Multicavity halloysite–amphiphilic cyclodextrin hybrids for co-delivery of natural drugs into thyroid cancer cells

Abstract

Multicavity halloysite nanotube materials were employed as simultaneous carriers for two different natural drugs, silibinin and quercetin, at 6.1% and 2.2% drug loadings, respectively. The materials were obtained by grafting functionalized amphiphilic cyclodextrin onto the HNT external surface. The new materials were characterized by FT-IR spectroscopy, SEM, thermogravimetry, turbidimetry, dynamic light scattering and z-potential techniques. The interaction of the two molecules with the carrier was studied by HPLC measurements and fluorescence spectroscopy, respectively. The release of the drugs from HNT-amphiphilic cyclodextrin, at two different pH values, was also investigated by means of UV-vis spectroscopy. Biological assays showed that the new complex exhibits anti-proliferative activity against human anaplastic thyroid cancer cell lines 8505C. Furthermore, fluorescence microscopy was used to evaluate whether the carrier was uptaken into 8505C thyroid cancer cell lines. The successful results revealed that the synthesized multicavity system is a material of suitable size to transport drugs into living cells.

Direct chemical grafted curcumin on halloysite nanotubes as dual-responsive prodrug for pharmacological applications

“…Although the large hydrodynamic radius measured for HNTCur, the actual size of the prodrug, as determined by SEM (Fig. 2), is comparable to that of other halloysite based drug carrier systems that showed internalization of the nanoparticle in the cell [56].…”

Covalently modified halloysite clay nanotubes: synthesis, properties, biological and medical applications

“…Halloysite is proven to be a biocompatible material in several recent reports on cell cultures 24 and invertebrate models. 7 The uptake and toxic effects of halloysite have been investigated using human cell lines, namely breast cancer cells, 24 thyroid cancer cells, [25][26][27] hepatic cancer cells 28,29 and epithelial adenocarcinoma cells. 24 The toxicity of HNT was tested after 48 hours of incubation with fibroblast and human breast cells.…”

Halloysite nanotubes loaded with peppermint essential oil as filler for functional biopolymer film

“…Halloysite is a biocompatible material as shown by several in vitro and in vivo studies (Bellani et al, 2016;Fakhrullina, Akhatova, Lvov, & Fakhrullin, 2015;Kryuchkova, Danilushkina, Lvov, & Fakhrullin, 2016;Massaro, Piana et al, 2015). The HNT length ranges between 0.2 and 1.5 m, while the inner and the outer diameters are ca.…”

“…10-30 nm and 40-70 nm, respectively . Halloysite possesses a positive alumina inner lumen and a negative silica outer surface allowing its selective functionalization (Arcudi et al, 2014;Cavallaro, Lazzara, & Milioto, 2012), the formation of a liquid crystalline phase (Luo et al, 2013) and the encapsulation of chemically and biologically active compounds Lvov, Shchukin, Möhwald, & Price, 2008;Lvov, Wang, Zhang, & Fakhrullin, 2016;Lvov, DeVilliers, & Fakhrullin, 2016;Shutava, Fakhrullin, & Lvov, 2014;), such as drugs (Aguzzi, Cerezo, Viseras, & Caramella, 2007;Lun, Ouyang, & Yang, 2014), natural molecules (Massaro, Piana et al, 2015), marine biocides (Wei et al, 2014), cosmetics (Suh et al, 2011), and other functional agents (Abdullayev, Price, Shchukin, & Lvov, 2009). HNT are suitable as catalytic supports (Machado, de Freitas Castro, Wypych, & Nakagaki, 2008;Massaro et al, 2014;, adsorbent nanomaterials for wastewater decontamination (Szczepanik & Słomkiewicz, 2016;Zhang et al, 2016), and nanofiller for sustainable packaging (Liu, Wu, Jiao, Xiong, & Zhou, 2013;Tescione, Buonocore, Stanzione, Oliviero, & Lavorgna, 2014).…”