Multi-drug resistant Vibrio species isolated from abattoir effluents in Nigeria

Abstract

Introduction: The antibiograms of Vibrio species isolated from abattoir effluents in the Niger Delta region of Nigeria were investigated with respect to their public health significance. Methodology: Vibrio species were isolated and identified using standard microbiological and molecular techniques, while antibiogram of isolates was tested and interpreted using the disk diffusion method described by the Clinical and Laboratory Standards Institute. Results: Of 150 presumptive isolates, 48 (32%) were confirmed to be Vibrio spp. by PCR; of these, 23 (47.9%) were V. cholerae, 11 (22.9%) were V. fluvialis, 8 (16.7%) were V. vulnificus, and 6 (12.5%) were V. parahaemolyticus. The antibiogram revealed that Vibrio species were generally resistant to ampicillin (60%-67%), trimethoprim (80%-100%), and tetracycline (60%-83%), whereas they were sensitive to ceftriaxone (86%-100%), the aminoglycosides (67%-100%), imipenem (86%-100%), ofloxacin (83%-100%), and chloramphenicol (67%-100%). The isolates exhibited multiple antibiotic resistance (MAR) with an average MAR index of 0.23. Conclusions: This study demonstrated that abattoir effluents are important reservoirs for multidrug-resistant Vibrio species that might be considerable contributors to the recurrent episodes of epidemic cholera and non-Vibrio cholera infections in Nigeria.

Multiple Antibiotic Resistant Vibrio Pathotypes with the Incidence of V. Cholerae and V. parahaemolyticus in Fish and Fish Storage Water in Okitipupa and Igbokoda Areas, Nigeria

“…Resistance observed against tetracycline also corroborate the findings of Haenen et al [13] who reported a sudden acquired multiple antibiotics resistance in Vibrio species. Other researchers also reported tetracycline resistant in vibrio species obtained from diverse sources of water such as wastewater effluent [20], abbatoir effluent [31] and surface water [16].…”

Resistance of Vibrio cholera to antibiotics that inhibit cell wall synthesis: A systematic review and meta-analysis

“…(1998), Jagadeeshan et al (2009), Kiiru et al (2013), Kumar and Lalitha (2013), Li et al (2011), Mandal et al (2010), Mercy et al (2014), Mishra et al (2011), Mohapatra et al (2007), Odjadjare and Igbinosa (2017), Onyuka et al (2011), Osawa et al (2020), Ottaviani et al (2018), Prabhu et al (2007), Radu et al (2002), Kutar et al (2013), Singh et al (2018), Song et al (2013), Wang et al (2016), Waturangi et al (2013), Zachariah et al (2002), Kumaran and Citarasu (2016)].…”

Distribution and Antibiotic Susceptibility Profiles of Vibrio Species Isolated from Selected Abattoirs in Akoko South-West, Ondo State, Nigeria

“…According to [5], abattoir effluents are important reservoirs of multidrug resistant Vibrio pathogens. This implies that abattoir effluents could be important contributors to the recurrent episodes of epidemic cholera and non-Vibrio cholerae outbreaks in Nigeria.…”

“…Abattoir inspection of live animals (ante-mortem) and carcass (post-mortem) are critical to surveillance for animal diseases and zoonoses [4]. [5] reported that pathogens of zoonotic importance are associated with more than 80% of public abattoirs in Nigeria. This observation has serious public health implication as many Nigerian abattoirs dispose their effluents directly into streams and rivers without any form of treatment.…”