Mulberry leaf extract mediated synthesis of gold nanoparticles and its anti-bacterial activity against human pathogens

Abstract

Gold nanoparticles (Au-NPs) were synthesized at room temperature using Morus alba (mulberry) leaf extract as reducing and stabilizing agent. The development of plant mediated synthesis of nanoparticles is gaining importance due to its simplicity, low cost, non-toxicity, ecofriendliness, long term stability and reproducible aqueous synthesis method to obtain a selfassembly of nearly monodispersed Au-NPs. The formation and morphology of biosynthesized nanoparticles are investigated with the help of UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), atomic force microscopy (AFM), x-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR) techniques. Au-NPs formation was screened by UV-Vis spectroscopy through color conversion due to surface plasmon resonance band at 538 nm for Au-NPs. DLS studies revealed that the average size of Au-NPs was 50 nm. TEM studies showed the particles to be nearly spherical with few irregular shapes and particle size ranges 15−53 nm. The AFM image clearly shows the surface morphology of the well-dispersed Au-NPs with less than 50 nm. The high crystallinity of nanoparticles is evident from bright circular spots in the selected area electron diffraction (SAED) pattern. X-ray diffraction pattern showed high purity and face-centered cubic structure of Au-NPs. The FT-IR results indicate the presence of different functional groups present in the biomolecule capping the nanoparticles. Further, biosynthesized Au-NPs show strong zone of inhibition against Vibrio cholera (gram-negative) and Staphylococcus aureus (gram-positive) whereas, chemically synthesized Au-NPs and mulberry leaf extract exhibit a fair zone of inhibition.

Rapid Biosynthesis Method and Characterization of Silver Nanoparticles Using <i>Zizyphus spina christi</i> Leaf Extract and Their Antibacterial Efficacy in Therapeutic Application

“…Although biosynthesis of nanoparticles using plant extracts has already been reported in various plants such as neem [11], Aloe vera [12], Cinnamomum camphora [13], Rosa hybride [14] and Mulberry leaf [15], there is still a lot of attention to this field because of the diversity and the high potential of plants as reducing agent in producing nanoparticles with different shapes. There are many solvents used for extracting the plant components for nanoparticles synthesis like methanol [15] ethanol [16], diethyl ether, chloroform, ethyl acetate [17] and water [18] [19] [20].…”

“…There are many solvents used for extracting the plant components for nanoparticles synthesis like methanol [15] ethanol [16], diethyl ether, chloroform, ethyl acetate [17] and water [18] [19] [20]. As the research is focused on eco-friendly and low cost methods for synthesis of nanoparticles, it is always preferable to use water (aqueous solvent) for extraction procedures and preventing the use of toxic chemicals.…”

Phytochemical Synthesis and Characterization of Bioactive Gold Nanoparticles using Hypaphorine and their Cytotoxic Activity against HCT 116 Human Colorectal Cancer Cells

“…The obtained spectral data was then recorded and plotted using Origin Pro software version 8.1. FTIR FTIR spectroscopy is used to identify the functional groups that are bound to the surface of NPs [3,26]. The identification of functional groups are important due to the fact that the surface chemistry of AuNPs affects their properties and applications [8].…”

“…There is a significant interest towards the use of biologically synthesised and medical applications of metallic nanoparticles as they provide completely new and improved properties which are based on size and shape [2,3]. Amongst metallic nanoparticles, AuNPs possesses remarkably unique physicochemical properties, tunable optical properties, good Manuscript received August 26, 2016.…”

“…Many researchers have recently reported the biosynthesis of AuNPs using phytochemicals, which have become an interesting area of research in the biomedical field by producing safe nanoparticles that can be used in medical applications. A plethora of research studies in which AuNPs are synthesized using plant extracts and pure phytochemicals have been reported, with a few of them being mulberry leaf extract [3], Achyranthes asparalin seed extract [10], Solanum nigrum leaf extract [5], Ananas comosus fruit extract [11], Elettaria cardamomum aqueous extract [12], Stevia rebaudiana leaf extract [13], Buta monosperma leaf extract [14], gallic acid [15] Epigallocatechin-3-gallate (EGCG) [16] and Hesperidin [17]. Although there are many reports, the fabrication methods of AuNPs using phytochemicals and their application in cancer therapy are still sparse.…”