Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm in<i>Drosophila melanogaster</i>

Howlader, Gitanjali; Paranjpe, Dhanashree; Sharma, Vijay Kumar

doi:10.1177/0748730405282882

Cited by 27 publications

(23 citation statements)

References 22 publications

(32 reference statements)

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“…The GAL80 ts temperature-sensitive mutant protein that blocks GAL4 activity at 18°C, the permissive temperature, while at 30°C, the restrictive temperature, GAL4 is not repressed since the GAL80 protein is inactive. However, temperature changes can result in unintended physiological effects including alterations in aging or lifespan [5], [6], [7], [8], [9], circadian rhythm [10], [11], [12], [13], [14], sleep [15], reproduction [16], [17], development [18], [19], [20], [21], learning and memory [19], [22], olfactory perception [23], and can induce mutations [24]. Furthermore, our own study found that some UAS lines have “leaky” gene expression at higher temperatures resulting in confounding GAL4 independent phenotypes.…”

Section: Introductionmentioning

confidence: 99%

A Hormone Receptor-Based Transactivator Bridges Different Binary Systems to Precisely Control Spatial-Temporal Gene Expression in Drosophila

Kuo

Hsu

et al. 2012

PLoS ONE

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The GAL4/UAS gene expression system is a precise means of targeted gene expression employed to study biological phenomena in Drosophila. A modified GAL4/UAS system can be conditionally regulated using a temporal and regional gene expression targeting (TARGET) system that responds to heat shock induction. However heat shock-related temperature shifts sometimes cause unexpected physiological responses that confound behavioral analyses. We describe here the construction of a drug-inducible version of this system that takes advantage of tissue-specific GAL4 driver lines to yield either RU486-activated LexA-progesterone receptor chimeras (LexPR) or β-estradiol-activated LexA-estrogen receptor chimeras (XVE). Upon induction, these chimeras bind to a LexA operator (LexAop) and activate transgene expression. Using GFP expression as a marker for induction in fly brain cells, both approaches are capable of tightly and precisely modulating transgene expression in a temporal and dosage-dependent manner. Additionally, tissue-specific GAL4 drivers resulted in target gene expression that was restricted to those specific tissues. Constitutive expression of the active PKA catalytic subunit using these systems altered the sleep pattern of flies, demonstrating that both systems can regulate transgene expression that precisely mimics regulation that was previously engineered using the GeneSwitch/UAS system. Unlike the limited number of GeneSwitch drivers, this approach allows for the usage of the multitudinous, tissue-specific GAL4 lines for studying temporal gene regulation and tissue-specific gene expression. Together, these new inducible systems provide additional, highly valuable tools available to study gene function in Drosophila.

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Section: Introductionmentioning

confidence: 99%

A Hormone Receptor-Based Transactivator Bridges Different Binary Systems to Precisely Control Spatial-Temporal Gene Expression in Drosophila

Kuo

Hsu

et al. 2012

PLoS ONE

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“…Cyclic olfactory responses do not depend on the LNs or Pigment Dispersing Factor, but instead depend on the antennal neurons [45,46]. Egg-laying rhythms also appear to be regulated independently of the LN v s and Pigment Dispersing Factor [47]. Thus, the image of the circadian clock as a single entity is transforming into a more complex model.…”

Section: Discussionmentioning

confidence: 99%

Integration of Light and Temperature in the Regulation of Circadian Gene Expression in Drosophila

et al. 2007

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Circadian clocks are aligned to the environment via synchronizing signals, or Zeitgebers, such as daily light and temperature cycles, food availability, and social behavior. In this study, we found that genome-wide expression profiles from temperature-entrained flies show a dramatic difference in the presence or absence of a thermocycle. Whereas transcript levels appear to be modified broadly by changes in temperature, there is a specific set of temperature-entrained circadian mRNA profiles that continue to oscillate in constant conditions. There are marked differences in the biological functions represented by temperature-driven or circadian regulation. The set of temperature-entrained circadian transcripts overlaps significantly with a previously defined set of transcripts oscillating in response to a photocycle. In follow-up studies, all thermocycle-entrained circadian transcript rhythms also responded to light/dark entrainment, whereas some photocycle-entrained rhythms did not respond to temperature entrainment. Transcripts encoding the clock components Period, Timeless, Clock, Vrille, PAR-domain protein 1, and Cryptochrome were all confirmed to be rhythmic after entrainment to a daily thermocycle, although the presence of a thermocycle resulted in an unexpected phase difference between period and timeless expression rhythms at the transcript but not the protein level. Generally, transcripts that exhibit circadian rhythms both in response to thermocycles and photocycles maintained the same mutual phase relationships after entrainment by temperature or light. Comparison of the collective temperature- and light-entrained circadian phases of these transcripts indicates that natural environmental light and temperature cycles cooperatively entrain the circadian clock. This interpretation is further supported by comparative analysis of the circadian phases observed for temperature-entrained and light-entrained circadian locomotor behavior. Taken together, these findings suggest that information from both light and temperature is integrated by the transcriptional clock mechanism in the adult fly head.

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“…Females were allowed to lay eggs on yeasted agar plates for 6 hours at 25°C or for 4 hours at 18/29°C. Only eggs laid during the afternoon were scored in order to reduce variability due to rhythmic behavior [19]. The number of eggs laid in each plate was normalized by the number of tested females.…”

Section: Methodsmentioning

confidence: 99%

M6 Membrane Protein Plays an Essential Role in Drosophila Oogenesis

et al. 2011

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We had previously shown that the transmembrane glycoprotein M6a, a member of the proteolipid protein (PLP) family, regulates neurite/filopodium outgrowth, hence, M6a might be involved in neuronal remodeling and differentiation. In this work we focused on M6, the only PLP family member present in Drosophila, and ortholog to M6a. Unexpectedly, we found that decreased expression of M6 leads to female sterility. M6 is expressed in the membrane of the follicular epithelium in ovarioles throughout oogenesis. Phenotypes triggered by M6 downregulation in hypomorphic mutants included egg collapse and egg permeability, thus suggesting M6 involvement in eggshell biosynthesis. In addition, RNAi-mediated M6 knockdown targeted specifically to follicle cells induced an arrest of egg chamber development, revealing that M6 is essential in oogenesis. Interestingly, M6-associated phenotypes evidenced abnormal changes of the follicle cell shape and disrupted follicular epithelium in mid- and late-stage egg chambers. Therefore, we propose that M6 plays a role in follicular epithelium maintenance involving membrane cell remodeling during oogenesis in Drosophila.

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Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm inDrosophila melanogaster

Cited by 27 publications

References 22 publications

A Hormone Receptor-Based Transactivator Bridges Different Binary Systems to Precisely Control Spatial-Temporal Gene Expression in Drosophila

A Hormone Receptor-Based Transactivator Bridges Different Binary Systems to Precisely Control Spatial-Temporal Gene Expression in Drosophila

Integration of Light and Temperature in the Regulation of Circadian Gene Expression in Drosophila

M6 Membrane Protein Plays an Essential Role in Drosophila Oogenesis

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