The effect of a leukodepletion model on the activation stage of platelets

Abstract

<jats:title>Abstract</jats:title><jats:p>The preparation of thrombocyte concentrates with filtration before storage (in-line) makes it possible to avoid the presence of mononuclear cells in the concentrate and proinflammatory cytokines. Therefore, this filtration may result with decreased activation of trombocyte receptors in vitro, which may improve therapeutic efficiancy. Methods. We compared two groups, each with 30 therapeutic doses of concentrated thrombocytes. We prepared the first group using the classic model from the buffy coat and the other with concentrated thrombocyte samples filtrated during sampling, so-called in-line, with the WBC filter Imuflex (Terumo). Mononuclear cells (MNC), thrombocyte, and erythrocyte counts in the units of concentrated thrombocytes were obtained on an automatic cell counter, and we used flow cytometry to measure the expression of surface thrombocyte receptors. The results demonstrated that the trombocytes prepared with pre-storage filtration contained a very low level of mononuclear cells and markedly reduced trombocyte receptors. Conclusion. The number of MNC and expression of surface thrombocyte receptors were markedly lower in the concentrated thrombocyte units prepared with in-line filtration. The thrombocytes prepared in this way contain fewer mononuclear cells, are of higher quality, are more functional, and may produce a better therapeutic effect in vivo.</jats:p>

Effect of Platelet Concentrates (PCs) Leucodepletion on the Activation and Efficacy of Platelet Transfusion

“…4 Leukocytes, especially mononuclear cells in allogeneic blood products, both packed red cells and platelet concentrates, can cause various side effects, including immunomodulation (Transfusion-Related Immunomodulation), acute lung damage, Graft versus Host Disease, and virus transmission. 5 Transfusion-related immunomodulatory mediated by allogeneic mononuclear cells can be prevented by reducing leukocytes both before and after storage and autologous transfusion. The allogeneic transfusion effects caused by soluble HLA peptides can be overcome by autologous transfusion, while the allogeneic transfusion effects caused by soluble mediators produced by leucocytes can be prevented by reducing them before storage.…”

“…1,15,16 High CD62P expression is the cause of faster platelet destruction in the reticuloendothelial system. 5 Therefore, an assessment of the efficacy of platelet transfusions can be measured by calculating the CCI and platelet recovery. In addition, the in vivo hemostatic efficacy after PC transfusion can be measured by the level of CD62P expression and the formation of leukocyte-platelet aggregates.…”