Multidrug-resistant pathogenic <i>Escherichia coli</i> isolated from wild birds in a veterinary hospital

Borges, Clarissa Araújo; Beraldo, L. G.; Maluta, Renato Pariz; Cardozo, Marita Vedovelli; Barboza, Kaline; Guastalli, Elisabete Aparecida Lopes; Kariyawasam, Subhashinie; DebRoy, Chitrita; Ávila, Fernando Antônio de

doi:10.1080/03079457.2016.1209298

Cited by 15 publications

(18 citation statements)

References 46 publications

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“…In our study, one AmpC/ESBL E. coli C-O9:H9-ST1287-CH11-54 (TEM, CMY) was detected in a vaginal swab of a dog. Fluoroquinolone resistant, non-AmpC, non-ESBL E. coli ST1287 categorized as APEC has been reported in a parrot pet from Brazil [49] and in a blood sample from a patient with septicemia as well as in the feces of a healthy individual [21,50]. This ST seems to be rare and, to our knowledge, AmpC/ESBL/FQR E. coli ST1287 has been isolated from a dog for the first time.…”

Section: Discussionmentioning

confidence: 78%

Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections

et al. 2020

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The aim of the present study was to characterize Enterobacterales resistant to 3rd and 4th generation cephalosporins, carbapenems and/or fluoroquinolones, isolated from dogs and cats with urogenital infections. In total, 36 strains (Escherichia coli (n = 28), Klebsiella pneumoniae (n = 3), Serratia marcescens, Raoultella ornithinolytica, Proteus mirabilis, Citrobacter portucalensis and Enterobacter cloacae (each n = 1)) were included in the present study, 28 from Austria and 8 from Serbia. Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. Escherichia (E.) coli isolates were additionally characterized by two-locus (fumC and fimH) sequence phylotyping and multi-locus sequence typing (MLST) of selected isolates. MLST of carbapenem-resistant Enterobacter cloacae isolates was also performed. Among E. coli, the most dominant phylogenetic group was B1 (27.8%), followed by C, (16.6%), A and Clade II (5.5% each), B2 and F (2.77% each). The most predominant β-lactam resistance genes were blaTEM (70%) and blaCTX-M (38.8%), blaCMY (25%). blaNDM was detected in one carbapenem-resistant Enterobacter cloacae ST114. The most common ST among selected E. coli was 744 (10.7% isolates). The pandemic clones ST131 and ST648 carrying CTX-M-15 were also detected. Remaining STs belonged to 469, 1287, 1463 and 1642. E. coli clonotyping revealed 20 CH types. Based on the presence of certain virulence genes, three isolates were categorized as ExPEC/UPEC. The most prevalent virulence factors were fimH detected in 61%, iucD and iss both in 55%, iroN in 27.8%, papC in 13.8% and sat in 8.3% isolates.

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Section: Discussionmentioning

confidence: 78%

Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections

et al. 2020

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“…Other sequence types found across multiple households were STs 69 and 963. ST69 has been frequently associated with UTIs [35,36]; whereas ST963 is rare in humans but has been detected in wild birds and companion animals (albeit in low numbers) [30,42,43]. In the two households where ESBL producing E. coli were shared by humans and their pet dog, these strains belonged to ST131 as well as ST38; which amongst ESBL producing strains is another lineage frequently associated with blood infections in humans [39].…”

Section: Downloaded Frommentioning

confidence: 99%

“…A limitation to the study is that transmission within households cannot be examined by a cross-sectional methodology. Consequently, this is often assessed through mathematical modelling in addition to observational studies [42]. Within a longitudinal study of post-acquisition carriage of ESBL-producing Enterobacteriaceae, results were used for a transmission model that calculated a 12% probability of household transmission of ESBLproducing Enterobacteriaceae from a positive person to a negative person in the home [51].…”

Section: Study Limitationsmentioning

confidence: 99%

Carriage of Extended-Spectrum-Beta-Lactamase- and AmpC Beta-Lactamase-Producing Escherichia coli Strains from Humans and Pets in the Same Households

Toombs-Ruane

Benschop

French

et al. 2020

Appl Environ Microbiol

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Extended spectrum beta-lactamase (ESBL)- or AmpC beta-lactamase (ACBL)- producing Escherichia coli are the most common cause of community-acquired multidrug resistant urinary tract infections (UTIs) in New Zealand. The carriage of antimicrobial resistant bacteria has been found in both people and pets from the same household; thus, the home environment may be a place where antimicrobial resistant bacteria are shared between humans and pets. In this study we sought to determine whether household members (pets and people), from households of human index cases with a UTI caused by an ESBL- or ACBL- producing E. coli strain, also carried an ESBL- or ACBL- producing Enterobacteriaceae strain and if so, whether it was a clonal match to the index case clinical strain. Index cases with a community-acquired UTI were recruited based on antimicrobial susceptibility testing of urine isolates. Fecal samples were collected from 18 non-index people and 36 pets across 27 households. Eleven of the 27 households screened had non-index household members (8/18 people and 5/36 animals) positive for ESBL- and/or ACBL- E. coli. Whole-genome sequence analysis of 125 E. coli isolates (including the clinical urine isolate) from these 11 households showed that within seven households the same strain of ESBL-/ACBL- producing E. coli was cultured, from both the index case and another person (5/11 households) or pet dog (2/11 households). These results suggest that transmission within the household may contribute to community spread of ESBL- or ACBL- producing E. coli. IMPORTANCE Enterobacteriaceae that produce extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamases (ACBL) are important pathogens and can cause community-acquired illnesses such as urinary tract infections (UTIs). Fecal carriage of these resistant bacteria by companion animals may pose a risk for transmission to humans. Our work evaluated the sharing of ESBL- and ACBL- producing E. coli between humans and companion animals. We found that in some households, dogs carried the same strain of ESBL producing E. coli as the household member with a UTI. This suggests that transmission events between humans and animals (or vice versa) are likely to be occurring within the home environment and therefore the community as a whole. This is significant from a health perspective when considering measures to minimize community transmission and highlights that in order to manage community spread, we need to consider interventions at the household level.

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“…The occurrence of resistant E. coli bacteria in wild birds, including multidrug resistant strains, has been demonstrated by several authors conducting research in various countries around the world, including Poland [ 11 , 12 , 13 , 14 , 15 ]. They can also be a reservoir of APEC, as well as E. coli strains pathogenic to humans, including E. coli serotype H7:O157 [ 16 , 17 ]. Due to their migratory lifestyle, free-living birds can be contributing vectors for the spread of potentially dangerous E. coli strains between ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance and Virulence Profiles of Escherichia coli Strains Isolated from Wild Birds in Poland

et al. 2021

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Wild animals are increasingly reported as carriers of antibiotic-resistant and pathogenic bacteria including Enterobacteriaceae. However, the role of free-living birds as reservoirs for potentially dangerous microbes is not yet thoroughly understood. In our work, we examined Escherichia coli strains from wild birds in Poland in relation to their antimicrobial agents susceptibility, virulence and phylogenetic affiliation. Identification of E. coli was performed using MALDI-TOF mass spectrometry. The antibiotic susceptibility of the isolates was determined by the broth microdilution method, and resistance and virulence genes were detected by PCR. E. coli bacteria were isolated from 32 of 34 samples. The strains were most often classified into phylogenetic groups B1 (50%) and A (25%). Resistance to tetracycline (50%), ciprofloxacin (46.8%), gentamicin (34.3%) and ampicillin (28.1%) was most frequently reported, and as many as 31.2% of E. coli isolates exhibited a multidrug resistance phenotype. Among resistance genes, sul2 (31.2% of isolates) and blaTEM (28.1%) were identified most frequently, while irp-2 (31.2%) and ompT (28.1%) were the most common virulence-associated genes. Five strains were included in the APEC group. The study indicates that wild birds can be carriers of potentially dangerous E. coli strains and vectors for the spread of resistant bacteria and resistance determinants in the environment.

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Multidrug-resistant pathogenic Escherichia coli isolated from wild birds in a veterinary hospital

Cited by 15 publications

References 46 publications

Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections

Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections

Carriage of Extended-Spectrum-Beta-Lactamase- and AmpC Beta-Lactamase-Producing Escherichia coli Strains from Humans and Pets in the Same Households

Antibiotic Resistance and Virulence Profiles of Escherichia coli Strains Isolated from Wild Birds in Poland

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