Multicenter evaluation of a whole‐blood filter that saves platelets

Paunovic, Dragica; Meer, Pieter F. van der; Kjeldsen‐Kragh, Jens; Kekomäki, Riitta; Larsson, Stella; Greppi, N.; Porretti, Laura; Balint, Bela; Trkuljic, Miroljub; Nedeljkovic, Ninoslav; Simonović, Ruzica; Massaro, Anna Maria; Labanca, Luciana; Kora, Shinichi; Riggert, Joachim

doi:10.1111/j.1537-2995.2004.03350.x

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…Therefore, coating materials as well as filter (membrane) materials must be adequately designed to successfully construct whole blood filters for leukocyte removal. Only a few researchers have investigated whole blood filters for leukocyte removal 16, 17…”

Section: Introductionmentioning

confidence: 99%

“…Only a few researchers have investigated whole blood filters for leukocyte removal. 16,17 Paunovic et al investigated leukocyte reduction from whole blood using leukocyte removal filters composed of multiple layers of microporous polyurethane membranes. 16 Direct removal of leukocytes from whole blood was confirmed to show higher reduction of leukocytes as well as higher recovery of the three blood components (red blood cells, platelets, and plasma) as compared with the current technique that involves treatment of each component (red blood cells, platelets, and plasma) after separation by centrifugation to remove the leukocytes.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and performance of amphiphilic copolymers for blood cell separation

2006

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Three types of amphiphilic copolymers using n-butylmethacrylate (BMA) as a hydrophobic monomer, and each of N,N'-dimethylacrylamide (DMA), N-acryloylmorpholine (AMO), and N-vinylpyrrolidone (VP) as hydrophilic comonomers were synthesized for coating filters used to remove leukocytes. The influence of the amphiphilic property of the resulting filters, which were composed of nonwoven fabrics coated with the above copolymers, on leukocyte removal and platelet permeation through the filters from whole blood was investigated. The platelet permeation ratio through hydrophobic noncoated filters was only 0.2%, because platelets in whole blood adhered easily to the hydrophobic filter material. However, filters coated with poly(AMO-co-BMA) of high AMO content showed a much higher platelet permeation ratio (nearly 90%). Further, the filters coated with poly(DMA-co-BMA) also showed high permeation ratios of platelets (more than 78%) over a broad range of DMA content in the copolymer. On the other hand, the coated filters showed slightly a higher permeation ratio of leukocytes than did the noncoated filters, resulting from the increase in hydrophilicity of the surface of the filters. Moreover, the coating of the amphiphilic copolymers on the surface of the nonwoven fabrics may have affected the pore size of the filters, affecting the permeation ratio of leukocytes more strongly than that of platelets. The coated filters effectively improved platelet permeation through the filters, with a slight increase in the permeation ratio of leukocytes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and performance of amphiphilic copolymers for blood cell separation

2006

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“…Recent military conflicts in the Middle East challenged the notion that blood product constituents are the ideal resuscitative fluid as WFWB was associated with improved mortality compared to balanced ratios of plasma, platelets, and pRBCs [6]. Unfortunately, this practice was responsible for an increased risk of transfusion transmittable infections (TTI) [12].…”

Section: Discussionmentioning

confidence: 99%

“…To avoid undesirable elimination of platelets, a platelet-sparing LR filter (Imuflex WB-SP; Terumo Corporation, Tokyo, Japan) was developed. This filter has the capability to create blood products that meet or exceed Food and Drug Administration processing (FDA) regulations and has been validated to preserve platelets while effectively leukoreducing whole blood [5][6][7]. FDA regulations, however, do not require in vivo assessment of coagulation thus the impact of the platelet-sparing LR filter on the coagulation profile and function of the platelets within whole blood products is unclear [8].…”

Section: Introductionmentioning

confidence: 99%

In vitro Analysis of the Hemostatic Properties of Whole Blood Products Prepared with a Platelet-Sparing Leukoreduction Filter

Zielinski¹,

Stubbs²,

Sf³

et al. 2018

J Thrombo Cir

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Background: Warm fresh whole blood (WFWB) is an ideal resuscitation fluid for exsanguinating patients but there are myriad logistic and infectious issues associated with its use. Cold whole blood (CWB) may be an acceptable alternative to the reconstituted whole blood (RWB), the current standard of care. A leukoreduction filter has been developed which maintains platelet count while eliminating white blood cells but its effect on platelet function is unknown. We hypothesize that CWB will retain an acceptable functional coagulation profile after filtration and over time.

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“…In contrast to the most commonly used leukodepletion model (filtration at the end of storage of blood products -post-storage leukodepletion), filtration during blood sampling or at the beginning of storage (in-line or prestorage leukodepletion) would markedly reduce cytokine concentrations [10]. It would not only contribute to the reduced incidence of cytokine-mediated transfusion reactions but also create the conditions for reduced activation of thrombocytes in vitro, with improved therapeutic efficacy [11].…”

Section: Introductionmentioning

confidence: 99%

The effect of a leukodepletion model on the activation stage of platelets

et al. 2011

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AbstractThe preparation of thrombocyte concentrates with filtration before storage (in-line) makes it possible to avoid the presence of mononuclear cells in the concentrate and proinflammatory cytokines. Therefore, this filtration may result with decreased activation of trombocyte receptors in vitro, which may improve therapeutic efficiancy. Methods. We compared two groups, each with 30 therapeutic doses of concentrated thrombocytes. We prepared the first group using the classic model from the buffy coat and the other with concentrated thrombocyte samples filtrated during sampling, so-called in-line, with the WBC filter Imuflex (Terumo). Mononuclear cells (MNC), thrombocyte, and erythrocyte counts in the units of concentrated thrombocytes were obtained on an automatic cell counter, and we used flow cytometry to measure the expression of surface thrombocyte receptors. The results demonstrated that the trombocytes prepared with pre-storage filtration contained a very low level of mononuclear cells and markedly reduced trombocyte receptors. Conclusion. The number of MNC and expression of surface thrombocyte receptors were markedly lower in the concentrated thrombocyte units prepared with in-line filtration. The thrombocytes prepared in this way contain fewer mononuclear cells, are of higher quality, are more functional, and may produce a better therapeutic effect in vivo.

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Multicenter evaluation of a whole‐blood filter that saves platelets

Abstract: Use of the WB-SP filter makes it possible to obtain three leukoreduced blood components with only one filtration step. The WB-SP filter showed good leukoreduction performance and recovery of all blood components including PLTs.

Cited by 14 publications

References 26 publications

Synthesis and performance of amphiphilic copolymers for blood cell separation

Synthesis and performance of amphiphilic copolymers for blood cell separation

In vitro Analysis of the Hemostatic Properties of Whole Blood Products Prepared with a Platelet-Sparing Leukoreduction Filter

The effect of a leukodepletion model on the activation stage of platelets

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