Multi-omic approach to decipher the impact of skincare products with pre/postbiotics on skin microbiome and metabolome

Abstract

IntroductionAlthough pre/pro/postbiotics have become more prevalent in dermatologic and cosmetic fields, the mode of action when topically applied is largely unknown. A multi-omic approach was applied to decipher the impact of the skincare products with pre/postbiotics on skin microbiome and metabolome.MethodsSubjects with dry skin applied a body wash and body lotion with or without pre/postbiotics for 6 weeks. Skin hydration was measured at baseline, 3 and 6 weeks. Skin swabs were collected for 16S rRNA gene sequencing, metagenomics and metabolomics analysis.ResultsSkin hydration significantly increased in both groups. The prebiotic group significantly reduced opportunistic pathogens, e.g., Pseudomonas stutzeri and Sphingomonas anadarae, and increased the commensals, e.g., Staphylococcus equorum, Streptococcus mitis, Halomonas desiderata. Bacterial sugar degradation pathways were enriched in the prebiotic group, while fatty acid biosynthesis pathways were reduced in control. The changes on skin metabolome profiles by the products were more prominent. The prebiotic group performed greater modulation on many clinically-relevant metabolites compared to control. Correlation analysis showed H. desiderata and S. mitis positively correlated with skin hydration, P. stutzeri and S. anadarae negatively correlated with the metabolites that are positively associated with skin hydration improvement.ConclusionThis holistic study supported a hypothesis that the pre/postbiotics increased skin hydration through the modulation of skin microbiome, metabolic pathways and metabolome.

Microbiome and lipidomic analysis reveal the interplay between skin bacteria and lipids in a cohort study

“…16S rRNA gene sequencing V1-3 hypervariable region of 16S rRNA gene sequencing was conducted by RTL Genomics (Lubbock, Texas) as previously described (Li et al, 2023). DNA was extracted via KingFisher FLEX instrument (ThermoFisher Scientific, Inc., Waltham, Massachusetts) and using Zymo ZR-96 magbead kit (Zymo Research, Irvine, California) following manufacturer's instructions.…”

Past, present, and future of microbiome-based therapies

“…Most of the currently existing postbiotics are derived from bacteria of the Lactobacillus or Bifidobacterium bacterial genera, but also from yeasts such as Saccharomyces cerevisiae, mainly from the fermentation of these bacteria carried out in industrial processes [100][101][102][103][104][105] . Postbiotics are commonly metabolites or polysaccharides, substances with antioxidants, anti-inflammatory or even immunomodulatory properties [15,95,100] .…”