Multi residue screening of intact testosterone esters and boldenone undecylenate in bovine hair using liquid chromatography electrospray tandem mass spectrometry

Determination of hormonal growth promoters in bovine hair: Comparison of liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry methods for estradiol benzoate and nortestosterone decanoate

“…Similarly to the approach taken by Nielen et al [15] for testosterone and boldenone esters, the method was expanded to include medroxyprogesterone acetate, testosterone propionate, testosterone decanoate, nortestosterone decanoate and boldenone undecylenate. The method was developed and optimised to give maximum recovery and minimum ion suppression for each of the analytes.…”

“…Recent advances in liquid chromatography-mass spectrometry (LC-MS/MS) technology have improved the robustness and potential sensitivity of this method and methods for detecting steroid esters in bovine hair using LC-MS/MS have been published [14,15]. However, ion suppression [16] may be a problem for LC-MS/MS analysis, in contrast to GC-MS/MS, and lead to various consequences, all affecting to a high extent the different aspects of the expected analytical result (e.g.…”

Detection of testosterone propionate administration in horse hair samples

“…The detection limit for testosterone propionate achieved on the two TQ mass spectrometers defined as 3 S/N was about 0.5 pg mg −1 , which is appropriate [5,14,16] for the analysis of anabolic esters in hair.…”

“…More recently, Gratacos-Cubarsi et al [5] Nielen et al [14], have shown the great possibilities of bovine hair analysis in detecting intact testosterone esters and boldenone undecylenate by GC/MS or LC/MS. Regarding steroid analysis in horse hair samples, boldenone was detected in the mane and tail retrospectively for up to 12 months after boldenone undecylenate administration to two different horses [15].…”

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

“…The gradient was modified to: 0 min 0% B, 0.50 min 0% B, 6 min 100% B, 7.5 min 100% B, 7.6 min 0% B, 10 min 0% B, at a flow rate of 0.4 mL/min. The injection volume was 40 L. The mass spectrometer was operated by using the ion source and acquisition parameters as described in reference [18], with the following modifications: instead of two, three MRM transitions were acquired for each analyte. Furthermore, different colli-sion energies, ranging from 5 to 45 eV, were used for each MRM transition.…”