Multi-mycotoxin stable isotope dilution LC-MS/MS method for Fusarium toxins in cereals

Habler, Katharina; Rychlik, Michael

doi:10.1007/s00216-015-9110-7

Cited by 64 publications

(60 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, conjugated forms of DON (DON-3-Glc, 15ADON, 3ADON) were included [50]. Isotopically labeled standards of 3ADON, T2, enniatins, and beauvericin were prepared in-house.…”

Section: Lc-ms/ms With Limited Cleanup or Without Cleanupmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

125

View full text Add to dashboard Cite

Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

show abstract

“…Moreover, conjugated forms of DON (DON-3-Glc, 15ADON, 3ADON) were included [50]. Isotopically labeled standards of 3ADON, T2, enniatins, and beauvericin were prepared in-house.…”

Section: Lc-ms/ms With Limited Cleanup or Without Cleanupmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

125

View full text Add to dashboard Cite

show abstract

“…The following described validation of our new multi-mycotoxin stable isotope dilution LC-MS/MS method for Fusarium toxins was performed similar to the validation procedure Habler & Rychlik (2016), Liu & Rychlik (2013), and Hu & Rychlik (2012) published.…”

Section: Methods Validationmentioning

confidence: 99%

Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer

et al. 2017

Self Cite

View full text Add to dashboard Cite

A stable isotope dilution LC-MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1-5%, 2-8% and 72-117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.

show abstract

“…A possible approach to cope with the matrix effect is represented by stable isotope dilution assay (SIDA) [26]. Multi-mycotoxins methods applying this technique have been developed [27,28], also thanks to in-house synthesis of labeled isotopologue mycotoxin standards, including isotopologues of modified forms (i.e., DON3Glc, 3Ac-DON, 15Ac-DON) [29]. Authenticity and method performance have been demonstrated by analyzing naturally contaminated samples, such as malt, beer and maize [30].…”

Section: From Targeted Lc-ms/ms Determination To Untargeted Hr-ms mentioning

confidence: 99%

Recent Advances and Future Challenges in Modified Mycotoxin Analysis: Why HRMS Has Become a Key Instrument in Food Contaminant Research

Righetti

Paglia

Galaverna

et al. 2016

Toxins

View full text Add to dashboard Cite

Mycotoxins are secondary metabolites produced by pathogenic fungi in crops worldwide. These compounds can undergo modification in plants, leading to the formation of a large number of possible modified forms, whose toxicological relevance and occurrence in food and feed is still largely unexplored. The analysis of modified mycotoxins by liquid chromatography–mass spectrometry remains a challenge because of their chemical diversity, the large number of isomeric forms, and the lack of analytical standards. Here, the potential benefits of high-resolution and ion mobility mass spectrometry as a tool for separation and structure confirmation of modified mycotoxins have been investigated/reviewed.

show abstract

Multi-mycotoxin stable isotope dilution LC-MS/MS method for Fusarium toxins in cereals

Cited by 64 publications

References 26 publications

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer

Recent Advances and Future Challenges in Modified Mycotoxin Analysis: Why HRMS Has Become a Key Instrument in Food Contaminant Research

Contact Info

Product

Resources

About