Gammarus fossarum (Crustacea, Amphipoda) as a model organism to study the effects of silver nanoparticles

Mehennaoui, Kahina; Georgantzopoulou, Anastasia; Felten, Vincent; Andreï, Jennifer; Garaud, Maël; Cambier, Sébastien; Serchi, Tommaso; Pain-Devin, Sandrine; Guérold, François; Audinot, Jean‐Nicolas; Giambérini, Laure; Gutleb, Arno C.

doi:10.1016/j.scitotenv.2016.06.068

Cited by 38 publications

(27 citation statements)

References 81 publications

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“…G. fossarum were collected at an unpolluted stream (49°48′24.9″ N and 06°04′53.2″ E, Schwaarzbaach, Colmar-berg, Luxembourg) 19 , 20 , 41 . Animals were collected using a hand net and were sorted in the field.…”

Section: Methodsmentioning

confidence: 99%

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Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda)

Mehennaoui¹,

Legay²,

Serchi³

et al. 2018

Sci Rep

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Gene expression profiling via RT-qPCR is a robust technique increasingly used in ecotoxicology. Determination and validation of optimal reference genes is a requirement for initiating RT-qPCR experiments. To our best knowledge, this study is the first attempt of identifying a set of reference genes for the freshwater crustacean Gammarus fossarum. Six candidate genes (Actin, TUB, UB, SDH, Clathrin and GAPDH) were tested in order to determine the most stable ones in different stress conditions and to increase the robustness of RT-qPCR data. SDH and Clathrin appeared as the most stable ones. A validation was performed using G. fossarum samples exposed for 15 days to AgNO3, silver nanoparticles (AgNPs) 40 nm and gold nanoparticles (AuNPs) 40 nm. Effects on HSP90 were evaluated and data normalized using Clathrin and SDH. A down-regulation of HSP90 was observed when G. fossarum were exposed to AuNPs 40 nm whereas no effects were observed when G. fossarum were exposed to AgNPs 40 nm. This study highlights the importance of the preliminary determination of suitable reference genes for RT-qPCR experiments. Additionally, this study allowed, for the first time, the determination of a set of valuable genes that can be used in other RT-qPCR studies using G. fossarum as model organism.

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Section: Methodsmentioning

confidence: 99%

“…In order to avoid influence of gender on the studied parameters, only adult males were kept for the experiment 17 , 42 . They were selected from precopula pairs or based on sexual dimorphism like gnatopode size 19 . Adult males were then acclimated to laboratory conditions 19 , 43 .…”

Section: Methodsmentioning

confidence: 99%

Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda)

Mehennaoui¹,

Legay²,

Serchi³

et al. 2018

Sci Rep

Self Cite

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“…Nanomaterials. Ma et al, (2016) A study selected G. fossarum to assess effects of well-characterized chemically synthesized silver nanoparticles (AgNPs) and "green" laboratory synthetized AgNPs by Mehennaoui et al, (2016…”

Section: Polychlorinated Biphenyls (Pcbsmentioning

confidence: 99%

Effects of Pollution on Freshwater Organisms

Choudri

Baawain²

2017

Water Environment Research

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This review covers research papers and scientific literature published in the year 2016 on the effects of anthropogenic pollutants on freshwater organisms. In the first, the review begins with two broad sections thate are mainly focused on research reviews and broad field studies and surveys. This is followed by reviews of research categorized in sections to reflect the pollutant class. These sections include wastewater, stormwater and non-point source pollution, nutrients, sediment cap materials and suspended clays, botanical extracts, surfactants, metals, persistent organic pollutants, pharmaceuticals, endocrine disruptors, pesticides, petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), ionic liquids, and nanomaterials. The final section of the review highlights the research published on describing innovations in the field of freshwater pollution research.

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“…It is a bottom-living, i.e., benthic, organism at the base of many temperate freshwater food webs [ 20 ]. Its functions for nutrient and organic matter cycling are well-studied and it is frequently used as a model organism for ecotoxicological studies [ 21 ]. We therefore assumed that Gammarus fossarum would be a relevant organism, whose feeding ecology and habitat would expose it to MPP entering the freshwater environment.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable and Petroleum-Based Microplastics Do Not Differ in Their Ingestion and Excretion but in Their Biological Effects in a Freshwater Invertebrate Gammarus fossarum

Straub

Hirsch

Burkhardt‐Holm

2017

IJERPH

156

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Research on the uptake and effects of bioplastics by aquatic organisms is still in its infancy. Here, we aim to advance the field by comparing uptake and effects of microplastic particles (MPP) of a biodegradable bioMPP (polyhydroxybutyrate (PHB)) and petroleum-based MPP (polymethylmethacrylate (PMMA)) in the freshwater amphipod Gammarus fossarum. Ingestion of both MPP in different particle sizes (32–250 µm) occurred after 24 h, with highest ingestion of particles in the range 32–63 µm and almost complete egestion after 64 h. A four-week effect-experiment showed a significant decrease of the assimilation efficiency in amphipods exposed to the petroleum-based MPP from week two onwards. The petroleum-based PMMA affected assimilation efficiency significantly in contrast to the biodegradable PHB, but overall differences in direct comparison of MPP types were small. Both MPP types led to a significantly lower wet weight gain relative to the control treatments. After four weeks, differences between both MPP types and silica, used as a natural particle control, were detected. In summary, these results suggest that both MPP types provoke digestive constraints on the amphipods, which go beyond those of natural non-palatable particles. This highlights the need for more detailed research comparing environmental effects of biodegradable and petroleum-based MPP and testing those against naturally occurring particle loads.

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Gammarus fossarum (Crustacea, Amphipoda) as a model organism to study the effects of silver nanoparticles

Cited by 38 publications

References 81 publications

Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda)

Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda)

Effects of Pollution on Freshwater Organisms

Biodegradable and Petroleum-Based Microplastics Do Not Differ in Their Ingestion and Excretion but in Their Biological Effects in a Freshwater Invertebrate Gammarus fossarum

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