Multidimensional Flow Cytometry Immunophenotyping of Hematologic Malignancy

Pagnucco, G.; Vanelli, Laura; Gervasi, Francesco

doi:10.1111/j.1749-6632.2002.tb04123.x

Cited by 10 publications

(12 citation statements)

References 25 publications

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“…However, the increased capabilities and flexibility of high-speed sorters to measure many parameters in parallel using multiple lasers with high sensitivity and accuracy has greatly Flow Cytometry and Cell Sorting 27 benefited both researchers and clinicians alike [12][13][14][15][16][17]. This ability to extract more information per passing particle coupled with the ability to isolate desired cells at relatively high throughput make high-speed cell sorters a powerful and versatile technology.…”

Section: Clinical Diagnosticsmentioning

confidence: 99%

Flow Cytometry and Cell Sorting

Ibrahim

Engh

2007

Advances in Biochemical Engineering/Biotechnology

118

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Flow cytometry and cell sorting are well-established technologies in clinical diagnostics and biomedical research. Heterogeneous mixtures of cells are placed in suspension and passed single file across one or more laser interrogation points. Light signals emitted from the particles are collected and correlated to entities such as cell morphology, surface and intracellular protein expression, gene expression, and cellular physiology. Based on user-defined parameters, individual cells can then be diverted from the fluid stream and collected into viable, homogeneous fractions at exceptionally high speeds and a purity that approaches 100%. As such, the cell sorter becomes the launching point for numerous downstream studies. Flow cytometry is a cornerstone in clinical diagnostics, and cheaper, more versatile machines are finding their way into widespread and varied uses. In addition, advances in computing and optics have led to a new generation of flow cytometers capable of processing cells at orders of magnitudes faster than their predecessors, and with staggering degrees of complexity, making the cytometer a powerful discovery tool in biotechnology. This chapter will begin with a discussion of basic principles of flow cytometry and cell sorting, including a technical description of factors that contribute to the performance of these instruments. The remaining sections will then be divided into clinical- and research-based applications of flow cytometry and cell sorting, highlighting salient studies that illustrate the versatility of this indispensable technology.

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Section: Clinical Diagnosticsmentioning

confidence: 99%

Flow Cytometry and Cell Sorting

Ibrahim

Engh

2007

Advances in Biochemical Engineering/Biotechnology

118

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“…Contrary to the case in de novo AML, few phenotypic data have been compiled regarding MDS blasts 10 . One of the main reasons for this is that MDS blasts are not predominant cells in the BM and peripheral blood (PB), making reliable analysis of blasts difficult.…”

Section: Immunophenotypic Analysis Of Blast Cellsmentioning

confidence: 99%

“…Because morphologic evaluation is inherently subjective, and cytogenetics, while objective, identifies abnormalities in only 30–40% of cases of MDS, additional objective correlates of MDSs are needed. Flow cytometric immunophenotyping represents a highly sensitive and reproducible method for quantitative and qualitative evaluation of hematopoietic cells in the different maturative compartments during normal myelopoiesis, 10 and several groups have used flow cytometry (FC) in the study of MDS. MDS patients have been found to have abnormal expression of several single‐surface antigens, as indicated by either the intensity of fluorescence or the percentage of positive cells 11,12 .…”

Section: Introductionmentioning

confidence: 99%

The Role of Flow Cytometric Immunophenotyping in Myelodysplastic Syndromes

Pagnucco¹,

Giambanco²,

Gervasi³

2006

Annals of the New York Academy of Sciences

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The myelodysplastic syndromes (MDSs) are a group of heterogeneous hematological disorders characterized by bilineage or trilineage dysplastic morphology, abnormal clonal populations, progressive bone marrow failure, and a high rate of transformation to acute myeloid leukemia. A combination of morphology, to detect multilineage dysplasia in the bone marrow and peripheral blood, and cytogenetics to detect characteristic clonal abnormalities, is used in establishing a diagnosis of MDS. Although diagnostic criteria are well established, a significant number of patients have blood and bone marrow findings that make diagnosis and classification difficult. Flow cytometric immunophenotyping is an accurate and highly sensitive method for quantitative and qualitative evaluation of hematopoietic cells in the different maturative compartments, and several groups have used flow cytometry in the study of MDSs. Findings of recent studies suggest that flow cytometry immunophenotyping might provide useful information in the diagnosis and the management of MDS patients.

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“…The traditional side scatter and forward scatter SSC/FSC gating is still widely used. However, gating by CD45/SSC is more accurate [4]. Other options are sequential and back gating.…”

Section: Methodsmentioning

confidence: 99%

Flow cytometry in acute leukemia

Saxena

Anand

2008

Indian J Hematol Blood Transfus

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Immunophenotyping of acute leukemia is one of the most important clinical application of Flow cytometery. The aim of this work is to review recent advances in fl ow cytometery methods, quality control, troubleshooting and its prevention and data analysis of acute leukemia. Multiparameter fl ow cytometery is a useful adjunct to morphology and cytochemistry and it is an invaluable tool in the diagnosis of acute leukemia.

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Multidimensional Flow Cytometry Immunophenotyping of Hematologic Malignancy

Cited by 10 publications

References 25 publications

Flow Cytometry and Cell Sorting

Flow Cytometry and Cell Sorting

The Role of Flow Cytometric Immunophenotyping in Myelodysplastic Syndromes

Flow cytometry in acute leukemia

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