A novel double-layer molecularly imprinted polymer film based surface plasmon resonance for determination of testosterone in aqueous media

Synthesis of magnetic molecularly imprinted polymers with excellent biocompatibility for the selective separation and inhibition of testosterone in prostate cancer cells

“…[27][28][29] Moreover, surface imprinting has adopted various supports such as Fe 3 O 4 magnetic nanospheres during the past years, 16,30,31 which can efficiently separate complicated matrices with an external magnet. To date, TSTO-imprinted polymers that were previously reported have been mostly prepared in organic solvents, [11][12][13] which could lead to poor recognition ability in biologic samples. The Fe 3 O 4 @TSTO-MIPs we synthesized can not only recognize TSTO specifically with high affinity in human cells, which suggests that it can be used to determine the concentration of androgens in body liquid, different cells and organs, but also inhibit the function of TSTO through binding to it and sequestrating it in the cell cytoplasm.…”

Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

“…Molecularly imprinted polymers (MIPs) for measuring the concentration of hormones and metabolites have been produced in the last decade and used for optical sensing by surface plasmon resonance (SPR) to detect progesterone [7], cholesterol [7] and testosterone [7][8][9][10]. More traditional laboratory-based methods have also been used, such as: gas chromatography (GC) for measurement of anabolic steroids [11] and extraction steroids [12]; liquid chromatography (LC) for measurement of epitestosterone [13], testosterone [13][14][15], or other steroids [16]; capillary electrophoresis (EC) linked with mass spectrometry (MS) for testosterone [17], epitestosterone [17], urinary steroid hormones [18] and estrogenic endocrine disruptors [19]; diode-array detection (DAD) of steroids [20], progesterone [21] and testosterone [21] in human urine [11,17,[20][21][22][23] or in goat milk [24].…”

“…More traditional laboratory-based methods have also been used, such as: gas chromatography (GC) for measurement of anabolic steroids [11] and extraction steroids [12]; liquid chromatography (LC) for measurement of epitestosterone [13], testosterone [13][14][15], or other steroids [16]; capillary electrophoresis (EC) linked with mass spectrometry (MS) for testosterone [17], epitestosterone [17], urinary steroid hormones [18] and estrogenic endocrine disruptors [19]; diode-array detection (DAD) of steroids [20], progesterone [21] and testosterone [21] in human urine [11,17,[20][21][22][23] or in goat milk [24]. The functional and crosslinking monomers that have been used in molecular imprinting include acrylamide [16], methacrylic acid (MAA) [7][8][9][10][11]13,15,17,20,21,[24][25][26][27][28], trifluoromethacrylic acid (TFMAA) [13], 2-hydroxyethyl methacrylate (HEMA) [9,26], ethylvinylbenzene (EVB) [7], 2-vinylpyridine (2VP) [12], [12,…”

Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

“…Molecularly imprinted polymers (MIPs) for measuring the concentration of testosterone have been produced in the last decade and used for optical sensing by surface plasmon resonance (SPR) [7][8][9][10]. Laboratory-based methods such as gas chromatography (GC) [11,12], liquid chromatography (LC) [13][14][15][16] or capillary electrophoresis (EC) linked with mass spectrometry (MS) [17][18][19] or diodearray detection (DAD) [20,21] of the testosterone in human urine [11,17,[20][21][22][23] or in goat milk [24] have also been reported.…”

“…Laboratory-based methods such as gas chromatography (GC) [11,12], liquid chromatography (LC) [13][14][15][16] or capillary electrophoresis (EC) linked with mass spectrometry (MS) [17][18][19] or diodearray detection (DAD) [20,21] of the testosterone in human urine [11,17,[20][21][22][23] or in goat milk [24] have also been reported. The functional and crosslinking monomers that are utilized in these preparations include acrylamide [15], methacrylic acid (MAA) [7][8][9][10][11]13,16,17,20,21,[24][25][26][27][28], trifluoromethacrylic acid (TFMAA) [13], 2-hydroxyethyl methacrylate (HEMA) [9,26], ethylvinylbenzene (EVB) [7], 2-vinylpyridine (2VP) [12], 4-vinylpyridine (4VP) [12,20,26], dopamine (DA) [14]; divinylbenzene (DVB) [7,13], ethylene glycol dimethacrylate (EGDMA)…”