Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum

Characterization of a Lactobacillus gasseri JCM 1131 T Lipoteichoic Acid with a Novel Glycolipid Anchor Structure

“…The peaks detected by 1 H NMR and 13 C distortionless enhancement by polarization transfer with an angle parameter of 135°(DEPT-135) NMR (see Fig. S1A, B, and C in the supplemental material) were attributable to the GroP residue and the 2-alanyl-GroP (2-AlaGroP) residue with a substitution at the C-2 hydroxyl group of glycerol to D-alanine (Table 1), as deduced from previous reports (9,10,12). No hexoses were detected as substituents.…”

“…T LTA were supported by a difference of 162 in molecular mass, corresponding to one hexose residue, compared to the mass spectrum of L. plantarum LTA (9). No equivalent peaks corresponding to Hex 3 structures were observed in L. gasseri JCM 1131 T (Fig.…”

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

“…UT indicates untreated cells. that the two LTAs have different glycolipid anchors (Jang et al, 2011). The biological roles of LTA glycolipid anchors have drawn great attention, since they are known to be key molecules that trigger innate immunity in hosts.…”

Anti-inflammatory potential of probiotics: lipoteichoic acid makes a difference

“…LTA from L. plantarum KCTC10887BP even acted as an antagonist of S. aureus LTA-induced TNF production in monocytic cells in a TLR2/ CD14-dependent manner [28], of LPS-induced TNF production in monocytic cells and endotoxin shock in mice [29] and of Shigella flexneri peptidoglycan-induced inflammation in monocytic cells [30]. Recently, multi-spectrometric analyses of this L. plantarum LTA were compared with those of S. aureus LTA [31]. These analyses revealed that the major differences lie in the numbers (third acyl chain in L. plantarum LTA) and degree of saturation of the acyl chain in the glycolipid moiety (unsaturated fatty acids in L. plantarum), and the sugar substituents on the Gro-P chains (glucose and galactose sugars in L. plantarum versus GlcNAc in S. aureus), whereas they showed similar levels of D-ala.…”