Multi-epitope recombinant vaccine induces immunoprotection against mixed infection of Eimeria spp.

Ding, Junjun; Qian, Weifeng; Li, Qun; Liu, Qiaorong

doi:10.1007/s00436-011-2764-y

Cited by 18 publications

(10 citation statements)

References 38 publications

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“…Many publications have focused on DNA vaccination against Eimeria in chickens [2,4,8,[15][16][17][56][57][58][59][60][61][62][63][64][65][66] (Table 2). Specific immune responses and/or relatively high levels of clinical protection were obtained against this protozoan (attenuation of body weight loss, lesion scores and oocysts ratios upon Eimeria experimental challenge).…”

Section: Different Poultry Dna Vaccination Modelsmentioning

confidence: 99%

“…The authors first inoculated poultry with the DNA vaccine. The subsequent booster inoculation either used a recombinant protein corresponding to the protein encoded by the DNA vaccine, or a recombinant fowlpox virus expressing this antigen [8,23,35,77,80]. The DNA prime-recombinant protein regimen was particularly efficient for an IBDV model where 100% of the chickens were protected (VP2 antigen) [35].…”

Section: Heterologous Prime-boost Strategiesmentioning

confidence: 99%

“…The DNA prime-recombinant protein regimen was particularly efficient for an IBDV model where 100% of the chickens were protected (VP2 antigen) [35]. It also induced high protective immunity against Eimeria infection [8] and avian influenza [23] in chickens. In turkeys, this regimen gave some protection against Chlamydophila psittaci infections [95].…”

Section: Heterologous Prime-boost Strategiesmentioning

confidence: 99%

“…First, some conventional inactivated or attenuated vaccines are difficult to produce, store or distribute widely [1][2][3] or are costly [2,4]. Some of these conventional vaccines are not fully efficient due, for example, to the presence of maternal antibodies in young birds [5,6] or to the existence of multiple pathogenic strains and/or the emergence of new strains [7][8][9][10]. Furthermore, there is a risk with some conventional vaccines of reversion to virulence in certain cases [3,11] or of adverse reactions, especially in young birds [2,12,13].…”

Section: Introductionmentioning

confidence: 99%

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DNA vaccination of poultry: The current status in 2015

Meunier

Chemaly

Dory

2016

Vaccine

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DNA vaccination is a promising alternative strategy for developing new human and animal vaccines. The massive efforts made these past 25 years to increase the immunizing potential of this kind of vaccine are still ongoing. A relatively small number of studies concerning poultry have been published. Even though there is a need for new poultry vaccines, five parameters must nevertheless be taken into account for their development: the vaccine has to be very effective, safe, inexpensive, suitable for mass vaccination and able to induce immune responses in the presence of maternal antibodies (when appropriate). DNA vaccination should meet these requirements. This review describes studies in this field performed exclusively on birds (chickens, ducks and turkeys). No evaluations of avian DNA vaccine efficacy performed on mice as preliminary tests have been taken into consideration. The review first describes the state of the art for DNA vaccination in poultry: pathogens targeted, plasmids used and different routes of vaccine administration. Second, it presents strategies designed to improve DNA vaccine efficacy: influence of the route of administration, plasmid dose and age of birds on their first inoculation; increasing plasmid uptake by host cells; addition of immunomodulators; optimization of plasmid backbones and codon usage; association of vaccine antigens and finally, heterologous prime-boost regimens. The final part will indicate additional properties of DNA vaccines in poultry: fate of the plasmids upon inoculation, immunological considerations and the use of DNA vaccines for purposes other than preventing infectious diseases.

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Section: Different Poultry Dna Vaccination Modelsmentioning

confidence: 99%

Section: Heterologous Prime-boost Strategiesmentioning

confidence: 99%

Section: Heterologous Prime-boost Strategiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

DNA vaccination of poultry: The current status in 2015

Meunier

Chemaly

Dory

2016

Vaccine

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show abstract

“…11,[18][19][20][21] The research and development of these vaccines also relies on an accurate and sensitive way of determining the identity and purity of Eimeria strains used. For basic research, the incursion of contaminating Eimeria parasites in presumably purified strains can lead to invalid interpretation of data.…”

Section: Introductionmentioning

confidence: 99%

Designing strategies for the control of coccidiosis in chickens on poultry farms using modern diagnostic tools

Frölich¹,

Farhat²,

Wallach³

2013

RIP

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Abstract:Coccidiosis is caused by the intracellular protozoan parasite Eimeria and is a major worldwide problem in the poultry industry today. The current strategies to control the disease still rely heavily on anticoccidial drugs and on the use of shuttle programs to periodically introduce attenuated live parasites onto poultry farms. Recently, because of the improved performance of broiler chickens, farmers are able to raise chickens for market within a period of only 5-6 weeks. This short growth period enables the development of strategies for coccidiosis control either by ensuring an even exposure of chickens to parasites in the litter, inducing immunity against reinfection, or by keeping parasite numbers below a threshold level wherein only subclinical infections will occur. Therefore, the development and use of new diagnostic tools to precisely identify the Eimeria species and strain and to measure the number of oocysts in the feces and litter can be very helpful in predicting exposure and designing strategies for the control of coccidiosis in the field. The purpose of this study was to describe recently developed methods of diagnosis in terms of their sensitivity and usefulness, describe ways in which litter oocyst numbers can be quantitated, and start to develop an analytical tool that can be used for designing strategies for the control of coccidiosis. A multiplex polymerase chain reaction-based assay has been developed that enables the identification of all seven Eimeria species in a single reaction tube. Previous reports have shown it is possible to detect one oocyst using a polymerase chain reaction-based assay; however, other reports have found that under both laboratory and field conditions the minimal level of sensitivity is only 20 oocysts. In addition, the present authors' studies have found that when using spiked mixed samples, the level of detection of a contaminating Eimeria species is only 5%. Therefore, it is imperative that a new method be developed with a tenfold higher level of sensitivity for field-testing. Once this test is developed and validated, it can be used to monitor the litter and assess the parameters (climate, hygiene, breed of chicken, etc) that govern coccidial outbreaks on farms.

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Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities

et al. 2022

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Chicken coccidiosis is an economically significant disease of commercial chicken industry accounting for losses of more than £10.4 billion (according to 2016 prices). Additionally, the costs incurred in prophylaxis and therapeutics against chicken coccidiosis in developing countries (for instance Pakistan according to 2018 prices) reached US $45,000.00 while production losses for various categories of chicken ranges 104.74 to US $2,750,779.00. The infection has been reported from all types of commercial chickens (broiler, layer, breeder) having a range of reported prevalence of 7–90%. The concern of resistance towards major anticoccidials has provided a way forward to vaccine research and development. For prophylaxis of chicken coccidiosis, live virulent, attenuated, ionophore tolerant strains and recombinant vaccines have been extensively trialed and commercialized. Eimeria antigens and novel vaccine adjuvants have elicited the protective efficacy against coccidial challenge. The cost of production and achieving robust immune responses in birds are major challenges for commercial vaccine production. In the future, research should be focused on the development of multivalent anticoccidial vaccines for commercial poultry. Efforts should also be made on the discovery of novel antigens for incorporation into vaccine designs which might be more effective against multiple Eimeria species. This review presents a recap to the overall progress against chicken Eimeria with particular reference to previous decade. The article presents critical analysis of potential areas for future research in chicken Eimeria vaccine development.

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Multi-epitope recombinant vaccine induces immunoprotection against mixed infection of Eimeria spp.

Cited by 18 publications

References 38 publications

DNA vaccination of poultry: The current status in 2015

DNA vaccination of poultry: The current status in 2015

Designing strategies for the control of coccidiosis in chickens on poultry farms using modern diagnostic tools

Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities

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