Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA

Dong, Xuan; Lin, Liang‐Hung; Chen, J.; Tian, Huayu; Xiao, Chunsheng; Guo, Zhaopei; Li, Y.; Wei, Yen; Chen, Xiaogang

doi:10.1016/j.actbio.2013.02.007

Cited by 16 publications

(22 citation statements)

References 40 publications

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“…PEI is an efficient non-viral nucleic acid carrier which is widely used in the research of cancer gene therapy [28]. As another common used transfection material, Poly (L-lysine) (PLL) could not condense DNA as effective as PEI, but prevailed because of its low toxicity in vivo [19].…”

Section: Discussionmentioning

confidence: 99%

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miRNA oligonucleotide and sponge for miRNA-21 inhibition mediated by PEI-PLL in breast cancer therapy

et al. 2015

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Section: Discussionmentioning

confidence: 99%

“…This would be attributed to the unstable structure of polymer/oligonucleotide complexes [28]. Next the zeta potentials of the complexes formed by polymers and pDNA or oligonucleotide were measured at the same transfection ratio (Fig.…”

Section: Discussionmentioning

confidence: 99%

miRNA oligonucleotide and sponge for miRNA-21 inhibition mediated by PEI-PLL in breast cancer therapy

et al. 2015

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“…The sizes and zeta potentials of the complexes of PEI‐MTU 35 ‐Gs and siRNA were measured by dynamic light scattering and a zeta potential analyzer at the different polymer/siRNA molar ratios ( M n , siRNA = 13 300 Da, Figure ). Compared with the PEI‐MTU 35 /pDNA complex with a range of 110–150 nm reported in our previous work, the PEI‐MTU 35 ‐G/siRNA complexes displayed a little larger sizes of about 140–180 nm at the same condition (Figure A), which could be attributed to a much smaller molecular weight of siRNA than that of pDNA . As mentioned previously, the guanidino group could form both electrostatic interaction and hydrogen bond with siRNA.…”

Section: Resultsmentioning

confidence: 50%

“…The sizes and surface charges of nanoparticles affect both cellular internalization efficiency and cytocompatibility . In general, the particles with tens to hundreds of nanometers and positive surface charges can be internalized by the cells more efficiently . The sizes and zeta potentials of the complexes of PEI‐MTU 35 ‐Gs and siRNA were measured by dynamic light scattering and a zeta potential analyzer at the different polymer/siRNA molar ratios ( M n , siRNA = 13 300 Da, Figure ).…”

Section: Resultsmentioning

confidence: 99%

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Guanidinated Thiourea‐Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery

Tian

Ding

et al. 2015

Adv Healthcare Materials

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RNA interference (RNAi) provides the promising treatments of gene-related diseases while hindered by the lack of highly efficient delivery platform with low cytotoxicity. Moreover, the intracellular fates of nonviral gene carriers are closely related to their internalization pathway, and eventually influence their RNAi efficiency. Herein, a series of guanidinated thiourea-modified polyethylenimines (PEI-MTU-Gs) are synthesized and utilized as the efficient carriers of small interfering RNA (siRNA) with up to 71.6% inhibition of luciferase activity in the luciferase-expressing cell lines (i.e., HeLa/Luc cells). The introduction of noncationic hydrogen bond donors, that is, thiourea groups, provides the carriers with much lower cytotoxicities and relatively looser complex structures that facilitate the intracellular release of siRNAs. Furthermore, the multiguanidino structures endow the PEI-MTU-G/siRNA complexes with the ability to directly penetrate cell membrane, which facilitates the cellular internalization while avoiding them suffering from the rigorous lysosomes. The results demonstrate PEI-MTU35 -Gs as promising siRNA carriers for further gene therapy.

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Oligo(ethylene imine)‐grafted glycidyl methacrylate linear and star homopolymers: Odd–even correlated transfection efficiency

Pafiti

Kepola

Vlasiou

et al. 2021

Journal of Polymer Science

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The present study aims to investigate an odd-even effect of the number of ethylene imine units in the side-groups of totally abiotic synthetic polymers on their efficiency in DNA transfection. A library of fifteen polymers was fabricated. Two star homopolymers and one linear homopolymer based on glycidyl methacrylate were synthesized and used as precursors to which five linear oligo(ethylene imine)s (OEI) were grafted. The number of ethylene imine groups of the OEIs was varied. Specifically, ethylene diamine, diethylene triamine, triethylene tetramine, tetraethylene pentamine, and pentaethylene hexamine were used. Each of these fifteen OEI-grafted polymers was evaluated in terms of their efficiency to transfer plasmid DNA encoding firefly luciferase in C2C12 mouse myoblast cells. The transfection efficiency displayed an odd-even pattern, with all OEI-grafted polymers with an odd number of ethylene imine repeating units exhibiting higher transfection efficiency compared with those possessing an even number of ethylene imine repeating units. The odd-even effect was more pronounced for the star polymers with longer arms (degree of polymerization, DP = 100), while in case of the linear polymers, the odd-even effect was only observed for the lowest polymer loading. The cytotoxicity of the OEI-grafted polymers also followed an odd-even pattern, with the OEI-grafted star polymers with an arm DP of 100 and the linear polymers clearly presenting an odd-even effect, while the cytotoxicity of the OEI-grafted star polymers with an arm DP of 20 slightly increased with the number of ethylene imine repeating units.

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Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA

Cited by 16 publications

References 40 publications

miRNA oligonucleotide and sponge for miRNA-21 inhibition mediated by PEI-PLL in breast cancer therapy

miRNA oligonucleotide and sponge for miRNA-21 inhibition mediated by PEI-PLL in breast cancer therapy

Guanidinated Thiourea‐Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery

Oligo(ethylene imine)‐grafted glycidyl methacrylate linear and star homopolymers: Odd–even correlated transfection efficiency

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