Multi-Oscillatory Control of Eclosion and Oviposition Rhythms in<i>Drosophila melanogaster</i>: Evidence from Limits of Entrainment Studies

Paranjpe, Dhanashree; Anitha, D.; Joshi, Amitabh; Sharma, Vijay Kumar

doi:10.1081/cbi-200026463

Cited by 11 publications

(4 citation statements)

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“…Another striking difference between the three rhythms is that egg laying continued to be rhythmic under LL (Sheeba et al, 2001a), while locomotor activity and eclosion became arrhythmic (Saunders, 1982). Even the limits of entrainment of the egg-laying rhythm were quite different from those of locomotor activity and eclosion rhythms (Paranjpe et al, 2004). These studies thus suggest that separate timing systems regulate locomotor activity, eclosion, and egglaying rhythms in D. melanogaster.…”

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confidence: 74%

Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm inDrosophila melanogaster

2006

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The authors report the results of their study aimed at investigating the consequence of targeted ablation of ventral lateral neurons (LN(v)s--neurons regulating eclosion and locomotor activity rhythms) and genetic disruption of pigment-dispersing factor (PDF--an important output of circadian clocks) on the egg-laying rhythm of Drosophila melanogaster. The results clearly suggest that genetic ablation of LN(v)s and loss of function mutation of PDF abolish eclosion and locomotor activity rhythms, whereas the egg-laying rhythm continues unabated. Furthermore, the results also demonstrate that the period of egg-laying rhythm remains unchanged under different ambient temperatures and nutrition levels, suggesting that the egg-laying rhythm of D. melanogaster is temperature and nutrition compensated. Based on these results, the authors conclude that the egg-laying rhythm in D. melanogaster is regulated by non-LN(v)-based, non-PDF-mediated circadian clocks.

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confidence: 74%

Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm inDrosophila melanogaster

2006

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“…Photoperiod along with temperature has been shown to play a role in the regulation of diapause in clock mutant females of D. triauraria (Yamada & Yamamoto, 2011), while egg-laying rhythm under shorter LD cycles has been reported as showing more disrupted activity (Gruwez et al, 1971). Previous studies where egg-laying rhythm in Drosophila females was assayed under LD cycles of different period lengths have reported consolidation of egg-laying towards the night and a tendency to entrain better to long period LD cycles (Paranjpe et al, 2004). These studies seem to suggest that mating, clock genes, temperature and light, can all modulate the egglaying rhythm under periodic environments, though their combined effects may lead to a more irregular eggoutput, as is the case of group-3 females under LD cycles.…”

Section: Discussionmentioning

confidence: 96%

“…Among the circadian rhythms in D. melanogaster studied thus far, egg-laying rhythm is found to be unique in several ways: (i) the rhythm persists under constant light (LL) conditions in the laboratory in 50-90% females (Kannan et al, 2012;McCabe & Birley, 1998;Sheeba et al, 2001), wherein most other rhythms such as adult emergence and activity/rest are abolished (Chandrashekaran & Loher, 1969;Konopka & Pittendrigh, 1989), (ii) the of the rhythm is considerably longer and more variable than those of adult emergence and activity/rest rhythms (Sheeba et al, 2001) and (iii) this rhythm differs from the other two rhythms in terms of the limits of entrainment to LD cycles (Paranjpe et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Rhythmic egg-laying behaviour in virgin females of fruit fliesDrosophila melanogaster

Menon

Varma

Sharma

2013

Chronobiology International

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Fruit fly Drosophila melanogaster females display rhythmic egg-laying under 12:12 h light/dark (LD) cycles which persists with near 24 h periodicity under constant darkness (DD). We have shown previously that persistence of this rhythm does not require the neurons expressing pigment dispersing factor (PDF), thought to be the canonical circadian pacemakers, and proposed that it could be controlled by peripheral clocks or regulated/triggered by the act of mating. We assayed egg-laying behaviour of wild-type Canton S (CS) females under LD, DD and constant light (LL) conditions in three different physiological states; as virgins, as females allowed to mate with males for 1 day and as females allowed to mate for the entire duration of the assay. Here, we report the presence of a circadian rhythm in egg-laying in virgin D. melanogaster females. We also found that egg-laying behaviour of 70 and 90% females from all the three male presence/absence protocols follows circadian rhythmicity under DD and LL, with periods ranging between 18 and 30 h. The egg-laying rhythm of all virgin females synchronized to LD cycles with a peak occurring soon after lights-off. The rhythm in virgins was remarkably robust with maximum number of eggs deposited immediately after lights-off in contrast to mated females which show higher egg-laying during the day. These results suggest that the egg-laying rhythm of D. melanogaster is endogenously driven and is neither regulated nor triggered by the act of mating; instead, the presence of males results in reduction in entrainment to LD cycles.

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“…The results of these experiments are expected to provide an estimate of the intrinsic rate of pre-adult development in the presence and absence of circadian gating. For example, in DD, the pre-adult development time is known to be governed by circadian gate (associated with clocks) [21], while in LL condition circadian gating is completely abolished, and therefore time taken by the flies to complete pre-adult development in LL would solely depend upon the intrinsic rate of development. The results provide interesting insights into the link between circadian rhythms and pre-adult developmental time in fruit flies D. melanogaster .…”

Section: Introductionmentioning

confidence: 99%

Selection for early and late adult emergence alters the rate of pre-adult development in Drosophila melanogaster

et al. 2006

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BackgroundCircadian clocks have been implicated in the regulation of pre-adult development of fruit flies Drosophila melanogaster. It is believed that faster clocks speed up development and slower clocks slow it down. We established three sets of D. melanogaster populations (early, control and late). The early and late populations were raised by selecting for flies that emerged either in the morning or in the evening under 12:12 hr light/dark (LD) cycles. After 75 generations of selection, the time course and waveform of the adult emergence and activity rhythms of the early and the late populations diverged from each other as well as from the controls. In this paper, we report the consequence of this selection on the rate of pre-adult development.ResultsWe assayed the pre-adult development time of the selected and control populations under 12:12 hr LD cycles and constant darkness (DD). Under LD cycles, the early populations develop faster than the controls, while the late populations develop slower than the controls. Although flies take longer to develop under DD than in LD, the relative differences between the mean development times of the selected and control populations remain unaltered in DD. In a separate experiment designed to investigate the effect of time of egg collection and experimental conditions on the duration of pre-adult stage, we assayed the development time of the selected and control populations by collecting eggs at different times of the day (morning and evening) and by assaying their pre-adult development time under constant light (LL), LD, and DD conditions. Irrespective of the time of egg collection and assay light regime, the late flies continue to develop slower than the early flies.ConclusionThe results of our study clearly indicate that selection on the timing of adult emergence alters the rate of pre-adult development in D. melanogaster. The timing of egg collection as well as assay light regime does not have any measurable effect on the relative differences between the developmental rates of the early and the late flies. Taken together these results appear to suggest that pleiotropic effects of clock genes mediate correlated changes in the timing of adult emergence and the rate of pre-adult development in D. melanogaster.

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Multi-Oscillatory Control of Eclosion and Oviposition Rhythms inDrosophila melanogaster: Evidence from Limits of Entrainment Studies

Cited by 11 publications

References 34 publications

Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm inDrosophila melanogaster

Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm inDrosophila melanogaster

Rhythmic egg-laying behaviour in virgin females of fruit fliesDrosophila melanogaster

Selection for early and late adult emergence alters the rate of pre-adult development in Drosophila melanogaster

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