Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer

Habler, Katharina; Gotthardt, Marina; Schüler, Jan; Rychlik, Michael

doi:10.1016/j.foodchem.2016.09.100

Cited by 49 publications

(32 citation statements)

References 26 publications

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“…The accuracy was confirmed by analysis of commercially available reference materials and samples from interlaboratory testing. This method was later applied to beer [71]. Although satisfactory recoveries were achieved, the LOQ of 20 μg/L for DON-3-Glc is too high for beer control, where common levels of DON-3-Glc are usually lower than 20 μg/L.…”

Section: Lc-ms/ms With Limited Cleanup or Without Cleanupmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

121

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Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

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Section: Lc-ms/ms With Limited Cleanup or Without Cleanupmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

121

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“…However, UHPLC requires reference compounds in order to identify the structures of unknown samples. Thus, tandem mass spectrometry (MS/MS) plays a key role in the chemical component analysis of TCM, has been integrated into UHPLC (Habler, Gotthardt, Schüler, & Rychlik, ; Zhang et al, ). Because the integrated UHPLC–MS/MS has many prominent advantages, such as high sensitivity and selectivity, short analytical time and low organic‐solvent consumption, attention has drown to its use in the analysis of multiple chemical components in TCMs with complex matrices (Ye, ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of eight components in Angelica sinensis based on UHPLC‐ESI‐MS/MS method for quality evaluation

Gui

Zheng²

2018

Biomedical Chromatography

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A method employing ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for determination of eight components including ferulic acid, senkyunolide A, butylphthalide, ligustilide, butylidenephalide, senkyunolide I, senkyunolide H and levistolide A in Angelica sinensis was established. The separation was carried out using a Waters ACQUITY UHPLC BEH C column with gradient elution with 0.1% formic acid aqueous and acetonitrile at a flow rate of 0.4 mL/min. Good linearity was attained with R of 0.9983-0.9998 in wide concentration ranges. The method had limit of detection (LOD) and quantitation (LOQ) in the range of 0.42-6.98 ng/mL and 1.39-23.28 ng/mL, respectively. Intra- and inter-day precisions varied with relative standard deviations (RSDs) from 0.33% to 0.88% and 0.37% to 1.04%, respectively. Moreover, the average recoveries were in a satisfactory range of 92.7%-102.1% with RSDs of less than 3.60%. Finally, the method was successfully applied to analyze 19 batches of A. sinensis samples grown in Min County, Gansu province, China, as well as that collected in other regions. The findings indicated that the established method is reliable and may thus be applied as a powerful tool for qualitative and quantitative analysis of components in A. sinensis, which has its implications in quality control of A. sinensis.

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“…It should be noted that removal of symptomatic kernels might not sufficiently reduce the total amount of fungal DNA and mycotoxins in individual malt samples. This is still crucial for ensuring the quality of malt for brewing, whereas multimycotoxin analyses in recent years did not reveal an acute risk for beer consumers (Habler et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Fusarium Species on Barley Malt: Is Visual Assessment an Appropriate Tool for Detection?

et al. 2017

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Cereal Chem. 94(4):659-669Fusarium infections in malting barley cause mycotoxin contamination, quality impairment, and processing difficulties. The visual assessment of barley malt is a commonly applied practice in the malting and brewing industry to screen cereal batches for fungal infection, because it assumes a direct connection between occurring symptomatology and actual fungal contamination. The exceedance of a defined limit of red kernels (usually five to seven) in a 200 g subsample of malt is associated with an unjustifiable risk for further processing and can lead to reductions in price or the rejection of the entire batch. The present study evaluated the suitability of this method to ensure product quality and safety. It was further intended to resolve the presumed linkage between kernel discoloration and Fusarium infection. In general, symptomatology showed low predictability for Fusarium contamination. However, significant correlations became apparent between the number of discolored kernels and fungal DNA contents under conditions of higher levels of infection, although this was not the case for mycotoxin levels. Although symptomatology is likely overinterpreted in regard to its reliability as an indicator for Fusarium contaminations, it might still assist in assessing the risk of certain fungal contaminants. † Corresponding

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Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer

Cited by 49 publications

References 26 publications

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Simultaneous determination of eight components in Angelica sinensis based on UHPLC‐ESI‐MS/MS method for quality evaluation

Fusarium Species on Barley Malt: Is Visual Assessment an Appropriate Tool for Detection?

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