Multi-residue detection of pesticides using a sensitive immunochip assay based on nanogold enhancement

Lan, Meijing; Guo, Yirong; Zhao, Ying; Liu, Yihua; Gui, Wenjun; Zhu, Guonian

doi:10.1016/j.aca.2016.07.044

Cited by 47 publications

(17 citation statements)

References 36 publications

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“…Four mycotoxin antibodies were characterized and screened using competitive indirect enzyme-linked immunosorbent assay (iELISA) [ 19 ]. Monoclonal antibodies against OTA and ZEN had the same features as those characterized previously [ 22 , 23 ].…”

Section: Resultsmentioning

confidence: 99%

“…In this system, signal responses produced by antigen (Ag)-antibody (Ab) interaction is observed by a confocal laser scanner and image extraction software. This high-throughput detection platform has the advantages of sensitivity, rapidity, and accuracy, and has great potential for applications in agriculture and food chemicals, such as pesticides, pathogens, antibiotics, and mycotoxins [ 18 , 19 , 20 , 21 ]. However, an antibody microarray immunoassay based on the direct competition strategy and the biotin-streptavidin signal amplification system for multiple mycotoxins detection has not yet been reported in the available literature.…”

Section: Introductionmentioning

confidence: 99%

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Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Zhang

Wang

Fang

et al. 2018

Toxins

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We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Zhang

Wang

Fang

et al. 2018

Toxins

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“…To further increase the number of target analytes, microarray biochips have become a promising tool for monitoring pesticide and veterinary drug residues. A microarray chip has been developed for the simultaneous detection of seven pesticides (triazophos, methyl-parathion, fenpropathrin, carbofuran, thiacloprid, chlorothalonil, and carbendazim) [57]. Seven antigens were dispensed onto separate test zones of a nitrocellulose membrane.…”

Section: Preparation and Application Of Multi-antibodiesmentioning

confidence: 99%

“…Due to the summation effect in formation of a two-monoclonal antibodies sandwich complex, simultaneous determination of 14 SAs was achieved in turkey muscle and milk samples [58]. methyl-parathion, fenpropathrin, carbofuran, thiacloprid, chlorothalonil, and carbendazim) [57]. Seven antigens were dispensed onto separate test zones of a nitrocellulose membrane.…”

Section: Preparation and Application Of Multi-antibodiesmentioning

confidence: 99%

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

et al. 2020

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The excessive use or abuse of pesticides and veterinary drugs leads to residues in food, which can threaten human health. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of pesticide and veterinary drug residues, which can be applied as screening techniques for food safety monitoring and detection. Recent developments related to rapid multi-residue detection methods for pesticide and veterinary drug residues are reviewed herein. Methods based on different recognition elements or the inherent characteristics of pesticides and veterinary drugs are described in detail. The preparation and application of three broadly specific recognition elements—antibodies, aptamers, and molecular imprinted polymers—are summarized. Furthermore, enzymatic inhibition-based sensors, near-infrared spectroscopy, and SERS spectroscopy based on the inherent characteristics are also discussed. The aim of this review is to provide a useful reference for the further development of rapid multi-analyte analysis of pesticide and veterinary drug residues.

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“…Visual assessment or a simple portable spectrometer make possible a potential on-site simple detection. A flexible, simple and cost-effective nitrocellulose membrane-based colorimetric immunochip assay for the simultaneous detection of seven pesticides from six different chemical groups (triazophos, methyl-parathion, fenpropathrin, carbofuran, thiacloprid, chlorothalonil, and carbendazim) was developed by Lan et al [ 79 ]. The method is based on a secondary Ab-gold conjugate as universal reporter and AuNPs deposition for signal enhancement.…”

Section: Immunosensorsmentioning

confidence: 99%

Affinity Sensing Strategies for the Detection of Pesticides in Food

Capoferri

Pelle

Carlo

et al. 2018

Foods

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This is a review of recent affinity-based approaches that detect pesticides in food. The importance of the quantification and monitoring of pesticides is firstly discussed, followed by a description of the different approaches reported in the literature. The different sensing approaches are reported according to the different recognition element used: antibodies, aptamers, or molecularly imprinted polymers. Schemes of detection and the main features of the assays are reported and commented upon. The large number of affinity sensors recently developed and tested on real samples demonstrate that this approach is ready to be validated to monitor the amount of pesticides used in food commodities.

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Multi-residue detection of pesticides using a sensitive immunochip assay based on nanogold enhancement

Cited by 47 publications

References 36 publications

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

Affinity Sensing Strategies for the Detection of Pesticides in Food

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