Multi-colour fluorescence imaging of photosynthetic activity and plant stress

Lichtenthaler, H. K.

doi:10.32615/ps.2021.020

Cited by 24 publications

(18 citation statements)

References 56 publications

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“…Chl fluorescence is also used to detect and characterize abiotic and biotic stress responses (Guidi and Degl'Innocenti, 2012) and for phenotyping during breeding/selection of new stress resistant and/or higher yielding crops or microalgae (Chaerle et al, 2009; Pérez‐Bueno et al, 2019). It has also been extended to cover spatial resolution by fluorescence imaging (Johnson et al, 2009; Nedbal and Whitmarsh, 2004) which – depending on the resolution achieved ‐ allows to differentiate between macroscopic and/or microscopic variations in the fluorescence of a leaf related to differences in Chl responses, stress conditions (Lichtenthaler, 2021; Moustakas et al, 2021) or to variations in chloroplast structure at the subcellular level (Wientjes et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

ChloroSpec: A new in vivo chlorophyll fluorescence spectrometer for simultaneous wavelength‐ and time‐resolved detection

Nanda,

Shutova,

Cainzos

et al. 2024

Physiologia Plantarum

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Chlorophyll fluorescence is a ubiquitous tool in basic and applied plant science research. Various standard commercial instruments are available for characterization of photosynthetic material like leaves or microalgae, most of which integrate the overall fluorescence signals above a certain cut‐off wavelength. However, wavelength‐resolved (fluorescence signals appearing at different wavelengths having different time dependent decay) signals contain vast information required to decompose complex signals and processes into their underlying components that can untangle the photo‐physiological process of photosynthesis. Hence, to address this we describe an advanced chlorophyll fluorescence spectrometer ‐ ChloroSpec ‐ allowing three‐dimensional simultaneous detection of fluorescence intensities at different wavelengths in a time‐resolved manner. We demonstrate for a variety of typical examples that most of the generally used fluorescence parameters are strongly wavelength dependent. This indicates a pronounced heterogeneity and a highly dynamic nature of the thylakoid and the photosynthetic apparatus under actinic illumination. Furthermore, we provide examples of advanced global analysis procedures integrating this three‐dimensional signal and relevant information extracted from them that relate to the physiological properties of the organism. This conveniently obtained broad range of data can make ChloroSpec a new standard tool in photosynthesis research.

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Section: Introductionmentioning

confidence: 99%

ChloroSpec: A new in vivo chlorophyll fluorescence spectrometer for simultaneous wavelength‐ and time‐resolved detection

Nanda,

Shutova,

Cainzos

et al. 2024

Physiologia Plantarum

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“…Overgrazing can threaten the conservation of ecosystems through excessive defoliation or trampling of vegetation. It also indirectly increases the effect on soil structure (8,9). This leads to the acceleration of desertification processes (10).…”

Section: Introductionmentioning

confidence: 99%

Changes of photosynthetic pigments of Artemisia diffusa under the influence of grazing stress of livestock grazing in Karnabchul semi-desert, Uzbekistan

et al. 2023

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On the transformed natural rangelands of Karnabchul desert, the natural Artemisia diffusa studied four different grazing intensities the change in the amount of photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids). It was found that the amount of A. diffusa pigments also increased with increasing stress levels in livestock grazing. In general, different grazing intensities had a different impact on the morphological and physiological traits of plants, especially their photosynthetic pigments.

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“…Additionally, a previous study reported that the exposure of Arabidopsis to salt stress resulted in a decline in chlorophyll content [29], which also could be a result of the increase of F460 and F520 since the reabsorption of blue-green fluorescence is reduced [39]. The chlorophyll-fluorescence emission spectra usually exhibits two emission maxima around 690 nm and 740 nm, which are termed F690 and F740 [40,41]. The decrease of F690 is partially caused by the in vivo chlorophyll (overlapping of absorption and fluorescence emission bands of chlorophyll a forms) [42,43].…”

Section: Effect Of Salt Stress On Basic Fluorescence Parameters Of Arabidopsis Leavesmentioning

confidence: 99%

Multicolor Fluorescence Imaging for the Early Detection of Salt Stress in Arabidopsis

Tian

Xie

et al. 2021

Agronomy

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Salt stress is one of the abiotic factors that causes adverse effects in plants and there is an urgent need to detect salt stress in plants as early as possible. Multicolor fluorescence imaging, as a powerful tool in plant phenotyping, can provide information about primary and secondary metabolism in plants to detect the responses of the plants exposed to stress in the early stage. The purpose of this study was to evaluate the potential of multicolor fluorescence imaging’s application in the early detection of salt stress in plants. In this study, the measurements were conducted on Arabidopsis and the multicolor fluorescence images were acquired at 440, 520, 690, and 740 nm with a self-developed imaging system consisting of a UV light-emitting diode (LED) panel for an excitation at 365 nm, a charge coupled device (CCD) camera, interference filters, and a computer. We developed a classification method using the imaging analysis of multicolor fluorescence based on principal component analysis (PCA) and a support vector machine (SVM). The results showed that the four principal fluorescence feature combinations were the ideal indicators as the inputs of the SVM model, and the classification accuracies of the control and salt-stress treatment at 5 days and 9 days were 92.65% and 98.53%, respectively. The results indicated that multicolor fluorescence imaging combined with PCA and SVM could act as a tool for early detection in salt-stressed plants.

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Multi-colour fluorescence imaging of photosynthetic activity and plant stress

Cited by 24 publications

References 56 publications

ChloroSpec: A new in vivo chlorophyll fluorescence spectrometer for simultaneous wavelength‐ and time‐resolved detection

ChloroSpec: A new in vivo chlorophyll fluorescence spectrometer for simultaneous wavelength‐ and time‐resolved detection

Changes of photosynthetic pigments of Artemisia diffusa under the influence of grazing stress of livestock grazing in Karnabchul semi-desert, Uzbekistan

Multicolor Fluorescence Imaging for the Early Detection of Salt Stress in Arabidopsis

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