Multi-component thermostable cellulolytic enzyme production by Aspergillus niger HN-1 using pea pod waste: Appraisal of hydrolytic potential with lignocellulosic biomass

Sharma, Renu; Rawat, Rekha; Bhogal, Ravinder Singh; Oberoi, Harinder Singh

doi:10.1016/j.procbio.2015.01.025

Cited by 29 publications

(14 citation statements)

References 29 publications

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“…More so, various agricultural wastes including rice straw, wheat straw, sawdust, pea pods, etc. have been valorized for production of different lignocellulolytic enzymes in previous studies . The utilization of agrowastes by R. ornithinolytica OKOH‐1 for peroxidase production under SSF is economically viable because agricultural residues are cheaper alternatives to synthetic carbon sources such as kraft lignin; they are abundant and readily available in the environment .…”

Section: Resultsmentioning

confidence: 99%

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

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Mabinya

Okoh

et al. 2018

Biotech and App Biochem

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The industrial applications and prospects of microbial peroxidase are on the upwards trend, thus necessitating the search for sources with high turnaround time. Actinobacterial species have been a major source of peroxidase for the obvious reasons of having robust metabolite expression capabilities. However, other bacteria species have been underexplored for peroxidase production, hence the motivation for the investigation into the peroxidase production potential of Raoultella ornithinolytica OKOH-1 (KX640917). The bacteria expressed optimum specific peroxidase activity of 16.48 ± 0.89 U mg −1 , which is higher than those previously reported. The optimal fermentation conditions were pH 5 (3.44 ± 0.64 U mL −1 ), incubation temperature of 35 • C (5.25 ± 0.00 U mL −1 ), and agitation speed of 150 rpm (9.45 ± 2.57 U mL −1 ), with guaiacol and ammonium chloride as the best inducer and nitrogen supplement, respectively. On valorization of agrowastes as a sole carbon source for the secretion of peroxidase, sawdust gave the best peroxidase yield (15.21 ± 2.48 U mg −1 ) under solid-state fermentation. Also, a nonperoxide-dependent enzyme activity, which suggests probable laccase activity, was observed. The ability of the bacteria to utilize agrowastes is highly economical and as well a suitable waste management strategy. Consequently, R. ornithinolytica OKOH-1 is a promising industrial strain with dexterity for enhanced peroxidase production.

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Section: Resultsmentioning

confidence: 99%

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Falade

Mabinya

Okoh

et al. 2018

Biotech and App Biochem

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“…The abundance, availability and renewable nature of lignocellulosic materials confer on them the status of perfect candidates of cheap carbon sources. Agro-residues including sawdust, wheat straw, corn cobs, rice straw, peapods etc have been utilized for production of various lignocellulolytic enzymes through solid state and submerged fermentation processes [25,[57][58][59][60]. However, SSF is perhaps, more promising for optimum utilization of agricultural residues for enzyme production as SSF is reported to be economical [61].…”

Section: Assessment Of Agricultural Residues For Exoperoxidase Producmentioning

confidence: 99%

Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens

et al. 2019

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The increased industrial application potentials of peroxidase have led to high market demand, which has outweighed the commercially available peroxidases. Hence, the need for alternative and efficient peroxidase-producers is imperative. This study reported the process parameters for enhanced exoperoxidase production by Ensifer adhaerens NWODO-2 (accession number: KX640918) for the first time, and characterized the enzyme using molecular methods. Peroxidase production by the bacteria was optimal at 48 h, with specific productivity of 12.76 U mg−1 at pH 7, 30 °C and 100 rpm in an alkali lignin fermentation medium supplemented with guaiacol as the most effective inducer and ammonium sulphate as the best inorganic nitrogen source. Upon assessment of some agricultural residues as sources of carbon for the enzyme production, sawdust gave the highest peroxidase productivity (37.50 U mg−1) under solid-state fermentation. A search of the polymerase chain reaction (PCR)-amplified peroxidase gene in UniProtKB using blastx showed 70.5% similarity to an uncharacterized protein in Ensifer adhaerens but phylogenetic analysis suggests that the gene may encode a catalase-peroxidase with an estimated molecular weight of approximately 31 kDa and isoelectric point of about 11. The nucleotide sequence of the detected gene was deposited in the GenBank under the accession number MF374336. In conclusion, the ability of the strain to utilize lignocellulosic materials for peroxidase production augurs well for biotechnological application as this would greatly reduce cost, which is a major challenge in industrial enzyme production.

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“…Generally,vegetable processing units generate more than 30% (w/w) waste during harvesting, processing, and marketing. Based on vegetable production statistics, it has been noted that India is leading producer of green pea (Pisum sativum) with annual production of around 3.56 million metric tons, which subsequently generates more than 1 million ton of pea pod waste every year [9]. In day-to-day life, a huge amount of vegetable waste being generated is either ploughed back into soil or left as a city waste on a dumping ground, which ultimately causes environmental pollution [10].…”

Section: Introductionmentioning

confidence: 99%

Biobutanol production using pea pod waste as substrate: Impact of drying on saccharification and fermentation

et al. 2018

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PDF/A is an ISO-standardized version of the Portable Document Format (PDF) specialized for the digital preservation of electronic documents. The extension in the file name is pdf. PDF/A differs from "normal" PDF in that features ill-suited for long-term archiving are omitted. PDF/A embeds all fonts used in the document within the PDF file, so that the user of your file will not have to have the same fonts that you used to create the file installed on their computer in order to read it. Recommended versions are PDF/A-1a, -1b, -2a or -2b.PDF/A conversion -how to convert files to the PDF/A -format using different tools Microsoft Word (Office) and PDF-Xchange pro/Editor are installed on all Windows workstations managed by Aalto IT.

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Multi-component thermostable cellulolytic enzyme production by Aspergillus niger HN-1 using pea pod waste: Appraisal of hydrolytic potential with lignocellulosic biomass

Cited by 29 publications

References 29 publications

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens

Biobutanol production using pea pod waste as substrate: Impact of drying on saccharification and fermentation

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