Co-occurrence network analysis reveals the alterations of the skin microbiome and metabolome in adults with mild to moderate atopic dermatitis

P. Gomes, Paulo Wender; Mannochio-Russo, Helena; Mao, Junhong; Zhao, Haoqi Nina; Ancira, Jacob; Tipton, Craig D.; Dorrestein, Pieter C.; Li, Min

doi:10.1128/msystems.01119-23

Cited by 3 publications

(1 citation statement)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 57 healthy participants from Piscataway, New Jersey, USA were recruited. The study design was illustrated in Figure 1 ( Gomes et al, 2023 ). The informed consent was signed by each participant.…”

Section: Methodsmentioning

confidence: 99%

Microbiome and lipidomic analysis reveal the interplay between skin bacteria and lipids in a cohort study

Li,

Kopylova,

Mao

et al. 2024

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Human skin acts as a protective barrier between the body and the external environment. Skin microbiome and intercellular lipids in the stratum corneum (SC) are essential for maintaining skin barrier function. However, the interplay between skin bacteria and the lipids is not fully understood. In this study, we characterized the skin microbiome and SC lipid profiles from the forearm and face in a cohort of 57 healthy participants. 16S rRNA gene sequencing showed the skin microbial composition is significantly different between body locations and genders. Female forearm samples have the highest microbial diversity. The relative abundance of Staphylococcus hominis, Micrococcus luteus, Corynebacterium tuberculostearicum, Finegoldia magna, and Moraxellaceae sp. are significantly higher in the forearm than the face. The predictive functional analysis of 16S rRNA gene sequencing by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) and ANCOM-BC showed different bacterial metabolic pathway profiles between body locations or genders, and identified 271 differential pathways, including arginine and polyamine biosynthesis, chorismate biosynthesis pathways, which are more abundant in the female forearm, and sulfur oxidation pathway, which is more abundant in the male face. The SC lipid profiles differ between the body locations as well. Total free fatty acids (FFA), cholesterol sulfate and sphingosine are more abundant in the face. Dihydro-/6-hydroxy/phyto-ceramides are more abundant in the forearm. The correlation analysis of 16S rRNA gene sequencing and lipids revealed novel interplay between the bacteria and skin lipids. Shannon entropy and S. hominis negatively correlated with FFA, cholesterol sulfate and sphingosine; while positively correlated with dihydro-/6-hydroxy/phyto-ceramides. The correlation of predictive pathway profiles and lipids identified pathways involved in amino acids metabolism, carbohydrates degradation, aromatic compounds metabolism and fatty acid degradation metabolism are positively correlated with dihydro-/6-hydroxy/phyto-ceramides and negatively correlated with FFA, cholesterol sulfate and sphingosine. This study provides insights on the potential correlation between skin microbiome and lipids.

show abstract

Section: Methodsmentioning

confidence: 99%

Microbiome and lipidomic analysis reveal the interplay between skin bacteria and lipids in a cohort study

Li,

Kopylova,

Mao

et al. 2024

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

Staphylococcus aureus colonizing the skin microbiota of adults with severe atopic dermatitis exhibits genomic diversity and convergence in biofilm traits

Sivori,

Cavallo,

Truglio

et al. 2024

Biofilm

View full text Add to dashboard Cite

Environmental and structural factors associated with bacterial diversity in household dust across the Arizona-Sonora border

Benton,

Lopez-Galvez,

Herman

et al. 2024

Sci Rep

View full text Add to dashboard Cite

We previously reported that asthma prevalence was higher in the United States (US) compared to Mexico (MX) (25.8% vs. 8.4%). This investigation assessed differences in microbial dust composition in relation to demographic and housing characteristics on both sides of the US–MX Border. Forty homes were recruited in the US and MX. Home visits collected floor dust and documented occupants’ demographics, asthma prevalence, housing structure, and use characteristics. US households were more likely to have inhabitants who reported asthma when compared with MX households (30% vs. 5%) and had significantly different flooring types. The percentage of households on paved roads, with flushing toilets, with piped water and with air conditioning was higher in the US, while dust load was higher in MX. Significant differences exist between countries in the microbial composition of the floor dust. Dust from Mexican homes was enriched with Alishewanella, Paracoccus, Rheinheimera genera and Intrasporangiaceae family. A predictive metagenomics analysis identified 68 significantly differentially abundant functional pathways between US and MX. This study documented multiple structural, environmental, and demographic differences between homes in the US and MX that may contribute to significantly different microbial composition of dust observed in these two countries.

show abstract

Co-occurrence network analysis reveals the alterations of the skin microbiome and metabolome in adults with mild to moderate atopic dermatitis

Cited by 3 publications

References 59 publications

Microbiome and lipidomic analysis reveal the interplay between skin bacteria and lipids in a cohort study

Microbiome and lipidomic analysis reveal the interplay between skin bacteria and lipids in a cohort study

Staphylococcus aureus colonizing the skin microbiota of adults with severe atopic dermatitis exhibits genomic diversity and convergence in biofilm traits

Environmental and structural factors associated with bacterial diversity in household dust across the Arizona-Sonora border

Contact Info

Product

Resources

About