Multi-Glycomic Characterization of Fiber from AOAC Methods Defines the Carbohydrate Structures

Couture, Garret; Luthria, Devanand L.; Chen, Ye; Bacalzo, Nikita; Tareq, Fakir Shahidullah; Harnly, James; Phillips, Katherine M.; Pehrsson, Pamela R.; McKillop, Kyle; Fukagawa, Naomi K.; Lebrilla, Carlito B.

doi:10.1021/acs.jafc.2c06191

Cited by 5 publications

(9 citation statements)

References 47 publications

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“…Glucose, fructose, and sucrose are the most abundant free mono-and disaccharides in raw corn according to Garret Couture et al (2021) [24], which is confirmed by the data of our study.…”

Section: Discussion Of Research Resultssupporting

confidence: 92%

The study of carbohydrates of corn raw materials

Karpiuk

Kyslychenko

Hnoievyі³

et al. 2023

SR: PS

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The aim. The aim of the study was to study the content of polysaccharide fractions depending on the dissolution, qualitative composition and quantitative content of sugars in corn silk, leaves and roots. Materials and methods. To study different fractions of polysaccharides - water-soluble polysaccharides (WSPS), pectin substances (PS), hemicelluloses A (HC A) and B (HM B) used a gravimetric method based on the extraction of polysaccharide fractions with a suitable solvent followed by sedimentation and weighing of the sediment. Determination of the qualitative composition and quantitative content of monosaccharides in corn medicinal plant materials (MPM) was carried out by the method of gas chromatography-mass spectrometry (GC/MS). Results. Conducted studies of corn silk, leaves and roots carbohydrates using the fractionation method indicate the following trend of BAS accumulation in the studied raw materials: the content of WSPS and PS in corn silk exceeded the content of these compounds in other types of raw materials – 4.07±0.14 % and 7.20±0.29 %, respectively; leaves accumulated the most HC A and HC B compared to the content of these fractions in other samples - 6.81±0.21 % and 21.20±0.84 %, respectively. It was established by the GC/MS method that 4 substances were identified in corn silk in the free state: arabinose, glucose, galactose and fructose. After hydrolysis 5 substances were identified in corm silk - arabinose, xylose, mannose, glucose, galactose. Among the free sugars 3 compounds were found in corn leaves - glucose, fructose and sucrose. After hydrolysis, 4 compounds were identified in in corn leaves – arabinose, xylose, glucose and galactose. In corn roots 3 compounds are found in a free form - glucose, fructose and sucrose. After hydrolysis 4 compounds were identified in corn roots - arabinose, xylose, glucose and galactose. Conclusions. The obtained results indicate a significant content of polysaccharides and sugars in the raw materials of corn, which makes it possible to predict the anti-inflammatory, detoxifying, adsorbing, energetic activity of the studied types of corn plant raw materials

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“…Glucose, fructose, and sucrose are the most abundant free mono-and disaccharides in raw corn according to Garret Couture et al (2021) [24], which is confirmed by the data of our study.…”

Section: Discussion Of Research Resultssupporting

confidence: 92%

The study of carbohydrates of corn raw materials

Karpiuk

Kyslychenko

Hnoievyі³

et al. 2023

SR: PS

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“…We envision our approach as a sensitive, highly customizable tool accessible to the food science community. The methodology is also readily incorporated into the more comprehensive multiglycomics workflow we have previously developed . Such a tool can ultimately provide insight into the structure of food and its relationship to human health by facilitating a high throughput that would be practically not achievable using existing standard analytical methods.…”

Section: Resultsmentioning

confidence: 99%

Combined Alcohol Soluble Carbohydrate Determination (CASCADE) of Food

Ehlers Cheang,

Jiang,

Suarez

et al. 2024

ACS Food Sci. Technol.

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Alcohol soluble carbohydrates (ASCs) comprise one of the most abundant classes of dietary carbohydrates. While it is generally accepted that carbohydrates form a healthy part of the diet, the types and amounts consumed are a source of disagreement among practitioners. In particular, the role of ASCs, commonly referred to as free sugars, and comprising a range of mono-, di-, and oligosaccharides, is an active area of debate. These arguments likely arise from a dearth of chemical structures. Studies have implicated diets high in free glucose, fructose, and other rapidly digested ASCs as being associated with increases in cardiovascular and metabolic diseases, but this belies the full scope of monosaccharides, disaccharides, and oligosaccharides found in food. A growing body of evidence has highlighted the role of indigestible oligosaccharides that are part of "low molecular weight soluble fiber" in promoting human health through gut microbiome-mediated mechanisms. Despite their ubiquitous presence in food and relevance to health, there is a lack of comprehensive, rapid-throughput analytical tools for the absolute quantitation of ASCs. To address this critical gap, we propose a rapid-throughput, highly expandable platform for native Combined Alcohol Soluble CArbohydrate DEtermination (CASCADE) in food. To highlight the robustness of the method across complex matrices, CASCADE analysis was applied to commonly consumed foods including fermented and functionalized foods. The expandability of the platform was demonstrated by the inclusion of sugar alcohols in the analyte panel which are both naturally occurring and added to foods. Overall, we envision CASCADE as a useful tool for the nascent field of precision nutrition, which will necessitate detailed structures of foods.

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“…Currently, there are still many studies focusing on the simultaneous separation of different types of monosaccharides. A different devitization method coupled with LC-MS/MS [45][46][47], and GC-MS [48][49][50] was developed for the accurate analysis of monosaccharides. Furthermore, NMR was also developed for the identification of pentosan polysulfate monosaccharide composition [51].…”

Section: Evaluation Of Monosaccharide Composition Determination Methodsmentioning

confidence: 99%

Effects of Hydrolysis Condition and Detection Method on the Monosaccharide Composition Analysis of Polysaccharides from Natural Sources

Zhao,

Kuang,

Zhang

et al. 2023

Separations

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Monosaccharide composition analysis is essential to the structural characterization and research into the biological activity of polysaccharides. In this study, a systematic comparison was performed among commonly used monosaccharide composition analysis methods, including colorimetric and chromatographic methods. These were tested on 16 aldoses, ketoses, alditols, amino sugars, and uronic acids. Furthermore, the effect of hydrolysis methods was also investigated. The results showed that the phenol sulfuric acid method is greatly affected by the type of monosaccharide that is used as the reference substance. The determination of uronic acid using sulfuric acid carbazole is less affected by neutral sugars than that method using m-hydroxybiphenyl. The high-performance thin-layer chromatography (HPTLC) method can simultaneously analyze multiple samples and accurately determine the type of uronic acid. High-performance liquid chromatography (HPLC) can provide a good qualitative and quantitative analysis of aldose, amino sugars, and uronic acids, while gas chromatography–mass spectrometry (GC-MS) can detect aldose, ketose, and alditols. Fructose was detected in a large amount in inulin and Codonopsis pilosula after one-step hydrolysis, while it was totally destroyed in two-step hydrolysis. The release of galacturonic acid significantly increased after two-step hydrolysis in pectin and Lycium barbarum, which indicated that one-step hydrolysis is not enough for acidic polysaccharides. The results of this study are beneficial for selecting appropriate hydrolysis and analysis methods in order to accurately analyze the monosaccharide compositions of natural polysaccharides.

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Multi-Glycomic Characterization of Fiber from AOAC Methods Defines the Carbohydrate Structures

Cited by 5 publications

References 47 publications

The study of carbohydrates of corn raw materials

The study of carbohydrates of corn raw materials

Combined Alcohol Soluble Carbohydrate Determination (CASCADE) of Food

Effects of Hydrolysis Condition and Detection Method on the Monosaccharide Composition Analysis of Polysaccharides from Natural Sources

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