Multi-sheet excitation and imaging of flow driven samples in an LSFM with a modified multi-focal diffractive lens

Hofmann, Martin; Ghebjagh, Shima Gharbi; Lemke, Karen; Sinzinger, Stefan

doi:10.1364/cosi.2021.cm1a.7

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…Uniform laser-beam irradiation is needed e.g. for laser microfabrication, laser welding and multi-location, parallel and simultaneous processing but is increasingly also interesting in imaging applications such as light sheet microscopy [2] . An efficient method for designing the phase * e-mail: shima.gharbi-gebjagh@tu-ilmenau.de distribution for beam shaping applications is based on the iterative Fourier transform algorithms (IFTA), suggested by Gerchberg and Saxton.…”

Section: Design Of Array Illuminatorsmentioning

confidence: 99%

Micro-diffractive optical element arrays for beam shaping

2022

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We describe the design of Fourier type array generators and beam shapers as periodic configurations of refractive-diffractive optical elements in microscale to provide specific beam shaping and imaging functionaliies. We investigate how the addition of micro-nanostructures to regular microstructure arrays enables new degrees of freedom for the design of micro-optical systems, in combination with adapted fabrication techniques yields a better optical performance and leads to enhancement of the array concept, uniformity and efficiency.

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Section: Design Of Array Illuminatorsmentioning

confidence: 99%

Micro-diffractive optical element arrays for beam shaping

2022

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Introduction to Fluorescence Microscopy

Macháň

2022

Springer Series on Fluorescence

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Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope

Hofmann

Ghebjagh²,

Chen³

et al. 2022

EPJ Web Conf.

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Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along an inclined tube for applications in flow-driven imaging. The element, which is based on a multi-focal Fresnel zone plate and a linear grating, generates in combination with a refractive cylindrical lens five thin light sheets of equal intensity.

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Multi-sheet excitation and imaging of flow driven samples in an LSFM with a modified multi-focal diffractive lens

Abstract: Five light sheets of equal intensity are generated within the tube of a flow driven Light Sheet Fluorescence Microscope (LSFM). In combination with a second multi-focal lens multiplane imaging on a single chip is provided.

Cited by 4 publications

References 7 publications

Micro-diffractive optical element arrays for beam shaping

Micro-diffractive optical element arrays for beam shaping

Introduction to Fluorescence Microscopy

Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope

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