Multicenter evaluation of a new automated electrochemiluminescence immunoassay for the quantification of testosterone compared to liquid chromatography tandem mass spectrometry

Prevalence of metabolic disturbances among women with polycystic ovary syndrome in different regions of Brazil

“…52 However, the former is considered a suitable alternative presenting good correlations with the latter. 53 Finally, to determine the prevalence of insulin resistance in our population, we used the HOMA-IR index values instead of the hyperinsulinemic-euglycemic clamp, which is considered the reference standard method. 54 However, the HOMA-IR model is a noninvasive method; hence, it is more accessible in clinical practice and still applied in the evaluation of insulin resistance in a variety of settings.…”

“…Fourth, our testosterone measurements were obtained by electrochemiluminescence assays rather than by liquid chromatography followed by tandem mass spectrometry, which is currently the preferred technique for measuring steroid analytes 52 . However, the former is considered a suitable alternative presenting good correlations with the latter 53 . Finally, to determine the prevalence of insulin resistance in our population, we used the HOMA‐IR index values instead of the hyperinsulinemic‐euglycemic clamp, which is considered the reference standard method 54 .…”

Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals

“… Drugs/analytes Systems Linear ranges LOD (S/N = 3) Samples Ref. Testosterone Elecsys® Testo II Roche diagnostics, Germany 0.12–15.0 μg/L – Serum [48] 25-Hydroxy-vitamin D Roche Modular E-170 4.9–214.6 nM – Serum [49] Sirolimus Abbott ARCHITECT i2000 sirolimus assay / – Whole blood [50] Thyroid hormone Cobas ECLIA kits, Roche Boeringer-Mannheim, USA / – Blood [51] Tacrolimus (TAC) Elecsys tacrolimus assay, Roche diagnostics 0.5–40 ng/mL 1 ng/mL Whole blood [52] Cyclosporine (CsA) Elecsys cyclosporine assay, Roche diagnostics 30–2000 mcg/L 6.8 mcg/L Whole blood [53] Tacrolimus (TAC) Cobas®, Roche, Tokyo, Japan 0.0–26.0 ng/mL 0.89 ng/mL Whole blood [54] Cyclosporine (CsA) Elecsys® Cyclosporine assay kits 94–1238 ng/mL CsA 16 ng/mL CsA Whole blood [55] Tacrolimus (TAC) Elecsys® Tacrolimus assay kits 2.1–17.8 ng/mL TAC 0.95 ng/mL TAC …”

“…Evaluation of the ECL immunoassay (ECLIA) established with HPLC/MS and other immunoassays is an important issue, since it can provide important information of the evaluated ECL immunoassays for laboratories. Brandhorst et al [48] reported an evaluation of new ECL testosterone immunoassay (Elecsys® Testo II) compared to HPLC/MS. The new Elecsys® Testo II assay showed an improved agreement with a validated HPLC/MS assay for testosterone, particularly regarding testosterone concentrations in samples from female patients and may be a suitable alternative for laboratories with no access to mass spectrometry techniques.…”

A novel double-layer molecularly imprinted polymer film based surface plasmon resonance for determination of testosterone in aqueous media

“…As it is an important indicator of a wide variety of pathological conditions such as male and female subfertility, prostate cancer and adrenal diseases, an accurate detection of the content of testosterone is of great importance for us. Conventional testosterone analysis performed in clinical laboratories is carried out mostly with immunoassays [21,22] and chromatography [23,24]. These methods have proven to be sensitive, but they have limitations concerning specificity.…”