<i><scp>ChloroSpec</scp></i>: A new <i>in vivo</i> chlorophyll fluorescence spectrometer for simultaneous wavelength‐ and time‐resolved detection

Nanda, Sanchali; Shutova, Tatyana; Cainzos, Maximiliano; Hu, Chen; Sasbrink, Bart; Bag, Pushan; Blanken, Tristian den; Buijs, Ronald; Gracht, Lex van der; Hendriks, Frans; Lambrev, Petar; Limburg, Rob; Mascoli, Vincenzo; Nawrocki, Wojciech J; Reus, Michael; Parmessar, Ramon; Singerling, Björn; Stokkum, Ivo H M; Jansson, Stefan; Holzwarth, Alfred R.

doi:10.1111/ppl.14306

Cited by 2 publications

References 49 publications

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Quantification of electron transport-related oxidative signals by time and wavelength-resolved redox biosensors and chlorophyll fluorescence

Hipsch,

Lampl,

Lev

et al. 2024

Preprint

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Reductive and oxidative signals transmitted from the photosynthetic electron chain to target proteins through the redox signaling network are key regulators of carbon assimilation and downstream metabolism. However, despite their crucial role in activating and inhibiting photosynthetic activity, their relation to photosynthetic efficiency is hardly quantified due to the methodological gap between traditional spectroscopic approaches for investigating photosynthesis and biochemical analyses used in the redox regulation field. Here, we simultaneously quantified redox signals and photosynthetic activity by exploring time and wavelength-resolved fluorescence spectra that capture biosensor and chlorophyll fluorescence signals. Using a set of potato plants expressing genetically encoded redox biosensors capable of discerning between oxidized and reduced signals, we demonstrated how reductive and oxidative signals are amplified with elevated light intensities and revealed the tight connection between electron transport rate and the generation of oxidative signals. These results demonstrate how full spectrum analysis can pave the way for the integration of genetically encoded biosensors in photosynthesis research and demonstrate light-dependent activation of inhibitory oxidative signals in major crop plants.

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Quantification of electron transport-related oxidative signals by time and wavelength-resolved redox biosensors and chlorophyll fluorescence

Hipsch,

Lampl,

Lev

et al. 2024

Preprint

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From Photosynthesis to Industrial Applications

Funk,

Schröder

2024

Physiologia Plantarum

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Climate change, energy use and food security are the main challenges that our society faces nowadays. The impact of carbon dioxide on the earth's climate forces us to investigate the possibilities of using sustainable energy resources. One potential approach to address this problem is to recycle CO 2 for fuel or chemical production using photosynthesis. Photosynthetic organisms use solar energy to incorporate atmospheric CO 2 into organic molecules. Therefore, photosynthesis not only prevents climate change, but the produced biomass also provides food and feed, and even can be used in technical processes to gain valuable products and biofuels. While the principle is compelling, the practical integration of this concept still provides significant scientific and economic challenges.Algae and cyanobacteria are, without doubt, the most productive photosynthetic organisms on Earth. Their fast growth allows harvesting after a short time. Furthermore, the aquatic growth of microorganisms in tanks or ponds requires marginal land and allows a continuous, automated system with very high flow-through capacities. Even though microalgae are regarded as one of the most promising feedstocks for the production of biofuels, higher-value compounds, biofertilizers, food or feed, the energy costs for the production of algal biomass are still too high to be economic feasible. This special issue "From Photosynthesis to Industrial Applications", highlights the current research, developments and innovations performed on photosynthetic organisms (ranging from cyanobacteria to higher plants) to address our current knowledge gaps and challenges of the society. It is based on the conference with the same title, held in Umeå, Sweden, June 19-21, 2023, merging two congresses dealing with important questions for a sustainable future: the 16th Nordic Photosynthesis Congress (NPC) and the 5th Nordic Algae Symposia (NAS).

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ChloroSpec: A new in vivo chlorophyll fluorescence spectrometer for simultaneous wavelength‐ and time‐resolved detection

Cited by 2 publications

References 49 publications

Quantification of electron transport-related oxidative signals by time and wavelength-resolved redox biosensors and chlorophyll fluorescence

Quantification of electron transport-related oxidative signals by time and wavelength-resolved redox biosensors and chlorophyll fluorescence

From Photosynthesis to Industrial Applications

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