Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis identified by multiplex PCR from animals

Sj, Yang; Park, KY; Seo, Kwang-Suk; Te, Besser; Yoo, HS; Km, Noh; Sh, Kim; Bk, Lee; Yh, Kook; Yh, Park

doi:10.4142/jvs.2001.2.3.181

Cited by 17 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that storage condition and duration will not aVect the prevalence of diVerent types of bacteria on egg shells or in egg contents but the rate of multiplication and the microbial load (Humphrey et al, 1991;James et al, 2002;Schoeni et al, 1995). It has been established that resistance of bacteria to antimicrobial agents is genetically controlled (plasmids or chromosomes) (Shears, Suliman, & Hart, 1988;Washington, 1979;Yang, Park, & Seo, 2001).…”

Section: Discussionmentioning

confidence: 99%

Frequency and antimicrobial resistance of enteric bacteria with spoilage potential isolated from table eggs

Adesiyun

Offiah

Seepersadsingh

et al. 2006

Food Research International

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Frequency and antimicrobial resistance of enteric bacteria with spoilage potential isolated from table eggs

Adesiyun

Offiah

Seepersadsingh

et al. 2006

Food Research International

View full text Add to dashboard Cite

“…Four pairs of primers were used for the amplification of four individual target genes simultaneously as described by Yang et al (2001). Specific primers for sul1, sul2 and sul3 were also used to detect sulphonamide resistance genes, as described by Grape et al (2003).…”

Section: Pcrmentioning

confidence: 99%

Characterization of antimicrobial resistant Salmonella enterica serovars Enteritidis and Typhimurium isolates from animal and food in Southern Italy

et al. 2010

View full text Add to dashboard Cite

In the last two decades, the emergence and spread of antimicrobial-resistant pathogens, among them Salmonella, has become a serious health hazard worldwide, and specifically the high incidence of multidrug resistance has been encountered widely in many European countries. This study examines the antimicrobial susceptibility of Salmonella enterica strains Typhimurium and Enteritidis isolated in Campania and Calabria region (Southern Italy) from animal and food of animal origin. The relationship of antibiotic resistance phenotype and the presence of some resistance genes has been also investigated. As expected, our results show that resistance to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracycline is common, although resistance to other antibiotics (i.e.: nalidixic acid) and other resistance patterns occur. The genetic resistant patterns have been partially described for this food-borne pathogen but efforts are needed to realize the complete characterization of antimicrobial resistance genes.

show abstract

“…Several assays targeting different genes are available to specifically detect S. Enteritidis and S. Typhimurium (Soumet et al. 1999a,b; Yang et al. 2001; Pan and Liu 2002; Alvarez et al.…”

Section: Introductionmentioning

confidence: 99%

“…Chiu and Ou 1996;Kwang et al 1996;Hoorfar et al 2000). Several assays targeting different genes are available to specifically detect S. Enteritidis and S. Typhimurium (Soumet et al 1999a,b;Yang et al 2001;Pan and Liu 2002;Alvarez et al 2004), but apart from S. Typhi, very few reports have been published on specific detection of other serovariants (Beuzó n et al 1997;Desai et al 2005). Thus, in spite of its increasing importance, no PCR method capable of specific demonstration or identification of S. Infantis was published in the English-language literature to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Novel PCR assay for identification of Salmonella enterica serovar Infantis

Kardos

Farkas²,

Antal³

et al. 2007

Lett Appl Microbiol

View full text Add to dashboard Cite

Aims: We developed, optimized and tested two novel PCR assays specific for Salmonella enterica subspecies enterica serovar Infantis. Methods and Results: The fljB gene was chosen as the target sequence. Primers were designed on a consensus sequence built by sequencing the fljB gene of five genetically unrelated Hungarian S. Infantis strains and using sequence data from the GenBank (http://www.ncbi.nih.gov). Two alternative assays were designed, which share the reverse primer. Both proved to be highly specific to S. Infantis, neither reacted with 42 other nontyphoidal serovariants tested. The detection limit of the assays was determined to be 105 CFU ml−1 from pure culture, and 106 CFU g−1 from artificially spiked chicken faeces samples. Conclusions: Although the detection limit is rather high to allow for using them for direct detection, the assays may be useful in identification of S. Infantis both for diagnostic and for research purposes. Significance and Impact of the Study: The described PCR assays allow for the correct identification of S. Infantis even when traditional serotyping methods fail because lack of expression of flagellar antigens.

show abstract

Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis identified by multiplex PCR from animals

Cited by 17 publications

References 0 publications

Frequency and antimicrobial resistance of enteric bacteria with spoilage potential isolated from table eggs

Frequency and antimicrobial resistance of enteric bacteria with spoilage potential isolated from table eggs

Characterization of antimicrobial resistant Salmonella enterica serovars Enteritidis and Typhimurium isolates from animal and food in Southern Italy

Novel PCR assay for identification of Salmonella enterica serovar Infantis

Contact Info

Product

Resources

About