Multi locus sequence type comparison of invasive and commensal Haemophilus influenzae isolates from Delhi

Saikia, KK; Bewal, Ramesh K.; Bansal, Devanshu; Kapil, Arti; Sood, Seema; Nk, Arora; Das, Balaram

doi:10.4103/0255-0857.81800

Cited by 2 publications

(2 citation statements)

References 16 publications

(10 reference statements)

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“…MLST has advantage in long-term global studies and for the assessment of evolutionary relationship among strains. MLST database has been generated by community efforts by submitting the data publically on the website [ 12 ] and is a valuable resource for long term epidemiology and population genetics analysis of pathogenic organisms [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Typhoidal Salmonellae: Use of Multi-Locus Sequence Typing to Determine Population Structure

et al. 2016

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Enteric fever is an invasive infection predominantly caused by Salmonella enterica serovars Typhi and Paratyphi A. The pathogens have evolved from other nontyphoidal salmonellaeto become invasive and host restricted. Emergence of antimicrobial resistance in typhoidal salmonellae in some countries is a major therapeutic concern as the travelers returning from endemic countries carry resistant strains to non endemic areas. In order to understand the epidemiology and to design disease control strategies molecular typing of the pathogen is very important. We performed Multilocus Sequence Typing (MLST) of 251 S. Typhi and 18 S. Paratyphi strains isolated from enteric fever patients from seven centers across India during 2010-2013to determine the population structure and prevalence of MLST sequence types in India. MLST analysis revealed the presence of five sequence types (STs) of typhoidal salmonellae in India namely ST1, ST2 and ST3 for S. Typhi and ST85 and ST129 for S. Paratyphi A.S. Typhi strains showed monophyletic lineage and clustered in to 3 Sequence Types—ST1, ST2 and ST3 and S. Paratyphi A isolates segregated in two sequence types ST85 and ST129 respectively. No association was found between antimicrobial susceptibility and sequence types. This study found ST1 as the most prevalent sequence type of S. Typhi in India followed by ST2, which is in concordance with previous studies and MLST database. In addition a rare sequence type ST3 has been found which is reported for the first time from the Indian subcontinent. Amongst S. Paratyphi A, the most common sequence type is ST129 as also reported from other parts of world. This distribution and prevalence suggest the common spread of the sequence types across the globe and these findings can help in understanding the disease distribution.

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Section: Introductionmentioning

confidence: 99%

Typhoidal Salmonellae: Use of Multi-Locus Sequence Typing to Determine Population Structure

et al. 2016

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“…H. influenzae is a major cause of meningitis, sepsis, otitis media, and other diseases, and H. influenzae type b is the most pathogenic ( Moxon and Vaughn, 1981 ; Saikia et al., 2011 ). The traditional culture method is the commonly used technique for detection of H. influenzae .…”

Section: Discussionmentioning

confidence: 99%

Establishment and Clinical Application of a RPA-LFS Assay for Detection of Capsulated and Non-Capsulated Haemophilus influenzae

Wang

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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A recombinase polymerase amplification-lateral flow strip assay was established for detection of the outer membrane protein P6 (omp6) and the capsule encoding gene bexA of Haemophilus influenzae and the detection limit, sensitivity, and specificity were determined. Specific primers and probes were designed based on the published nucleotide sequences of omp6 and bexA. The minimum detection limit was determined with standard strains and the practical applicability of the RPA-LFS assay was assessed by detection of 209 clinical samples. The results confirmed that the RPA-LFS assay was both specific and sensitive for the detection of capsulated and non-capsulated H. influenzae with a detection limit of 1 CFU/µL. The detection rate of the 209 clinical samples was 97.1%, while the detection rate of capsulated H. influenzae was 63.2%. The detection results were consistent with the traditional culture method and dual polymerase chain reaction (PCR), confirming the applicability of the RPA-LFS assay.

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Multi locus sequence type comparison of invasive and commensal Haemophilus influenzae isolates from Delhi

Cited by 2 publications

References 16 publications

Typhoidal Salmonellae: Use of Multi-Locus Sequence Typing to Determine Population Structure

Typhoidal Salmonellae: Use of Multi-Locus Sequence Typing to Determine Population Structure

Establishment and Clinical Application of a RPA-LFS Assay for Detection of Capsulated and Non-Capsulated Haemophilus influenzae

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