Multi-functional oxidase-like activity of praseodymia nanorods and nanoparticles

Jiang, Lei; Han, Yaning; Fernández-García, Susana; Tinoco, Miguel; Li, Zhuang; Nan, Pengli; Sun, Jingtao; Delgado, J. Juan; Hu, Pan; Blanco, Ginesa; López, Javier Martínez; Hungría, B. Ana; Calvino, J. Jose; Chen, Xiaowei

doi:10.1016/j.apsusc.2022.155502

Cited by 5 publications

(6 citation statements)

References 46 publications

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“…As for Pr 6 O 11 mimicking oxidase, it was found that F – has a strong inhibitory effect, while the other halides (Cl – and Br – ) and anions (SO 4 2– , PO 4 3– , and CO 3 2– ) do not induce such effects (Figure S5). The effects of pH and temperature on ABTS oxidation by Pr 6 O 11 NRs were also examined (Figure S6). The results suggest that the optimum pH is 4.0, and the catalytic activity increases with temperature at 10–50 °C.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Our previous studies have shown that Pr 6 O 11 NRs demonstrated excellent oxidase-like activity with good enzymatic performance and a unique hole + -mediated oxidation process. 23 This property makes it particularly suitable to replace natural enzymes on assays based on common substrates such as ABTS. On adding 0.1 mg/mL Pr 6 O 11 NRs to 0.1 mM ABTS, the colorless medium was gradually oxidized to a colored ABTS + solution (Figure 2a), in agreement with previous results.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Meanwhile, amounts of free phosphate ions, a byproduct of NTP hydrolysis, were quantified after incubation of NTP with Pr 6 O 11 NRs (Figure ) do not induce such effects (Figure S5). 23 The effects of pH and temperature on ABTS oxidation by Pr 6 O 11 NRs were also examined (Figure S6). The results suggest that the optimum pH is 4.0, and the catalytic activity increases with temperature at 10−50 °C.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Enzyme-Free Colorimetric Method for Fast Detection of PIK3CA Gene Mutation by Praseodymia Nanorods

Jiang

Wang

et al. 2023

Anal. Chem.

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The frequently mutated phosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA) gene is associated with multiple tumors and endocytosis of viruses. Identification of muted nucleotides at the hotspot can help in finding the susceptible people who are vulnerable to cancers and viruses. Herein, a simple enzyme-free colorimetric method is developed for the quick detection of PIK3CA gene mutations. The main mechanism lies in the dissimilar interactions between praseodymia nanorods and different nucleotides, as well as the underlying oxidase-mimicking characteristics of praseodymia. With rational designs of probes and processes, this method has great potential for expanded applications in the screening of mutations in other genes of interest.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Enzyme-Free Colorimetric Method for Fast Detection of PIK3CA Gene Mutation by Praseodymia Nanorods

Jiang

Wang

et al. 2023

Anal. Chem.

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“…In order to accomplish this, five different radical scavengers were added during the typical dopamine oxidation reaction with MNWs, to capture specific ROS released during the reaction (Figure S4). The presence of hydroxyl radicals (•OH) was detected using isopropyl alcohol (IA) and methanol (MA), the existence of superoxide anion radicals (O2 •− ) was detected using benzoquinone (BQ) and superoxide dismutase (SOD), and singlet oxygen ( 1 O2) was detected using sodium azide (SA) [31]. According to the experimental findings, O2 •− and 1 O2…”

Section: Investigation Of the Dopamine Oxidase-like Activity Of Mnwsmentioning

confidence: 98%

Highly Crystalline Oxidase-like MnOOH Nanowire-Incorporated Paper Dipstick for One-Step Colorimetric Detection of Dopamine

2023

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Developing a convenient detection method for dopamine holds a significant incentive due to its high clinical significance. Herein, we synthesize crystalline MnOOH nanowires (MNWs) via a simple solvothermal treatment of KMnO4 and demonstrate that they possess excellent oxidase-like activity owing to the presence of pure Mn3+ sites on the MNWs. Particularly, MNWs catalyze the rapid oxidation of dopamine into aminochromes, which show a vivid brown color. The dopamine oxidase-like activity of MNWs follows the typical Michaelis–Mentenkinetics with excellent storage stability. Based on the affirmative catalytic features, a paper dipstick incorporating MNWs in the detection zone is constructed for the one-step colorimetric detection of target dopamine. By immersing the dipstick into the sample solution for 30 min, the sample spontaneously moves to the detection zone due to capillary force, yielding a brown color proportional to the amount of dopamine, which is quantified from an image acquired using a smartphone. With the MNW-containing solution-based assay and MNW-incorporated paper dipstick, dopamine is successfully determined with high selectivity, sensitivity, and detection precision when using spiked human serum and pharmaceutical dopamine injection samples, respectively. Successful analytical values such as the dynamic linear ranges of 3–60 μM and 0.05–7 mM are achieved with the solution-based assay and paper dipstick, respectively, along with excellent detection accuracy (95–99%) and precision (1.0–3.1%). Hence, we developed a simple and efficient nanozyme-based paper dipstick biosensor for dopamine that can be used in point-of-care testing environments.

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Synergistic (Ce/Pr/Nd)3+-doped TiO2 hetero-system as bifunctional catalyst, electrode material, and electron conveyance layer in perovskite solar cells

Jaffri,

Ahmad,

Abrahams

et al. 2024

International Journal of Hydrogen Energy

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Multi-functional oxidase-like activity of praseodymia nanorods and nanoparticles

Cited by 5 publications

References 46 publications

Enzyme-Free Colorimetric Method for Fast Detection of PIK3CA Gene Mutation by Praseodymia Nanorods

Enzyme-Free Colorimetric Method for Fast Detection of PIK3CA Gene Mutation by Praseodymia Nanorods

Highly Crystalline Oxidase-like MnOOH Nanowire-Incorporated Paper Dipstick for One-Step Colorimetric Detection of Dopamine

Synergistic (Ce/Pr/Nd)3+-doped TiO2 hetero-system as bifunctional catalyst, electrode material, and electron conveyance layer in perovskite solar cells

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