Multicolor colorimetric visual detection of Staphylococcus aureus based on Fe3O4–Ag–MnO2 composites nano-oxidative mimetic enzyme

Liu, Yu‐Shen; Sun, Mengyue; Qiao, Wenteng; Cong, Shuang; Zhang, Yun‐Qian; Wang, Luliang; Hu, Zhenhua; Liu, Fangjie; Wang, Dacheng; Wang, Ping; Liu, Quanwen

doi:10.1016/j.aca.2022.340654

Cited by 16 publications

(10 citation statements)

References 37 publications

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“…The obtained spectrum revealed the presence of an -NH 2 functional group at 3,454 cm −1 , attributed to the stretching vibration of O-H. Additionally, peaks at 2,358 cm −1 indicated the presence of -C≡N, while the bending vibrations of C-O were observed at 1,484 cm −1 . The presence of -C=C- bonds was confirmed by the peak at 1,634 cm −1 , and the -C-C- bonds were identified by the peak at 1,083 cm −1 ( 19 , 20 ). These spectral features are likely responsible for the multicolor reaction observed in the dual-substrate system.…”

Section: Resultsmentioning

confidence: 92%

“…The magnetic nanoparticles of Fe 3 O 4 were prepared using the previous method ( 20 ). The Zn-Mn nanomaterial was synthesized through a hydrothermal process.…”

Section: Methodsmentioning

confidence: 99%

“…These nanoprobes have the potential to accurately identify and analyze the presence of bacteria ( 17 ), thereby enhancing the efficiency and precision of the bacterial detection method ( 18 ). Dual-substrate colorimetry is a commonly used analytical method for quantitatively measuring the concentration of specific substances in a solution ( 19 , 20 ). This method is based on the chemical reaction between a substrate and the target substance, resulting in observable color changes.…”

Section: Introductionmentioning

confidence: 99%

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A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe ₃ O ₄ -Zn-Mn nanoenzyme and dual substrates

Qiao,

Wang,

Yang

et al. 2024

Microbiol Spectr

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A successful development of a dual-substrate colorimetric system for rapid and multicolorimetric semi-quantification of Vibrio parahaemolyticus (V. parahaemolyticus ) has been achieved. The enzymatic activity of Fe 3 O 4 -Zn-MnO 2 -aptamer (Fe 3 O 4 -Zn-Mn-Apt) catalysts can induce the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form oxidized TMB (TMB + with a bluish green color) and ortho-phenylenediamine (OPD) to form oxidized OPD (with yellow color), resulting in a three-color comparison due to the complementary nature of bluish green and yellow. The absorbance value of TMB + at 652 nm is used for the determination of V. parahaemolyticus . After optimizing the reaction conditions, the developed dual-substrate colorimetric method exhibits high sensitivity for V. parahaemolyticus detection (limit of detection of 1.12 cfu mL −1 ) and a linear range of 0–1 × 10 4 cfu mL −1 ( R 2 = 0.9934). Additionally, as the concentration of V. parahaemolyticus increases, the reaction solution changes from bluish green to green and then to yellow, enabling semi-quantitative detection by visual observation with a visual detection limit of 10 cfu mL −1 . Furthermore, the developed dual-substrate colorimetric method demonstrates excellent selectivity for V. parahaemolyticus detection and satisfactory recovery rates when applied to the determination of V. parahaemolyticus in food samples. IMPORTANCE The Fe 3 O 4 -Zn-Mn nanomimetic enzyme demonstrates significant importance in dual-substrate colorimetric detection for V. parahaemolyticus , owing to its enhanced sensitivity, selectivity, and rapid detection capabilities. Additionally, it offers cost-effectiveness, portability, and the potential for multiplex detection. This innovative approach holds promise for improving the monitoring and control of V. parahaemolyticus infections, thereby contributing to advancements in public health and food safety.

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Section: Resultsmentioning

confidence: 92%

“…The magnetic nanoparticles of Fe 3 O 4 were prepared using the previous method ( 20 ). The Zn-Mn nanomaterial was synthesized through a hydrothermal process.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe ₃ O ₄ -Zn-Mn nanoenzyme and dual substrates

Qiao,

Wang,

Yang

et al. 2024

Microbiol Spectr

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“…Liu et al established a immunosensor based on Fe 3 O 4 /Ag-MnO 2 nanocomposites. 66 The detection concept is similar to that above, except for the addition of a step of color rendering of 3,3′,5,5′-tetramethylbenzidine (TMB) in acid solution. Blue TMB catalyzed by oxidase turns yellow in acidic environments.…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Wang,

Yang

2024

J. Agric. Food Chem.

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Staphylococcus aureus (S. aureus) is a kind of pathogenic bacteria which can lead to food poisoning, hospital, and community infections. S. aureus and methicillin-resistant S. aureus (MRSA) have become headaches for public health worldwide. Therefore, strengthening the detection of S. aureus and MRSA is a critical step to prevent and control its spread and infection. This review summarized multiple detection methods (electrochemical, optical, and other biosensors) for sensitive and efficient detection of nonresistant and resistant S. aureus. First, we have introduced the principle and methods of detection platform for S. aureus and MRSA. We also contrasted various detection strategies. Finally, the current situation and prospect of S. aureus and MRSA detection in the future are explored in depth, and its development direction of detection methods is also predicted. In this review, we found that although biosensors have shown tremendous brilliance in the field of monitoring, they are currently in the experimental stage. It can be certain that we are very close to entering the commercialization stage. The point-of care testing available to nonprofessionals will become a new direction. We firmly believe that the monitoring system will be more perfect and stable and public life will be healthier and safer.

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“…The colorimetric method has been well-received due to its high efficiency, simplicity, low cost, easy visual recognition, and promising application in field analysis(Y. Liu et al, 2023 ; T. Wu et al, 2019 ). Many single color detection sensors without peak spectrum change emerged as times required one after another(Y.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive determination of ascorbic acid based on label-free silver triangular nanoplates

Qiao

Liu

Fan

et al. 2023

Current Research in Food Science

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Multicolor colorimetric visual detection of Staphylococcus aureus based on Fe3O4–Ag–MnO2 composites nano-oxidative mimetic enzyme

Cited by 16 publications

References 37 publications

A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe ₃ O ₄ -Zn-Mn nanoenzyme and dual substrates

A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe ₃ O ₄ -Zn-Mn nanoenzyme and dual substrates

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Rapid and sensitive determination of ascorbic acid based on label-free silver triangular nanoplates

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Multicolor colorimetric visual detection of Staphylococcus aureus based on Fe3O4–Ag–MnO2 composites nano-oxidative mimetic enzyme

Cited by 16 publications

References 37 publications

A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe 3 O 4 -Zn-Mn nanoenzyme and dual substrates

A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe 3 O 4 -Zn-Mn nanoenzyme and dual substrates

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Rapid and sensitive determination of ascorbic acid based on label-free silver triangular nanoplates

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A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe ₃ O ₄ -Zn-Mn nanoenzyme and dual substrates

A multichromatic colorimetric detection method for Vibrio parahaemolyticus based on Fe ₃ O ₄ -Zn-Mn nanoenzyme and dual substrates