Multibeam fluorescence diffuse optical tomography using upconverting nanoparticles

Liu, Haichun; Xu, Cuilian; Andersson‐Engels, Stefan

doi:10.1364/ol.35.000718

Cited by 20 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12 This upconversion (UC) ability provides advantages including autofluorescence rejection, 13 better light penetration and improved spatial image resolution. 7,14,15 So far, UCNPs have been successfully used in diverse biological applications such as photodynamic therapy (PDT), 8 microscopy, 9,10 bioanalytical assays, 16,17 diffuse optical imaging, 7,15,18,19 and multimodality imaging. 20 Although UCNPs have many beneficial properties for biological applications, a major challenge of their use is the power density dependent and relatively low QYs under the low excitation intensities required for these applications.…”

Section: Introductionmentioning

confidence: 99%

Balancing power density based quantum yield characterization of upconverting nanoparticles for arbitrary excitation intensities

et al. 2013

Self Cite

View full text Add to dashboard Cite

Upconverting nanoparticles (UCNPs) have recently shown great potential as contrast agents in biological applications. In developing different UCNPs, the characterization of their quantum yield (QY) is a crucial issue, as the typically drastic decrease in QY for low excitation power densities can either impose a severe limitation or provide an opportunity in many applications. The power density dependence of the QY is governed by the competition between the energy transfer upconversion (ETU) rate and the linear decay rate in the depopulation of the intermediate state of the involved activator in the upconversion process. Here we show that the QYs of Yb(3+) sensitized two-photon upconversion emissions can be well characterized by the balancing power density, at which the ETU rate and the linear decay rate have equal contributions, and its corresponding QY. The results in this paper provide a method to fully describe the QY of upconverting nanoparticles for arbitrary excitation power densities, and is a fast and simple approach for assessing the applicability of UCNPs from the perspective of energy conversion.

show abstract

Section: Introductionmentioning

confidence: 99%

Balancing power density based quantum yield characterization of upconverting nanoparticles for arbitrary excitation intensities

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Firstly, the QY is much higher for UCNPs at low photon density rates, which constitutes one main reason for the interest in them and removes the reliance of and restriction to focal volume excitation, thus broadening their field of application. 19,20 Secondly, the excitation for UC emission relies on intermediate energy levels, 21 complicating the process. This has led to some less successful attempts to utilize pulsed excitation for UCNPs in the past.…”

Section: Introductionmentioning

confidence: 99%

Deep tissue optical imaging of upconverting nanoparticles enabled by exploiting higher intrinsic quantum yield through use of millisecond single pulse excitation with high peak power

Liu

Dumlupinar

et al. 2013

Nanoscale

Self Cite

View full text Add to dashboard Cite

-Engels, S. (2013). Deep tissue optical imaging of upconverting nanoparticles enabled by exploiting higher intrinsic quantum yield through use of millisecond single pulse excitation with high peak power. Nanoscale, 5(20), 10034-10040. https://doi.org/10.1039/c3nr01917aRegistered Charity Number 207890 Accepted ManuscriptThis is an Accepted Manuscript, which has been through the RSC Publishing peer review process and has been accepted for publication.Accepted Manuscripts are published online shortly after acceptance, which is prior to technical editing, formatting and proof reading. This free service from RSC Publishing allows authors to make their results available to the community, in citable form, before publication of the edited article. This Accepted Manuscript will be replaced by the edited and formatted Advance Article as soon as this is available.To cite this manuscript please use its permanent Digital Object Identifier (DOI®), which is identical for all formats of publication.More information about Accepted Manuscripts can be found in the Information for Authors.Please note that technical editing may introduce minor changes to the text and/or graphics contained in the manuscript submitted by the author(s) which may alter content, and that the standard Terms & Conditions and the ethical guidelines that apply to the journal are still applicable. In no event shall the RSC be held responsible for any errors or omissions in these Accepted Manuscript manuscripts or any consequences arising from the use of any information contained in them. We have accomplished deep tissue optical imaging of upconverting nanoparticles at 800 nm, using millisecond single pulse excitation with high peak power. This is achieved by carefully choosing the pulse parameters, derived from time-resolved rateequation analysis, which result in higher intrinsic quantum yield that is utilized by upconverting nanoparticles for generating this near infrared upconversion emission. The pulsed excitation approach thus promises previously unreachable imaging depths and shorter data acquisition times compared with continuous wave excitation, while simultaneously keeping the possible thermal side-effects of the excitation light moderate. These key results facilitate means to break through the general shallow depth limit of upconverting-nanoparticle-based fluorescence techniques, necessary for a range of biomedical applications, including diffuse optical imaging, photodynamic therapy and remote activation of biomolecules in deep tissues. www.rsc.org/nanoscale

show abstract

“…Interestingly, the nonlinear power dependence of the UCNPs can be exploited to increase the information density for luminescence diffuse optical tomography from a given volume by carefully designing the excitation scheme to include multi‐beam excitation. This was demonstrated by us by implementing dual‐beam luminescence diffuse optical tomography with UCNPs . It was shown that the use of two excitation beams for a group of quadratic UCNPs resulted in orthogonal data due to the “interference ” of the two individual excitation profiles in the UCNPs.…”

Section: Excitation Manipulation Of Upconversion Nanoparticlesmentioning

confidence: 99%

“…It was shown that the use of two excitation beams for a group of quadratic UCNPs resulted in orthogonal data due to the “interference ” of the two individual excitation profiles in the UCNPs. The additional information obtained led to a more correct representation of the true UCNP distribution . Such benefit cannot be found when using linear fluorophores.…”

Section: Excitation Manipulation Of Upconversion Nanoparticlesmentioning

confidence: 99%

Photon Upconversion Kinetic Nanosystems and Their Optical Response

Liu

Huang

Valiev

et al. 2017

Laser & Photonics Reviews

Self Cite

View full text Add to dashboard Cite

Lanthanide-doped photon upconversion nanoparticles (UCNPs) are capable of converting low-intensity near-infrared light to UV and visible emission through the synergistic effects of light excitation and mutual interactions between doped ions. UCNPs have attracted strong interest as unique spectrum converters and found a multitude of applications in areas like biomedical imaging, energy harvesting and information technology. UCNPs are distinct from many other types of luminescent materials in terms of the involvement of a host lattice and multiple optical centers, i.e., trivalent lanthanide ions with manyfolds of accessible long-lived energy states, in individual nanoparticles. The mutual interactions between these optical centers, i.e., sequential energy transfers, make them operate as an integrated unit and co-determine the luminescence kinetics and other optical properties of the individual nanoparticle. Thus, each nanoparticle consititutes a kinetic optical system. In this work, we explore UCNPs from the outset of being such kinetic optical systems and review their physical formation, the underlying photophysics, macroscopic statistical description, and their response to various optical stimuli in the spectral, polarization, intensity, temporal and frequency domains, and demonstrate ways that their optical output can be optimized by manipulating the excitation schemes. Our review highlights upconversion nanotechnology as an interdisciplinary field across chemistry, physics and biomedical engineering, with great future possibilities, flexibility and ramifications. We outline some of the potential directions of upconversion nanoparticle research.

show abstract

Multibeam fluorescence diffuse optical tomography using upconverting nanoparticles

Cited by 20 publications

References 18 publications

Balancing power density based quantum yield characterization of upconverting nanoparticles for arbitrary excitation intensities

Balancing power density based quantum yield characterization of upconverting nanoparticles for arbitrary excitation intensities

Deep tissue optical imaging of upconverting nanoparticles enabled by exploiting higher intrinsic quantum yield through use of millisecond single pulse excitation with high peak power

Photon Upconversion Kinetic Nanosystems and Their Optical Response

Contact Info

Product

Resources

About