Multiclass mycotoxin analysis in Silybum marianum by ultra high performance liquid chromatography–tandem mass spectrometry using a procedure based on QuEChERS and dispersive liquid–liquid microextraction

Arroyo‐Manzanares, Natalia; Garcı́a-Campaña, Ana M.; Gámiz‐Gracia, Laura

doi:10.1016/j.chroma.2013.01.072

Cited by 111 publications

(65 citation statements)

References 50 publications

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“…Arroyo-Manzanares et al 40 reported that values of matrix effect close to 100% indicate that there is no significant matrix effect, while values >100% and <100% indicate signal enhancement and signal suppression, respectively. Furthermore, the obtained matrix effects are in the range of ±20% which was considered as tolerable 41 and the AFM1 was quantified using matrix-matched calibration.…”

Section: Resultsmentioning

confidence: 99%

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Kos

Hajnal²,

Jajić³

et al. 2016

ACSi

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Presence of aflatoxin M1 (AFM1) in milk should be continuously controlled in order to protect the population from risks associated with its proven toxicity and carcinogenicity. During recent years, there has been an increase in demand for development of sensitive, accurate, simple and fast method which is reliable for detection of AFM1 at low concentrations found in milk samples. For that purpose, enzyme linked immunosorbent asssay (ELISA), high performance liquid chromatography with fluorescence detector (HPLC-FLD) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were optimized and validated in order to apply them for AFM1 analysis in naturally contaminated milk samples, and to assess the closeness of agreement between results of three different methods. The obtained validation parameters indicate that all three methods are suitable for determination of AFM1 in milk samples. The statistical analysis of variance between the methods and the obtained correlation coefficients indicate that there is a strong correlation between methods. All three methods are satisfactory in meeting the requirements for official control purposes. To the best of author's knowledge, this study represents the first report of an investigation and comparison of ELISA, HPLC-FLD and HPLC-MS/MS methods for determination of AFM1 in naturally contaminated milk samples.

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Section: Resultsmentioning

confidence: 99%

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Kos

Hajnal²,

Jajić³

et al. 2016

ACSi

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“…Based on the quick, easy, cheap, effective, rugged and safe (QuEChERS) protocol adapted for the analysis of aflatoxins, ochratoxin A, enniatin A, citrinin, fusarin C and FB1 (Frenich et al 2011;Arroyo-Manzanares et al 2013;Zhang et al 2013), the present study did not use the primary and secondary amine (PSA) as the base sorbent for removing the impurities of the sample solutions (for sample purification) since it can remove FB 1 , FB 2 and FB 3 from extracts due to ion exchange.…”

Section: Sample Preparation and Preliminary Methods Characterisationmentioning

confidence: 99%

Screening survey of co-production of fusaric acid, fusarin C, and fumonisins B1, B2 and B3 by Fusarium strains grown in maize grains

et al. 2014

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Fusarium species isolated from Belgian maize were screened for their ability to produce fusarin C, fusaric acid, fumonisins B1 (FB1), FB2 and FB3 in maize grains. First, cultivation of Fusarium species in Myro liquid medium allowed overcoming the shortage of the standard of fusarin C on the market. All Fusarium verticillioides produced much higher contents of mycotoxins in Myro compared to Fusarium graminearum or Fusarium venenatum. The optimization of the LC-MS/MS method resulted in low limits of detection and quantification for fusarin C, fusaric acid, FB1, FB2 and FB3 determination in maize grains. Its application for screening the potential toxin production ability evidenced that the concentrations of the analytes were significantly increased at various levels when F. verticillioides strains were cultivated in maize grains and reached 441 mg kg(-1) for fusaric acid, 74 mg kg(-1) for fusarin C, 1,301 mg kg(-1) for FB1, 367 mg kg(-1) for FB2 and 753 mg kg(-1) for FB3.

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“…The choice of method depends on the matrix and the mycotoxin to be analyzed. MS is spreading rapidly as a promising technique for simultaneous screening, identification, and quantitative determination of a large number of mycotoxins (Arroyo-Manzanares et al 2013). Although in fluorescence method, AFB 1 and AFG 1 need a derivatization procedure to enhance fluorescence and were done by a precolumn derivatization (PARK et al 1990) or postcolumn derivatization (Tavčar-Kalcher et al 2007;Tuinstra and Haasnoot 1983).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Aflatoxins in Pistachio Using Ultrasonically Stabilized Chloroform/Water Emulsion and HPLC

Malekpour

Bayati

2015

Food Anal. Methods

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Simultaneous determinations of aflatoxins (AFs) in pistachio at μg/kg concentrations were performed using ultrasonic chloroform/water emulsion microextraction (USAEME) and HPLC-UV measurement. A chloroform/ water emulsion was stabilized using ultrasonic agitation for the purification of AFs extracted from pistachios using methanol/water. Because the method effectively purified and concentrated AFs, the AFs could be measured directly and without further preconcentration using HPLC-UV. Preparation of AF derivatives for fluorescence measurement was not required. In the optimized procedure, the AF calibration curves were linear for all AFs with correlation coefficients that ranged from 0.9913 to 0.9994. The detection limit was between 0.3 and 1.4 μg/kg, and quantification limit was obtained between 1.1 and 3.2 μg/kg (n=3); the largest relative standard deviation was obtained <9.78 % (n=3) at 3 μg/kg, and recoveries ranged from 81.2 to 101.6 % at spiking levels of 0.4, 0.8, 2, and 4 μg/kg. Application of this method to the measurement of AFs in pistachio samples indicated that USAEME coupled with HPLC-UV can determine trace AF levels. The USAEME method is simple, sensitive, and costeffective in comparison to previous methods.

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Multiclass mycotoxin analysis in Silybum marianum by ultra high performance liquid chromatography–tandem mass spectrometry using a procedure based on QuEChERS and dispersive liquid–liquid microextraction

Cited by 111 publications

References 50 publications

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Screening survey of co-production of fusaric acid, fusarin C, and fumonisins B1, B2 and B3 by Fusarium strains grown in maize grains

Simultaneous Determination of Aflatoxins in Pistachio Using Ultrasonically Stabilized Chloroform/Water Emulsion and HPLC

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