Multidimensional Solid-State Nuclear Magnetic Resonance for Correlating Anisotropic Interactions under Magic-Angle Spinning Conditions

Determination of Multiple φ-Torsion Angles in Proteins by Selective and Extensive 13C Labeling and Two-Dimensional Solid-State NMR

“…2b). The isolated methyl carbon labels are potentially useful for measuring long-range 13 (30,31). Overall, the predicted labeling patterns are borne out well by the solution NMR spectra, as shown in Tables 1 and 2.…”

“…The high spectral sensitivity and resolution achieved with S&E 13 C labeling makes possible a variety of solid-state 2D correlation techniques for measuring structural constraints such as torsion angles (24,28,30,31,39,40) and for sequential resonance assignment (41-43). We first demonstrate the feasibility of residue-specific torsion-angle determination on such a selectively labeled protein by measuring backbone angles in 13 C2-ubiquitin.…”

Relative CSA–Dipolar Orientation from REDOR Sidebands

“…[14]). The only parameters external to the REDOR experiment are the CSA anisotropic and asymmetry values, which are relatively straightforward to determine (31,33).…”

“…[12]- [14] allow for a quicker, less tedious analysis of sideband data. From a data-processing perspective, this approach does not require a multitude of simulated spectra to analyze.…”

Compound Radiofrequency-Driven Recoupling Pulse Sequences for Efficient Magnetization Transfer by Homonuclear Dipolar Interaction under Magic-Angle Spinning Conditions

“…An FID with 1024 data points was zero-filled to 4096 points and Fourier-transformed with an exponential linebroadening factor of 100 Hz. The recycle delay was 7 s. All the experiments were performed at room temperature on a Chemagnetics CMX-400 Infinity spectrometer with a broadband double-resonance probe for a 4-mm rotor under a static magnetic field of 9.4 T. The powdered sample was composed of natural-abundance L-alanine and 97% 13 C, 15 N-labeled L-alanine purchased from Shoko Co. Ltd. with the ratio 5:1.…”