Multi-biomarker approach and IBR index to evaluate the effects of bisphenol A on embryonic stages of zebrafish (Danio rerio)

Heredia-García, Gerardo; Gómez-Oliván, Leobardo Manuel; Elizalde-Velázquez, Gustavo Axel; Cardoso-Vera, Jesús Daniel; Orozco-Hernández, José Manuel; Rosales-Pérez, Karina Elisa; García-Medina, Sandra; Islas-Flores, Hariz; Galár-Martínez, Marcela; Dublán‐García, Octavio

doi:10.1016/j.etap.2022.103925

Cited by 15 publications

(2 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, our data identified a number of cardiac and vasculature-related GO-terms induced by BPA exposure. This supports previously published BPA-mediated effects on heart development ( Achenbach et al 2020 ; Heredia-García et al 2022 ), vascular toxicity, and BPA-induced vascular development ( Ji et al 2022 ) in zebrafish. Similarly, previously reported issues regarding yolk and lipid defects including lipid uptake ( Mu et al 2022 ), metabolism ( Sun et al 2020 ), and lipid production ( Ortiz-Villanueva et al 2018 ) were also well-represented in the transcriptomic data.…”

Section: Discussionsupporting

confidence: 92%

“…Similarly, issues of pericardial edema ( Achenbach et al 2020 ) and decreased heart rate ( Sundarraj et al 2021 ) are well-documented. Mechanistically, BPA exposure has been shown to induce production of reactive oxygen species (ROS), leading to alterations in immune function ( Qian et al 2023 ), changes in brain morphology ( Yang et al 2023 ), and the expression of a number of developmental genes ( Heredia-García et al 2022 ). Although BPA-induced locomotor alterations have been described ( Wang et al 2021 ; Gu et al 2022 ), there is not yet a consensus on the precise source and extent of these deficits ( Coumailleau et al 2020 ; Scaramella et al 2022 ).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Comparative toxicological assessment of 2 bisphenols using a systems approach: evaluation of the behavioral and transcriptomic responses of Danio rerio to bisphenol A and tetrabromobisphenol A

Morash,

Kirzinger,

Achenbach

et al. 2024

Toxicological Sciences

View full text Add to dashboard Cite

The zebrafish (Danio rerio) is becoming a critical component of New Approach Methods (NAMs) in chemical risk assessment. As a whole organism in vitro NAM, the zebrafish model offers significant advantages over individual cell-line testing, including toxicokinetic and toxicodynamic competencies. A transcriptomic approach not only allows for insight into mechanism of action for both apical endpoints and unobservable adverse outcomes, but also changes in gene expression induced by lower, environmentally relevant concentrations. In this study, we used a larval zebrafish model to assess the behavioral and transcriptomic alterations caused by sub-phenotypic concentrations of two chemicals with the same structural backbone, the endocrine disrupting chemicals: Bisphenol A and Tetrabromobisphenol A. Following assessment of behavioral toxicity, we used a transcriptomic approach to identify molecular pathways associated with previously described phenotypes. We also determined the transcriptomic Point of Departure (POD) for each chemical by modelling gene expression changes as continuous systems which allows for the identification of a single concentration at which toxic effects can be predicted. This can then be investigated with confirmatory cell-based testing in an integrated approach to testing and assessment (IATA) to determine risk to human health and the environment with greater confidence. This paper demonstrates the impact of using a multi-faceted approach for evaluating the physiological and neurotoxic effects of exposure to structurally related chemicals. By comparing phenotypic effects with transcriptomic outcomes, we were able to differentiate, characterize and rank the toxicities of related bisphenols, which demonstrates methodological advantages unique to the larval zebrafish NAM.

show abstract