Multidose intramuscular allogeneic adipose stem cells decrease the severity of canine atopic dermatitis: A pilot study

Enciso, Nathaly; Amiel-Pérez, José; Pando, John; Enciso, Javier

doi:10.14202/vetworld.2019.1747-1754

Cited by 15 publications

(17 citation statements)

References 55 publications

(50 reference statements)

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“…The protocol of Enciso et al [21] was followed to isolate ASCs. In brief, the sample was subjected to mechanical and enzymatic disaggregation, then centrifuged, and the supernatant discarded.…”

Section: Asc Isolationmentioning

confidence: 99%

First report of canine morbillivirus infection of adipose tissue-derived stem cells from dogs with distemper

et al. 2022

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Background and Aim: Ribonucleic acid viruses remain latent in different cell types, including mesenchymal stem cells; however, the distemper virus remains undetected in these cells. This study aimed to determine whether adipose stem cells (ASCs) from dogs with distemper disease are infected with the canine morbillivirus (CM). Materials and Methods: Twelve dogs with the neurological phase of the disease and who were positive for CM by reverse transcription polymerase chain reaction (RT-PCR), were studied. ASCs from adipose tissue of the lesser omentum of these infected dogs were isolated and characterized. Direct fluorescence was used to detect the viral antigen in cell cultures. Flow cytometry and RT-PCR identified detectable quantities of the virus in two cultures, while electron microscopy confirmed the CM particles within ASCs. Results: This study revealed that ASCs of the omentum of dogs with distemper disease can be infected with CM, indicating their possible involvement in this virus latency and persistence. This suggests that its detection should be considered within the quality control process of stem cells intended for regenerative medicine. Conclusion: To the best of our knowledge, this is the first study that demonstrates that omentum ASCs from dogs with distemper disease can be infected with CM and may be involved in viral latency or persistence. Our study also suggests that the detection of CM should be considered within the quality control process of stem cells intended for regenerative medicine.

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Section: Asc Isolationmentioning

confidence: 99%

First report of canine morbillivirus infection of adipose tissue-derived stem cells from dogs with distemper

et al. 2022

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“…MSC are becoming increasingly used in canine species to treat a range of diseases, including orthopedic (Dias et al, 2021), digestive tract disorders (Cristóbal et al, 2021;Pérez-Merino et al, 2015), as well as diseases of the liver (Gardin et al, 2018), kidney (Lee et al, 2017), heart (Pogue et al, 2013), respiratory, skin (Enciso et al, 2019), ocular (Villatoro et al, 2015) and reproductive system. In addition, canine MSC have been extensively studied due to the interest this species holds as preclinical/clinical models for cell therapy in humans (Hoffman & Dow, 2016).…”

Section: Introductionmentioning

confidence: 99%

Stromal Vascular Fraction From Canine Adipose Tissue Comprises Mesenchymal Stromal Cell Subpopulations Characterized by Time-dependent Binding Affinity to Culture Plastic

Scattini

Pellegrini

Severi

et al. 2022

Preprint

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BACKGROUD: Mesenchymal Stromal Cells (MSC) from adipose tissue are among the most extensively investigated cells for clinical applications both in human and veterinary medicine. Their isolation is mostly carried out by collagenase digestion followed by filtration and seeding. Non-adherent cells are then removed from culture 48 hours upon seeding. Our hypothesis is that fragments eliminated by filtration or discarded after 48h might contain residual MSC that adhere later to the plastic, also after 6 days (144 hours) upon isolation. The aim of this study is to evaluate the basic features of cells that adhere to the plastic at 3 different time points after isolation in order to speculate on the possible existence of MSC subpopulations that are located in different tissue niches.METHODS: Subcutaneous adipose tissue collected from 3 dogs was minced and digested with collagenase I. Three cell populations were obtained at different time points from isolation (48h, 96h, and 144h). They were expanded until passage 3 and characterized by flow cytometry for positive (CD90, CD44, CD29) and negative (CD14, MHC2, CD45) MSC markers as well as for CD31 (endothelial cell marker), CD146 (pericyte marker), alpha-SMA (smooth muscle cell marker). At passage 3, cells were evaluated for viability (MTT assay), doubling time, and trilineage differentiation ability.RESULTS: No significant differences between the 3 subpopulations were observed. They were all characterized by the expression of MSC positive markers and by the absence of negative markers. CD31, CD146, and alpha-SMA were expressed by less than 5% of the cells in all the 3 sub-populations. No differences in differentiation ability and viability were detected. Doubling time ranged between 25 and 30 hours in all the 3 experimental groups.CONCLUSIONS: The 3 cell subpopulations were similar in terms of immunophenotype, proliferation, and differentiation potential. In this view, the procedure of sequential adhesions seems to be a useful method to efficiently improve MSC yield. Indeed, it allows for optimized cell recovery, reducing the amount of sampled tissue and shortening the time necessary to obtain an adequate number of cells for clinical applications. However, functional differences cannot be excluded, and potency assays are required in order to explore possible distinct biological attitudes.

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“…Clinical trials evaluating MSCs for cAD have yielded generally favorable results, with three out of four uncontrolled studies reporting positive clinical findings (12)(13)(14)(15). Most recently, Kaur et al conducted the first double-blind, placebo-controlled trial evaluating MSCs for cAD, in which affected dogs received adipose-derived, allogeneic MSCs via five SQ injections distributed across the body (16).…”

Section: Introductionmentioning

confidence: 99%

“…All cAD studies to date have evaluated adipose-derived MSCs, requiring donors to undergo surgical excision of fat under general anesthesia (12)(13)(14)(15)(16). In contrast, uterine tissuederived MSCs (UMSCs) can be harvested from tissues removed during routine sterilization procedures, offering a virtually unlimited source of cells without additional surgery (17).…”

Section: Introductionmentioning

confidence: 99%

The effect of uterine-derived mesenchymal stromal cells for the treatment of canine atopic dermatitis: A pilot study

Black¹,

Zacharias²,

Hughes³

et al. 2022

Front. Vet. Sci.

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Canine atopic dermatitis (cAD) is a common allergic skin condition among dogs that may respond to treatment with mesenchymal stromal cells (MSCs). The aim of this pilot study was to evaluate the safety and efficacy of allogeneic uterine tissue-derived MSCs (UMSCs) for the reduction and control of clinical signs associated with cAD. At two sites, seven client-owned dogs with cAD received two doses of approximately 3.6 x 107 UMSCs given intravenously over 30 min, on Day 0 and Day 14, with monthly clinical follow-up until Day 90 and optional owner phone interview on Day 180. Primary outcomes were pruritus and skin lesions. Pruritus was measured by the owner-assessed Pruritus Visual Analog Scale (PVAS), with treatment success defined as a 2-point reduction in PVAS score at any timepoint after treatment. Skin lesions were evaluated by two veterinarians according to the Canine Atopic Dermatitis Extent and Severity Index (CADESI-4). The secondary outcome was safety, which was evaluated via physical exam and hematology, including complete blood count (CBC), serum chemistry, and urinalysis (UA). Treatment was generally well tolerated and associated with a significant reduction in PVAS on Day 30 that was maintained through Day 180. On Day 60, five dogs (71%) achieved treatment success (at least 2-point reduction in PVAS), and three dogs (43%) had a PVAS improvement of 4-5 points. Mean CADESI-4 score was significantly improved on Day 14, Day 30, Day 60, and Day 90, with the lowest mean score observed on Day 60. Three dogs exhibited mild and transient adverse events. These findings suggest that IV-administered allogeneic UMSCs reduce and control clinical signs of cAD, with a durable benefit lasting 3–6 months.

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Multidose intramuscular allogeneic adipose stem cells decrease the severity of canine atopic dermatitis: A pilot study

Cited by 15 publications

References 55 publications

First report of canine morbillivirus infection of adipose tissue-derived stem cells from dogs with distemper

First report of canine morbillivirus infection of adipose tissue-derived stem cells from dogs with distemper

Stromal Vascular Fraction From Canine Adipose Tissue Comprises Mesenchymal Stromal Cell Subpopulations Characterized by Time-dependent Binding Affinity to Culture Plastic

The effect of uterine-derived mesenchymal stromal cells for the treatment of canine atopic dermatitis: A pilot study

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