Multicolor Discrete Frequency Infrared Spectroscopic Imaging

Yeh, Kevin; Lee, Dongkwan; Bhargava, Rohit

doi:10.1021/acs.analchem.8b04749

Cited by 26 publications

(42 citation statements)

References 45 publications

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“…3 ). This comparison is based on standard instruments, and more discussions on high-speed versions can be found in the literature 12 , 33 . Briefly, while the spatial resolution of SRS is expectedly finer, FTIR could be more advantageous to accommodate the full spectral coverage in a single scan ( Fig.…”

Section: Mir Microscopy As a Complementary Platform To Raman For Highmentioning

confidence: 99%

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Mid-infrared metabolic imaging with vibrational probes

et al. 2020

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Understanding metabolism is indispensable to unraveling the mechanistic basis of many physiological and pathological processes. However, in situ metabolic imaging tools are still lacking. Herein we introduce a framework for mid-infrared (MIR) metabolic imaging by coupling the emerging high-information-throughput MIR microscopy with specifically designed IR-active vibrational probes. Three categories of small vibrational tags including azide bond, 13 C-edited carbonyl bond and deuterium-labeled probes are presented to interrogate various metabolic activities in cells, small organisms and mice. Two MIR imaging platforms are implemented including broadband Fourier transform infrared (FTIR) microscopy and discrete frequency infrared (DFIR) microscopy with a newly incorporated spectral region (2000–2300 cm −1 ). Our technique is uniquely suited for metabolic imaging with high-information-throughput. In particular, we performed single-cell metabolic profiling including heterogeneity characterization, and large-area metabolic imaging at tissue/organ level with rich spectral information.

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Section: Mir Microscopy As a Complementary Platform To Raman For Highmentioning

confidence: 99%

“…We note the DFIR images have some speckle patterns under the widefield configuration. This can be potentially mitigated by spatial noise dephasing 21 , 37 or point scanning system 33 , 38 .…”

Section: Technical Demonstration Of Dfir Metabolic Imaging With Vibramentioning

confidence: 99%

Mid-infrared metabolic imaging with vibrational probes

et al. 2020

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“…Recent studies have demonstrated wide-field measures of photothermal absorption and scattering (52,53). Despite providing optical compatibility, however, these methods provide fields of view and pixel counts that are much smaller than direct absorption IR microscopy (54). More importantly, data exhibit relatively poor sensitivity compared to direct absorption IR microscopy as the beam deflection is a measure of the photothermal change in the refractive index.…”

Section: Significancementioning

confidence: 99%

All-digital histopathology by infrared-optical hybrid microscopy

Schnell

Mittal

Falahkheirkhah

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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118

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Optical microscopy for biomedical samples requires expertise in staining to visualize structure and composition. Midinfrared (mid-IR) spectroscopic imaging offers label-free molecular recording and virtual staining by probing fundamental vibrational modes of molecular components. This quantitative signal can be combined with machine learning to enable microscopy in diverse fields from cancer diagnoses to forensics. However, absorption of IR light by common optical imaging components makes mid-IR light incompatible with modern optical microscopy and almost all biomedical research and clinical workflows. Here we conceptualize an IR-optical hybrid (IR-OH) approach that sensitively measures molecular composition based on an optical microscope with wide-field interferometric detection of absorption-induced sample expansion. We demonstrate that IR-OH exceeds state-of-the-art IR microscopy in coverage (10-fold), spatial resolution (fourfold), and spectral consistency (by mitigating the effects of scattering). The combined impact of these advances allows full slide infrared absorption images of unstained breast tissue sections on a visible microscope platform. We further show that automated histopathologic segmentation and generation of computationally stained (stainless) images is possible, resolving morphological features in both color and spatial detail comparable to current pathology protocols but without stains or human interpretation. IR-OH is compatible with clinical and research pathology practice and could make for a cost-effective alternative to conventional stain-based protocols for stainless, all-digital pathology.

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“…Nanoscale IR spectroscopic mapping of polymer sample with a spatial resolution of 20 nm in the 1000–1500 and 1500–1900 cm −1 ranges was realized by scanning the sampled area in one direction . Quantum cascade lasers based DFIR spectroscopic microscope working at point scanning mode has been recently proposed for faster spectral imaging by optimizing the optical parameters and reducing the overlapped data . Supercontinuum source also promotes the design of a mid‐IR multispectral imaging system for the imaging of component distribution within a tissue, showing satisfying imaging quality and acquisition speed compared to a commercial instrument, which provides new ideas for microscale spectroscopic mapping of 2D materials.…”

Section: Spectroscopic Imagingmentioning

confidence: 99%

Microscale Spectroscopic Mapping of 2D Optical Materials

Dong

Yetisen

Köhler

et al. 2019

Advanced Optical Materials

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be used for flexible touch screens and displays, printable electronics, and thinfilm photovoltaics, [4] while FETs made of graphene can be used for highly sensitive biosensors. [5] Another 2D material MoS 2 , a direct-bandgap semiconductor, has applications in ultrasensitive photodetectors, transistors, and gas-sensing devices. [6][7][8][9] Other 2D materials have also shown potential in photovoltaic devices, memory, and light-emitting diodes (LEDs). [10,11] Broad application potential of 2D materials in optoelectronic devices, photonic devices, and optical sensors is mainly based on their unique optical performances. [12] 2D materials can be fabricated based on their layered structure. In the molecular structures of these materials, the atoms are bonded hard in the same plane, but the bond effect between two lateral layers is weak due to van der Waals forces. [13] 2D materials can be roughly categorized as semimetal like graphene, semiconductor like transition metal dichalcogenides (TMDs), and insulator like hexagonal boron nitride (hBN) from the aspect of bandgap differences. [14] Due to the experimentally practical manipulation of monolayer and multilayer 2D materials, remarkable optical performances can be realized for a wide range of optical applications. For example, the number of layers of 2D materials can strongly influence the photoluminescence performance. [15] The unconventional optical performances including adsorption and emission, light sensitivity, as well as plasmonic effects of 2D materials are closely related to their inner physical properties such as carrier mobility, density of states, and band structure based on the interaction of atoms, structural symmetry, and stacking patterns. [16,17] Through the control of layer number which could influence the bandgap value, 2D materials could react to different spectrum ranging from ultraviolet to infrared light. [18] Doping strategies are also effective to modify intrinsic 2D semiconductors and improve the response with respect to an extended range of spectrum. [19,20] The assessment of the optical properties of 2D materials is indispensable for device and sensor development. [21] Based on detailed surface mapping and spectral information, the suitability of 2D materials for optical devices or sensor applications can be thoroughly studied. [22,23] Microscopy, direct 2D mapping of materials, can provide basic spatial information, [24] while spectroscopy can offer one more dimensional spectral information which is 2D materials have unique optical properties due to their ultrathin layered structures, and are emerging as promising materials for optoelectronic devices. To characterize and evaluate these properties, diffraction-limited spectroscopic mapping, also known as microscale spectroscopic imaging, has been developed to provide abundant spatial and spectral information. The usefulness of microscale spectroscopic mapping for unique property study of 2D optical materials is addressed. Advances in both mature and growing microscale spectroscopic mapping...

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Multicolor Discrete Frequency Infrared Spectroscopic Imaging

Cited by 26 publications

References 45 publications

Mid-infrared metabolic imaging with vibrational probes

Mid-infrared metabolic imaging with vibrational probes

All-digital histopathology by infrared-optical hybrid microscopy

Microscale Spectroscopic Mapping of 2D Optical Materials

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