Mueller-matrix of laser-induced autofluorescence of polycrystalline films of dried peritoneal fluid in diagnostics of endometriosis

Abstract

This research presents investigation results of the diagnostic efficiency of an azimuthally stable Mueller-matrix method of analysis of laser autofluorescence of polycrystalline films of dried uterine cavity peritoneal fluid. A model of the generalized optical anisotropy of films of dried peritoneal fluid is proposed in order to define the processes of laser autofluorescence. The influence of complex mechanisms of both phase (linear and circular birefringence) and amplitude (linear and circular dichroism) anisotropies is taken into consideration. The interconnections between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy are determined. The statistical analysis of coordinate distributions of such Mueller-matrix rotation invariants is proposed. Thereupon the quantitative criteria (statistic moments of the first to the fourth order) of differentiation of polycrystalline films of dried peritoneal fluid, group 1 (healthy donors) and group 2 (uterus endometriosis patients), are determined.

Mueller matrix mapping of biological polycrystalline layers using reference wave

“…Here: • LD and LB -linear dichroism and birefringence for the direction of the optical axis light amplitude; Such a parameters are in particular investigated for optically thin (non-depolarizing) layers of the histological sections of biological tissues and films of biological liquids [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] . For depolarizing layers such data is not obtained.…”

Jones matrix mapping of polycrystalline networks of layers of main types of amino acids

“…The actual direction in laser polarimetric diagnostics [1][2][3][4][5][6][7][8][9][10][11] of biological objects is the development of matrix methods [12][13][14][15], which provide the most complete information about the polycrystalline structure [16][17][18][19][20][21] of films of biological fluids of human organs. We study possibilities of the development and experimental testing of the Jones matrix (JM) mapping method for polycrystalline layers of basic amino acids of the human body in order to obtain parameters for the differential diagnosis of pathological conditions.…”

2D-Mueller-matrix tomography of optically anisotropic polycrystalline networks of biological tissues histological sections

“…Here, based on the statistical approach, correlations are found between the average, dispersion, skewness, and kurtosis, which characterize the phase-inhomogeneous layers and Mueller-matrix elements of the distributions [13][14][15]. On this basis, diagnostic criteria for cancer and other pathologies of human organs were found [16][17][18][19]. However, real biological media usually have depolarizing effect on the laser beam, propagating in them [20][21][22][23].…”