Multicenter evaluation of tetracycline fiber therapy. III. Microbiological response

Abstract

In a multicenter study of the effects of tetracycline (TC) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by DNA probe analysis. Levels of Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas (Bacteroides) gingivalis, Prevotella intermedia (Bacteroides intermedius), and Wolinella recta were quantitatively determined in samples taken at baseline, and immediately after TC fiber removal, control fiber removal, and scaling and root planing. At untreated sites, samples were taken at baseline and 10 d later. Specificity of the DNA probe method was evaluated by testing the hybridization to 83 reference cultures. Interaction of the F. nucleatum probe with Fusobacterium periodonticum, and of the W. recta probe with Wolinella curva were the only cross-hybridizations noted. Species were detected at an average sensitivity of 2.9 x 10(4) organisms per sample. Approximately 70% of sites were initially infected with P. gingivalis and F. nucleatum; 50% with P. intermedia and E. corrodens; infections with W. recta and A. actinomycetemcomitans were less common (36% and 11% respectively). The average numbers of organisms found in the plaque samples were highest for F. nucleatum, P. gingivalis, and P. intermedia (ca. 10(6)). E. corrodens, W. recta, and A. actinomycetemcomitans occurred at 10-fold lower levels. Bacterial numbers and proportions of species in subgingival sites from the five centers did not differ appreciably. Both TC fiber therapy and scaling decreased the number of sites infected with all the monitored species. The bacterial composition at untreated sites and at sites where control fibers were placed was not significantly altered. The percentage reduction of the number of sites with detectable infection varied with each species: from 86% with W. recta to approximately 40% with P. gingivalis. Significant reduction of pocket depth and bleeding occurred at TC fiber-treated sites infected with each of the species. Significant attachment level gain occurred only at sites initially infected with P. gingivalis and treated with TC fibers.

Detección de Porphyromonas gingivalis en Pacientes Adultos con Periodontitis Crónica

“…López et al (2004) estudiaron 26 pacientes con periodontitis crónica en la ciudad de Santiago, determinó que de entre las 40 especies bacterianas analizadas, todas, excepto Prevotella intermedia, colonizaron más del 75 % de los sitios y 25 especies colonizaron más del 90 % de los sitios, entre las que se encuentran las bacterias del complejo rojo (T. forsythia y T. dentícola), y P. gingivalis como la más prevalente. Goodson et al (1991) analizaron por sonda de DNA 113 adultos con periodontitis encontrándose un 70 % de sitios infectados con P. gingivalis. Dahlén et al (1996) analizaron a 20 adultos de una zona rural en Kenya obteniendo un 70 % de P. gingivalis, en comparación con otras bacterias del complejo rojo.…”

Effect of tetracycline HCL (periodontal plus AB) on Aggregatibacter actinomycetemcomitans levels in chronic periodontitis

“…When compared to other commercially available antibiotics for use as local drug delivery agents, tetracycline provides better results in terms of clinical [15] and microbiological status [16]. However, studies on the duration of the maintenance of the improved periodontal status have been contradictory.…”

Periodontal Muco-Adhesive Formulations for the Treatment of Infectious Periodontal Diseases

“…The various antibiotics used in these systems include tetracycline, doxycycline, minocycline, metronidazole, chlorhexidine (an anti-microbial agent) and ofloxacin; all of which can be considered broad spectrum in their mode of action against periodontal bacteria, most of which are anaerobes. Goodson et al [227], Lowenguth et al [228] and Mombelli et al [229] indicated that, immediately post-treatment and at extended time points up to 12 months, tetracycline delivered from an ethylene vinyl acetate fiber reduced populations of Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Campylobacter rectus, Actinobacillus actinomycetemcomitans and Eikenella corrodens. It would appear that this delivery system was able to maintain tetracycline levels high enough to disrupt pathogen colonization of the periodontal sites.…”