Multi-Color Quantum Dot Tracking Using a High-Speed Hyperspectral Line-Scanning Microscope

Cutler, Patrick J.; Malik, M. D.; Liu, Sheng; Byars, Jason M.; Lidke, Diane S.; Lidke, Keith A.

doi:10.1371/journal.pone.0064320

Cited by 91 publications

(97 citation statements)

References 52 publications

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“…In contrast to that, in our previous study, we found dimerization to be independent of receptor expression levels in the range from 20,000 to 260,000 copies per cell (19). According to single particle tracking studies of EGFR, the receptor forms transient dimers in the absence of EGF (20)(21)(22); on the other hand, results of Tao and Maruyama (23) indicate that EGFR dimers are already formed in the endoplasmic reticulum, which would suggest a much more stable association. Among possible explanations of the contradictory findings are the intrinsic differences between cell lines as well as differences in the state of the cells used in the individual studies.…”

Section: Introductioncontrasting

confidence: 99%

The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells

Yavas

Macháň

Wohland

2016

Biophysical Journal

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The epidermal growth factor receptor (EGFR) is a prototypical receptor tyrosine kinase involved in cell growth and proliferation and associated with various cancers. It is commonly assumed that after activation by binding of epidermal growth factor to the extracellular domain it dimerizes, followed by autophosphorylation of tyrosine residues at the intracellular domain. However, its oligomerization state before activation is controversial. In the absence of ligands, EGFR has been found in various, inconsistent amounts of monomeric, inactive dimeric, and oligomeric forms. In addition, evidence suggests that the active conformation is not a simple dimer but contains higher oligomers. As experiments in the past have been conducted at different conditions, we investigate here the influence of cell lines (HEK293, COS-7, and CHO-K1), temperature (room temperature and 37 C), and membrane localization on the quantitation of preformed dimers using SW-FCCS, DC-FCCS, quasi PIE-FCCS, and imaging FCCS. While measurement modality, temperature, and localization on upper or lower membranes have only a limited influence on the dimerization amount observed, the cell line and location to periphery versus center of the cell can change dimerization results significantly. The observed dimerization amount is strongly dependent on the expression level of endogenous EGFR in a cell line and shows a strong cell-to-cell variability even within the same cell line. In addition, using imaging FCCS, we find that dimers have a tendency to be found at the periphery of cells compared to central positions.

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Section: Introductioncontrasting

confidence: 99%

The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells

Yavas

Macháň

Wohland

2016

Biophysical Journal

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“…a nonconstant diffusion coefficient, which causes the nonergodic behavior. Recent measurements reporting on the variation of diffusion coefficient in space or time [10][11][12], sometimes associated with anomalous and nonergodic diffusion [7], justify these assumptions phenomenologically. However, some questions remain unanswered: What causes such kinetic heterogeneity at the microscopic level?…”

Section: Introductionmentioning

confidence: 98%

Nonergodic subdiffusion from transient interactions with heterogeneous partners

et al. 2017

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Spatiotemporal disorder has been recently associated to the occurrence of anomalous nonergodic diffusion of molecular components in biological systems, but the underlying microscopic mechanism is still unclear. We introduce a model in which a particle performs continuous Brownian motion with changes of diffusion coefficients induced by transient molecular interactions with diffusive binding partners. In spite of the exponential distribution of waiting times, the model shows subdiffusion and nonergodicity similar to the heavy-tailed continuous time random walk. The dependence of these properties on the density of binding partners is analyzed and discussed. Our work provides an experimentally-testable microscopic model to investigate the nature of nonergodicity in disordered media.

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“…Thus, the surface modification of QDs with the listed affinity tags and biomolecules (such as in proteins, peptides, DNAs carbohydrates, and drugs) allows the biohybrid to be employed in different biological applications, as shown in Figure 5 (Zrazhevskiy and Gao, 2013;Cutler et al, 2013).…”

Section: Semiconductor Quantum Dotsmentioning

confidence: 99%

Hybrid nanomaterial: biocolloidals

Gözübenli¹,

Yasun²,

Dilsiz³

2017

Turk J Biol

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Nanomaterials-based diagnostics have tremendous advantages over other conventional diagnostic systems in terms of sensitivity, specificity, and portability owing to the unique intrinsic properties of nanomaterials. Thus, there is a substantial need to develop and employ a broad spectrum of nanomaterials for biological and diagnostic applications. In this review, we focus on nanomaterials-assisted disease diagnosis utilizing different techniques such as photoluminescence, colorimetry, fluorescence, electrochemistry, microarray methods, surface plasmon resonance, and surface-enhanced Raman spectroscopy. In these techniques, different nanomaterials, including but not limited to gold and silver materials, metal oxides, and semiconductors, were employed according to their all-purpose chemical and physical properties. As the new properties of nanomaterials are discovered, their contributions to nanobiotechnology applications are vastly increased. In this review, the features of the selected nanobiomaterials, biological tag-modified nanomaterials, and their outstanding applications for the detection of specific biotargets have been summarized according to the latest studies. Nanomaterials contribute to the fields of photo/chemotherapy and biomedical imaging in particular, since all the biological events happen within this size range and beneficiary roles of nanoparticles (NPs) are countless in biomedical applications due to their unique physical and chemical properties. However, due to the necessity of specificity and selectivity, there is a clear ambition to study bioconjugation of NPs to increase the efficiency of the targeted biological applications. Thus, the combination of the specificity and the intrinsic properties of biohybrid NPs facilitate diagnostic and therapeutic applications.

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Multi-Color Quantum Dot Tracking Using a High-Speed Hyperspectral Line-Scanning Microscope

Cited by 91 publications

References 52 publications

The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells

The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells

Nonergodic subdiffusion from transient interactions with heterogeneous partners

Hybrid nanomaterial: biocolloidals

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