development of a multienzyme CL foldable paper-based biosensor for on-site detection of acetylcholinesterase (AChE) inhibitors [ 63 ].…”

Section: Fluid Control and Fluid Handlingmentioning

confidence: 99%

Paper-Based Immunosensors with Bio-Chemiluminescence Detection

Calabretta

Zangheri

Calabria

et al. 2021

Sensors

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Since the introduction of paper-based analytical devices as potential diagnostic platforms a few decades ago, huge efforts have been made in this field to develop systems suitable for meeting the requirements for the point-of-care (POC) approach. Considerable progress has been achieved in the adaptation of existing analysis methods to a paper-based format, especially considering the chemiluminescent (CL)-immunoassays-based techniques. The implementation of biospecific assays with CL detection and paper-based technology represents an ideal solution for the development of portable analytical devices for on-site applications, since the peculiarities of these features create a unique combination for fitting the POC purposes. Despite this, the scientific production is not paralleled by the diffusion of such devices into everyday life. This review aims to highlight the open issues that are responsible for this discrepancy and to find the aspects that require a focused and targeted research to make these methods really applicable in routine analysis.

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“…Several examples have been listed since the pioneering studies of Leland C. Clark, Jr in 1956, Leland Clark in 1962 and urea biosensors by Guilbault and Montalvo, Jr, in 1969. [ 90–93 ]…”

Section: Cell‐free Bioluminescence Biosensorsmentioning

confidence: 99%

Bioluminescence goes portable: recent advances in whole‐cell and cell‐free bioluminescence biosensors

et al. 2020

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Recent advancements in synthetic biology, organic chemistry, and computational models have allowed the application of bioluminescence in several fields, ranging from well established methods for detecting microbial contamination to in vivo imaging to track cancer and stem cells, from cell‐based assays to optogenetics. Moreover, thanks to recent technological progress in miniaturized and sensitive light detectors, such as photodiodes and imaging sensors, it is possible to implement laboratory‐based assays, such as cell‐based and enzymatic assays, into portable analytical devices for point‐of‐care and on‐site applications. This review highlights some recent advances in the development of whole‐cell and cell‐free bioluminescence biosensors with a glance on current challenges and different strategies that have been used to turn bioassays into biosensors with the required analytical performance. Critical issues and unsolved technical problems are also highlighted, to give the reader a taste of this fascinating and challenging field.

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Multienzyme chemiluminescent foldable biosensor for on-site detection of acetylcholinesterase inhibitors

Cited by 79 publications

References 52 publications

Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device

Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device

Paper-Based Immunosensors with Bio-Chemiluminescence Detection

Bioluminescence goes portable: recent advances in whole‐cell and cell‐free bioluminescence biosensors

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