Frequency and antimicrobial resistance of enteric bacteria with spoilage potential isolated from table eggs

Adesiyun, Abiodun A.; Offiah, Nkechi V.; Seepersadsingh, Nadira; Rodrigo, Shelly; Lashley, Victoria; Musai, L.

doi:10.1016/j.foodres.2005.07.008

Cited by 33 publications

(28 citation statements)

References 37 publications

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“…Enterobacteria in egg components is generally more important than those recovered on the loaded shell with or without feces, blood, or cracks, but the differences are not significant [10]. The microbial load of egg components is generally significant when related to the cage type of laying hens [11] as well as the source of food, the use of drugs, and exposure to high temperatures.…”

Section: Resultsmentioning

confidence: 99%

Minor Salmonella: potential pathogens in eggs in Algeria

Ayachi

Bennoune

Heleili

et al. 2015

J Infect Dev Ctries

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Introduction: Salmonellosis is one of the major foodborne diseases known to be closely related to the consumption of contaminated eggs, infected poultry, and poultry products. Control and survey of the poultry chain are the key elements and the most critical steps in the prevention of human transmission of Salmonella. Methodology: This study was carried out in East Algeria on 150 eggs meant for consumption collected from mini-markets and immediately tested for Salmonella using standard methods (ISO AFNOR 6579 modified in 2002). Briefly, the shell surfaces were carefully wiped using sterile appropriated tissues while the white and yellow yolks were separated. All 10 samples were pooled together and a total of 45 samples were carefully analyzed. Results: A contamination rate of 4.4% was found, and two strains of Salmonella bradford were isolated from white and yellow yolks. The results showed that XLT4 was the best medium for Salmonella isolation from yolks. Screening for other Salmonella in parental chickens using an enzyme-linked immunosorbent assay (ELISA) test revealed seropositive cases of Salmonella enteritidis at the top of the poultry production pyramid. Conclusions: Occurrence of Salmonella in yolks and seropositive results for S. in parental chickens is a serious and potential danger to public health. Radical and preventive measures must be taken at the critical points to control and to avoid human transmission. These measures must be installed at all levels of egg production through the application of appropriate and strict regulations, and use of good hygienic practices in transport, storage, and food preparation.

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Section: Resultsmentioning

confidence: 99%

Minor Salmonella: potential pathogens in eggs in Algeria

Ayachi

Bennoune

Heleili

et al. 2015

J Infect Dev Ctries

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“…The presence of biogenic amines in egg yolks and albumens may be associated with the presence of bacteria in the egg. Several microorganisms are producers of biogenic amines, especially Escherichia, Enterobacter, Salmonella, Shigella, and Proteus as well as Pseudomonas and Streptococcus (Lima and Gloria, 1999;Adesiyun et al, 2006;Jones and Musgrove, 2007). Despite the high level of contamination observed in the microbiological analyses in the present study, all samples of eggs showed low levels of amines.…”

Section: Biogenic Aminesmentioning

confidence: 47%

“…Moreover, the storage conditions and temperature are fundamental to the preservation of the microbiological quality of eggs (Keller et al, 1995;Cardoso et al, 2001;Radkowski, 2001;Jones et al, 2004;Adesiyun et al, 2006;Rêgo et al, 2014).…”

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confidence: 99%

Effects of packaging, mineral oil coating, and storage time on biogenic amine levels and internal quality of eggs

Figueiredo¹,

Assis²,

Menezes³

et al. 2014

Poultry Science

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This study was carried out with the aim of evaluating the effects of mineral oil application on eggshells and the use of plastic packages with lids on the physical-chemical and microbiological quality and biogenic amine contents of eggs stored under refrigeration for up to 125 d. A total of 1,920 eggs from 46-wk-old Hyline W36 laying hens were randomly distributed into 4 groups soon after classification: (i) 480 eggs were stored in pulp carton tray packages; (ii) 480 eggs were stored in plastic packages with lids; (iii) 480 eggs were stored in carton packages after the application of mineral oil; and (iv) 480 eggs were stored in plastic packages with lids after the application of mineral oil. The internal quality was measured by Haugh units, by the counts of mesophilic and psychrotrophic microorganisms, by the most probable number of total and thermal-tolerant coliforms, by the counts of molds and yeasts, by the analysis of Salmonella spp. and Staphylococcus spp., and by the levels of biogenic amines in the egg yolk and albumen. The application of mineral oil to the eggshell resulted in higher Haugh unit values throughout storage, and the use of plastic packages altered the internal quality. The application of mineral oil and the use of packaging had no effects on the microbiological and biogenic amine results. Microbiological analyses showed the absence of Salmonella spp., Staphylococcus aureus, thermal-tolerant coliforms, and fungi. However, the highest counts of mesophilic (1.1 × 10(7) cfu/g) and psychrotrophic (6.7 × 10(7) cfu/g) microorganisms were recorded. The highest values of biogenic amines detected and quantified were putrescine (2.38 mg/kg) and cadaverine (7.27 mg/kg) in the egg yolk and putrescine (1.95 mg/kg), cadaverine (2.83 mg/kg), and phenylethylamine (2.57 mg/kg) in the albumen. Despite these results, the biogenic amine levels recorded were considered low and would not be harmful to consumer health.

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“…Em relação ao conteúdo interno de ovos de feiras livres, Andrade et al (2004) observaram que E. coli e Citrobacter spp., estavam presentes em apenas 1,85% das amostras analisadas, sendo menos isoladas apenas em relação à Pseudomonas (2,94%) e Enterobacter (5,51%). Adesiyun et al (2006) observaram que Klebsiella spp. (12,9%), seguido por Pseudomonas (9,7%) foram os microorganismos mais isolados de conteúdo internos de ovos vendidos em centro comerciais de Trinidad.…”

Section: Discussionunclassified

Pesquisa de Salmonella spp. em galinhas criadas em fundo de quintal (Gallus gallus domesticus) e ovos comercializados nas feiras livres na cidade de Fortaleza, Ceará

et al. 2014

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Poultry meat and byproducts are the main protein source for man. However, such foods are related to outbreaks of food-borne infections around the world, caused mainly by Salmonella spp. Therefore, the present study aimed to investigate the presence of Salmonella spp. in material collected in properties of backyard chickens (eggs, ration, cloacal swab and drag swab) and to perform a survey of members of the Enterobacteriaceae family in eggs commercialized in the main free markets of Fortaleza. Individual cloacal swabs were collected from 405 backyard chickens from 18 houses and 10 eggs were also collected for analysis of eggshell and internal content from each sampled household, totaling 180 eggs. From the free markets, 90 eggs were collected. Once sampled, the ration, cloacal swab, drag swab, shell and internal contents of eggs were incubated in Peptone Water and aliquots were placed in RappaportVassiliadis broth and Selenite-Cystine broth added Novobiocin. Following, aliquots of each broth were streaked in plates Brilliant Green agar and MacConkey agar. Suspect colonies for Salmonella spp. were submitted to biochemical identification, with the temperature and incubation time standardized in 37ºC/24h, respectively. Eggs collected from houses were broken in sterile beaker and maintained in bacteriological incubator at 37ºc/24h. After such period, aliquots collected were incubated in RappaportVassiliadis broth and Selenite-Cystine broth added Novobiocin, following the same bacteriological procedure mentioned previously for swabs. Eggs from free markets were analyzed with the same methodology as the house eggs, minus the antibiotic Novobiocin in the Selenite-Cystin broth, and with further biochemical tests used to identify the different members of the Enterobacteriaceae family. No Salmonella spp. were isolated from swab or egg samples. However, Escherichia coli, Citrobacter spp., Enterobacter spp., Proteus spp., Providencia spp., Klebsiella spp., Shigella spp., Yersinia spp. were isolated from eggs of free markets. Accordingly to the methodology used, we may suggest that backyard chickens from Fortaleza present a satisfactory sanitary status. However, free market eggs did not present adequate sanitary conditions.

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Frequency and antimicrobial resistance of enteric bacteria with spoilage potential isolated from table eggs

Cited by 33 publications

References 37 publications

Minor Salmonella: potential pathogens in eggs in Algeria

Minor Salmonella: potential pathogens in eggs in Algeria

Effects of packaging, mineral oil coating, and storage time on biogenic amine levels and internal quality of eggs

Pesquisa de Salmonella spp. em galinhas criadas em fundo de quintal (Gallus gallus domesticus) e ovos comercializados nas feiras livres na cidade de Fortaleza, Ceará

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