Multibiomarker assessment of cerium dioxide nanoparticle (nCeO2) sublethal effects on two freshwater invertebrates, Dreissena polymorpha and Gammarus roeseli

Garaud, Maël; Trapp, Judith; Devin, Simon; Cossu-Leguille, Carole; Pain-Devin, Sandrine; Felten, Vincent; Giambérini, Laure

doi:10.1016/j.aquatox.2014.11.004

Cited by 43 publications

(29 citation statements)

References 61 publications

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“…However, due to a reduced dataset, this model has no predictive value, and should only be considered for its ability to synthetize and describe links between nanoparticle exposition and biomarker responses. Of the seven biomarkers identified, three were of particular significance (p<0.05; piGST mRNA levels, catalase activity, and hemocyte lysosomal system size) confirming our previous results (Garaud et al, 2015).…”

Section: Biological Effects Of Nceosupporting

confidence: 89%

“…We recently stressed the benefit of using integrated biomarker responses to study nCeO 2 effects and to identify biomarkers of interest (Garaud et al, 2015). In the present work, a very synthetic overview of the effects of nCeO 2 on mussel biology was provided using discriminant analysis and we were able i) to clearly differentiate control groups from nCeO 2exposed groups, ii) to show differential responses depending on nCeO 2 type and iii) to identify a reduced set of biomarkers sufficient to discriminate groups and their respective significance.…”

Section: Biological Effects Of Nceomentioning

confidence: 99%

“…Consequently, there is a need to better characterize the fate of nanoparticles in the exposure media and to relate that to their bioaccumulation, biotransformation and subsequent biological effects. To meet these goals, the French ANR MESONET program was launched with the aim of setting up adaptable freshwater mesocosms (Auffan et al, 2014b) in three French laboratories to study the fate and effects of manufactured nanoparticles in simplified ecosystems, using species belonging to different In the present study, biological effects were assessed using a multibiomarker approach, including responses related to various organism functions, and ranging from the molecular to the individual scale, allowing a better understanding of stressor mechanisms of action (Garaud et al, 2015). Such multibiomarker studies on the effects of nanoparticles on aquatic organisms are scarce, probably due to the difficulty to synthesize and analyze properly the complex data set (Guerlet et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

et al. 2016

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Exposures in realistic environmental conditions are essential to properly assess the effects of emerging pollutants on ecosystems. While ceria nanoparticles (nCeO2) production and use are expanding quickly, ecotoxicity studies remain very scarce. In this study, we set up experimental systems reproducing a simplified ecosystem to assess the effects of a chronic exposure to citrate-coated nCeO2 (ci-CeO2) and bare nCeO2 (ba-CeO2) on the freshwater mussel Dreissena polymorpha using an integrated multibiomarker approach. The fate of nanoparticles was tightly monitored to properly characterize the exposure. Organisms were exposed for 3 weeks and sampled weekly for biomarker analysis. Mussel filter-feeding activity resulted in significant removal of nCeO2 from the water column. At the same time, bioaccumulation was low, reaching its maximum in the first week. Mussels bioaccumulated ci-CeO2 three times more than ba-CeO2, probably due to coating-related differences in their behavior in the water column and in organisms. Meanwhile, biomarker results were integrated and synthesized using linear discriminant analysis, highlighting that pi-glutathione-S-transferase (piGST) mRNA, catalase (CAT) activity and lysosomal system were the most impacted of the seven biomarkers singled out by the discriminant analysis. These biomarker responses indicated that mussels exposed to both forms of nCeO2 were stressed and differentiate from the controls. Moreover, they responded differently to ba-CeO2 and ci-CeO2 exposure. However, biomarkers used in the experimental conditions of this study did not indicate severe nCeO2 toxicity on mussels, as cellular damage biomarkers and mussel filtering activity were left unimpaired. However, further studies are needed to investigate if the slight perturbations observed could lead to populational impacts in the long term.

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Section: Biological Effects Of Nceosupporting

confidence: 89%

Section: Biological Effects Of Nceomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

et al. 2016

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“…Acid phosphatase (ACP), lipid peroxidation (LOOH), lactate dehydrogenase (LDH), electron transport system (ETS) and energy reserves were measured on the S1 fractions, while total antioxidant capacity (TAC), catalase (CAT), glutathione peroxidase (GPxtot), and glutathione S-transferase (GST), caspase-3 (CASP-3) were measured on the cytosolic fraction (S2) by an automated colorimetric methods developed on the Konelab 20-XTi (Thermo Scientific) (Garaud et al, 2015(Garaud et al, , 2016. All technical procedures are described in detail in the Supplementary material.…”

Section: Energy Reserves Detoxification and Lipid Peroxidation (Lpo)mentioning

confidence: 99%

“…locomotion) responses, are appropriate tools to investigate sub-lethal effects and determine mechanisms of actions of AgNPs in aquatic invertebrates (Lagadic, 2002;Moore et al, 2004). These biomarkers are known to be early warning indicators used as a diagnostic or predictive tool for long-term effects that could occur at individual and/or population level (Garaud et al, 2015(Garaud et al, , 2016Jemec et al, 2008;Lagadic, 2002;Ulm et al, 2015;Vasseur and Leguille, 2004).…”

Section: Introductionmentioning

confidence: 98%

Gammarus fossarum (Crustacea, Amphipoda) as a model organism to study the effects of silver nanoparticles

Mehennaoui

Georgantzopoulou

Felten

et al. 2016

Science of The Total Environment

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Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated

Souza

Rocha

Franchi

2018

Advances in Experimental Medicine and Biology

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This chapter will present an original effort to summarize the relevant data about the cyto-genotoxicity induced by cerium dioxide nanoparticles (nanoceria) in physiologically (in vivo and in vitro) relevant models. In this way, this chapter should be extremely useful to everyone who wants to plan their research and publishing their results. Massive application of nanoceria at different fields is increasing year after year, and it is urgent to address and discuss their use and its safety-related issues. Specifically, the nanoceria are being designed for nanomedicine, cosmetics, polishing materials and additives for automotive fuels. Their unique properties include the ability to absorb UV radiation, antioxidant potential and the rapid exchange of valence between Ce and Ce ions associated to oxygen storage. In this chapter, the state of the art regarding the physicochemical properties of nanoceria, nanogenotoxicity detected by in vitro and in vivo systems and the general aspects in the cyto-genotoxic mechanism of nanoceria are summarized. The cyto-genotoxicity will be discussed in terms of evaluations by Comet assay, Micronucleus test, DNA damage response and oxidative stress detected in cell culture systems and in vivo test. We also described the dose dependent cyto-genotoxic effects of nanoceria based on their physical-chemical nature. Paradoxically, these particles have been characterized as either pro-oxidant or anti-oxidant in dependence of microenvironment and physiological conditions such as pH. Finally, this chapter will contribute to point out aspects of the development of new in vitro and in vivo methodologies to detect cyto-genotoxic effects of the nanoceria.

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Multibiomarker assessment of cerium dioxide nanoparticle (nCeO2) sublethal effects on two freshwater invertebrates, Dreissena polymorpha and Gammarus roeseli

Cited by 43 publications

References 61 publications

Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

Integrated assessment of ceria nanoparticle impacts on the freshwater bivalveDreissena polymorpha

Gammarus fossarum (Crustacea, Amphipoda) as a model organism to study the effects of silver nanoparticles

Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated

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