Multi drug resistance and -lactamase production by Klebsiella pneumoniae

Khadri, Habeeb; Surekha, S.; Lakshmi, S.; Narasimha, G.

doi:10.5897/ajb2007.000-2264

Cited by 3 publications

(4 citation statements)

References 13 publications

(15 reference statements)

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“…In addition, ESBL-producing strains of E. coli and K. The detection of ESBL enzymes is very important, because according to CLSI M100-S17 and M100-S18 (25,27) criteria, these organisms should be reported as resistant to all (12,16,25,39).…”

Section: Methodsmentioning

confidence: 99%

“…ESBLs originate from punctual genetic mutations in the active sites of the TEM-1, TEM-2, and SHV-1 enzymes (3,10,12,31,19,23,26,34), which enable the hydrolysis of extended-spectrum cephalosporins, monobactams, penicillins. (2,3,5,9,11,12,27,30). However, these plasmid mediated enzymes have their activity inhibited by clavulanic acid, tazobactam and sulbactam.…”

Section: Introductionmentioning

confidence: 99%

“…However, these plasmid mediated enzymes have their activity inhibited by clavulanic acid, tazobactam and sulbactam. Another important epidemiological characteristic is the possibility of these enzymes being transferred to other species via plasmids or transposons (2,3,5,6,9,11,12,21,27,28,30,34).…”

Section: Introductionmentioning

confidence: 99%

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Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus, State of Amazonas, Brazil

Ferreira¹,

Ferreira²,

Almeida³

et al. 2011

Braz. J. Microbiol.

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Antibiotic therapy in hematologic patients, often weak and susceptible to a wide range of infections, particularly nosocomial infections derived from long hospitalization periods, is a challenging issue. This paper presents ESBL-producing strains isolated from such hematologic patients treated at the Amazon Hematology and Hemotherapy Foundation (HEMOAM) in the Brazilian Amazon Region to identify the ESBL genes carried by them as well as the susceptibility to 11 antimicrobial agents using the E-test method. A total of 146 clinical samples were obtained from July 2007 to August 2008, when 17 gram-negative strains were isolated in our institution. The most frequent isolates confirmed by biochemical tests and 16S rRNA sequencing were E. coli (8/17), Serratia spp. (3/17) and B.cepacia (2/17). All gram-negative strains were tested for extended-spectrum-beta-lactamases (ESBLs), where: (12/17) strains carried ESBL; among these, (8/12) isolates carried blaTEM, blaCTX-M, blaOXA, blaSHV genes, (1/12) blaTEM gene and (3/12) blaTEM, blaCTX-M, blaOXA genes. Antibiotic resistance was found in (15/17) of the isolates for tetracycline, (12/17) for ciprofloxacin, (1/17) resistance for cefoxitin and chloramphenicol, (1/17) for amikacin and (3/17) cefepime. This research showed the presence of gram-negative ESBL-producing bacteria infecting hematologic patients in HEMOAM. These strains carried the blaTEM, blaSHV, blaCTX-M and blaOXA genes and were resistant to different antibiotics used in the treatment. This finding was based on a period of 13 months, during which clinical samples from specific populations were obtained. Therefore, caution is required when generalizing the results that must be based on posological orientations and new breakpoints for disk diffusion and microdilution published by CLSI 2010

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus, State of Amazonas, Brazil

Ferreira¹,

Ferreira²,

Almeida³

et al. 2011

Braz. J. Microbiol.

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“…The prevalence of Antimicrobial resistance (AMR) by K. pneumoniae approaches 50% in some countries, with particularly high rates in Eastern Europe and Latin America [4]. The incidence of ESBL producing strains among clinical Klebsiella isolates has steadily increased over the years and account for 6 to 17% of all nosocomial urinary tract infections [23].…”

Section: Risk Factors Prevalence and Antimicrobial Resistancementioning

confidence: 99%

Klebsiella pneumoniae and Its Growing Concern in Healthcare Settings

Woldu¹

2016

Clin Exp Pharmacol

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The increasing clinical incidence of antibiotic-resistant bacteria is a major global health care issue. Among MDR pathogens, Klebsiella pneumoniae (KP) is one of the world's most dangerous superbugs; and becoming resistant to virtually every antibiotic available today. Published articles cited majorly from three sources were browsed using selected keywords. Screening technique was done based on article relevance, topic match and English language match. Duplication of findings was avoided. Prevalence of KP among isolates was determined in different study places and the most alarming figure was seen in study conducted in Nigeria (64.2%) followed by India (33.9%) and Denmark (17.4%). Based on our pooled data from a number of studies conducted at different part of the globe, antibiotic resistances among KP isolates were found to be 100% for Cephradin, 87.5% for Cefeclor, 84% for Tobramycin, 82.5% for Cefotaxime, and 80.4% for Norfloxacin. Whereas, K. pneumonia was found to be more sensitive to Impenem (92.5%), Meropenem (92.5%), Amoxicillin/Clavulanic acid (87.5%), Gatifloxacin (85%), Moxifloxacin (75%); and chloraphenicol (62.8%). Carbapenems have been regarded as the treatment of choice for serious infections caused by extended-spectrum βeta-lactamase (ESBL)-producers. The combination therapy of fosfomycin+colistin demonstrated synergistic and bactericidal effect against one metallobetalactamase producing KP strains that were resistant to fosfomycin. Some of the factors that are responsible for the decline in number of newly discovered antibiotic include reduced financial grant from funding agencies, closure of many major pharmaceutical and large biotech companies; and the lack of financial reward for large pharmaceutical companies to develop new ambitious projects. In conclusion, MDR bacteria are emerging worldwide causing many public health problems and challenges to healthcare. Based on the result from our pooled data, we can conclude that currently we have only few antibiotics that are effective to treat KP.

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Mode of Antibacterial Activity of Eclalbasaponin Isolated from Eclipta alba

Ray

Bharali

Konwar

2013

Appl Biochem Biotechnol

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The present study was undertaken to evaluate the mode of antibacterial activity of Eclalbasaponin isolated from Eclipta alba, against selected Gram-positive and Gram-negative bacteria. The probable chemical structure was determined by using various spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR) and mass spectroscopy. The antibacterial activity was evaluated by well diffusion technique, pH sensitivity, chemotaxis, and crystal violet assays. Eclalbasaponin showed clear zone of inhibition against both Bacillus subtilis and Pseudomonas aeruginosa and exhibited growth inhibition at the pH range of 5.5-9.0. The isolated saponin exhibited its positive chemoattractant property for both bacterial strains. Results of crystal violet assay and the presence of UV-sensitive materials in the cell-free supernatant confirmed the cellular damages caused by the treatment of Eclalbasaponin. The release of intracellular proteins due to the membrane damage was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Changes in the cell surface structure and membrane disruption were further revealed by FTIR and scanning electron microscopy analysis. The present study suggests that the isolated saponin from E. alba causes the disruption of the bacterial cell membrane which leads to the loss of bacterial cell viability.

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Multi drug resistance and -lactamase production by Klebsiella pneumoniae

Cited by 3 publications

References 13 publications

Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus, State of Amazonas, Brazil

Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus, State of Amazonas, Brazil

Klebsiella pneumoniae and Its Growing Concern in Healthcare Settings

Mode of Antibacterial Activity of Eclalbasaponin Isolated from Eclipta alba

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