Mulberry leaves (Morus alba L.) ameliorate obesity-induced hepatic lipogenesis, fibrosis, and oxidative stress in high-fat diet-fed mice

Abstract

Obesity is associated with chronic diseases such as fatty liver, type 2 diabetes, cardiovascular disease, and severe metabolic syndrome. Obesity causes metabolic impairment including excessive lipid accumulation and fibrosis in the hepatic tissue as well as the increase in oxidative stress. In order to investigate the effect of mulberry leaf (Morus alba L.) extract (MLE) on obesity-induced oxidative stress, lipogenesis, and fibrosis in liver, MLE has been gavaged for 12 weeks in high-fat diet (HFD)-induced obese mice. MLE treatment significantly ameliorated LXRa-mediated lipogenesis and hepatic fibrosis markers such as a-smooth muscle actin, while MLE up-regulated lipolysis-associated markers such as lipoprotein lipase in the HFD-fed mice. Moreover, MLE normalized the activities of antioxidant enzymes including heme oxygenase-1 and glutathione peroxidase in accordance with protein levels of 4-hydroxynonenal in the HFDfed mice. MLE has beneficial effects on obesity-related fatty liver disease by regulation of hepatic lipid metabolism, fibrosis, and antioxidant defense system. MLE supplementation might be a potential therapeutic approach for obesity-related disease including non-alcoholic fatty liver disease.

Genetic diversity among mulberry genotypes from seven countries

“…Additionally, mulberry has both the anticancer and the anti-inflammatory properties, which act through different pathways (Kollar et al 2013;Eo et al 2014;Kwon et al 2015;Qian et al 2015). Some reports have demonstrated the leaf's role in ameliorating obesity-induced reactions, increasing bone density and antiplatelet activity (Ann et al 2015;Sungkamanee et al 2014;Kim et al 2014). Therefore, it is essential and beneficial for us to improve the production and quality of mulberry.…”

Therapeutic Impact of Berries (Morus Alba and Morus Rubra) Fruit Extract in the Regression of High-Fat Diet-Induced Cardiac Dysfunction in Rats

“…These findings may be explained by mulberry leaf extract (MLE) exhibited its useful effect on lipid profiles regulation and the atherogenic index, joined with reduction of fat accumulation in the liver [8]. Moreover, MLE decreased fibrosis of hepatic tissue as examined by collagen gene expressions.…”

“…It is rich in polyphenolic compounds including rutin, quercetin, and 1-deoxynojirimycin (DNJ). Hence, it has been medicated several diseases including dyslipidemia [7], diabetes [8], fatty liver [6], and hypertension diseases [8]. Besides, the mulberry fruits induce enzymatic antioxidant in diabetic models [8].…”

“…Hence, it has been medicated several diseases including dyslipidemia [7], diabetes [8], fatty liver [6], and hypertension diseases [8]. Besides, the mulberry fruits induce enzymatic antioxidant in diabetic models [8]. However, few studies pay particular attention to the mechanism in which fruits of the mulberry attenuate lipogenesis, lipolysis, and fibrosis in obese models induced by HFD.…”

Type 2 Diabetes and Developmental Origin of Non-Alcohol Fatty Liver Disease and Future Directions of Treatment

“…Quercetin-treatment has a great advantage of reducing AKT phosphorylation, and oxidative/nitrosative stress, inflammation and lipid metabolism-related genes which displayed affinity to normalize in both in vivo and in vitro models [157]. Other phytochemical plants such as Morus alba leaves extracts [158], black rice extract [159], barley beta-glucan [160] , pomegranate juice and peel [161,162], nuts rich in omega-3 fatty acids [163], resveratrol [164,165], green tea polyphenol Epigallocatechin-3-gallate [166], Fenugreek, Nigella, and termis seeds [167], Artemisia scoparia extract [168] and onion [169].…”