2010
DOI: 10.1016/j.jbiotec.2010.01.012
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Multicellular tumor spheroids: An underestimated tool is catching up again

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Cited by 1,424 publications
(1,461 citation statements)
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References 156 publications
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“…As cancer spheroids are more likely to mirror the 3D cellular context and therapeutically relevant pathophysiological gradients of in vivo tumors, 20,21 we further tested if human MC could also promote the growth of colon cancer spheroids. To answer this, we developed a coculture model of HT29 spheroids and human MC embedded in an extracellular matrix (ECM).…”
Section: Resultsmentioning
confidence: 99%
“…As cancer spheroids are more likely to mirror the 3D cellular context and therapeutically relevant pathophysiological gradients of in vivo tumors, 20,21 we further tested if human MC could also promote the growth of colon cancer spheroids. To answer this, we developed a coculture model of HT29 spheroids and human MC embedded in an extracellular matrix (ECM).…”
Section: Resultsmentioning
confidence: 99%
“…These results confirm that 3D cultures can be obtained by magnetic levitation of B16F10 cells that have ingested Fe 3 O 4 -containing microspheres. The morphology of the 3D culture was significantly different from that of other scaffold-free 3D cultures, which formed a spherical body of multicellular aggregates (Kelm et al 2003;Hirschhaeuser et al 2010;Souza et al 2010). In our previous study, the same method of magnetic levitation was used for preparing a 3D culture of murine fibroblast 3T3 cells, which also resulted in a multicellular spheroid (Lee and Hur 2014).…”
Section: Magnetic Levitation Of B16f10 Cellsmentioning
confidence: 86%
“…In tumor spheroids, proliferating cells are usually observed at the periphery, chemical gradients (e.g. of oxygen, nutrients, and catabolites) are at diameters between 200 and 500 lm, and a central secondary necrosis appears typically at sizes greater than 500 lm (Hirschhaeuser et al 2010). However, Ki-67 positive proliferating cells were randomly distributed across the section of a 3D culture that had a planar shape with a thickness of less than 100 lm, in which B16F10 cells were growing exponentially but with a decreased specific growth rate.…”
Section: Discussionmentioning
confidence: 99%
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“…Three-dimensional (3D) cell culture systems eliminate all these drawbacks of 2D cell culture systems, provide a high surface area for growth and migration, which can be tuned to support other cell behaviors, such as morphology, viability, proliferation, response to stimuli, differentiation or maturation and mimic human tissue micro-environment, pathological conditions and biological mechanisms much more closely [1,2]. 3D culture systems are used in an extensive area of cell-based studies [3], including cell adhesion/migration, tumour biology [4][5][6], stem cell research [7], regenerative medicine, tissue engineering and preclinical testing in drug discovery [8][9][10][11].…”
Section: Introductionmentioning
confidence: 99%