Mucosal immunisation with novel<i>Streptococcus pneumoniae</i>protein antigens enhances bacterial clearance in an acute mouse lung infection model

Jomaa, Maha; Kyd, Jennelle M.; Cripps, Allan W.

doi:10.1016/j.femsim.2004.12.001

Cited by 15 publications

(11 citation statements)

References 41 publications

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“…It has also been reported that GADPH is an immunogenic protein found on the cell wall of SS [15]. GAPDH is reported in the development of subunit vaccines against Edwardsiella tarda [31], [32], Streptococcus pneumoniae [33] and Bacillus anthracis [34].…”

Section: Discussionmentioning

confidence: 96%

Comparative Proteomic Analysis of Streptococcus suis Biofilms and Planktonic Cells That Identified Biofilm Infection-Related Immunogenic Proteins

Wang

et al. 2012

PLoS ONE

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Streptococcus suis (SS) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. Biofilms of SS bind to extracellular matrix proteins in both endothelial and epithelial cells and cause persistent infections. In this study, the differences in the protein expression profiles of SS grown either as planktonic cells or biofilms were identified using comparative proteomic analysis. The results revealed the existence of 13 proteins of varying amounts, among which six were upregulated and seven were downregulated in the Streptococcus biofilm compared with the planktonic controls. The convalescent serum from mini-pig, challenged with SS, was applied in a Western blot assay to visualize all proteins from the biofilm that were grown in vitro and separated by two-dimensional gel electrophoresis. A total of 10 immunoreactive protein spots corresponding to nine unique proteins were identified by MALDI-TOF/TOF-MS. Of these nine proteins, five (Manganese-dependent superoxide dismutase, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, ornithine carbamoyltransferase, phosphoglycerate kinase, Hypothetical protein SSU05_0403) had no previously reported immunogenic properties in SS to our knowledge. The remaining four immunogenic proteins (glyceraldehyde-3-phosphate dehydrogenase, hemolysin, pyruvate dehydrogenase and DnaK) were identified under both planktonic and biofilm growth conditions. In conclusion, the protein expression pattern of SS, grown as biofilm, was different from the SS grown as planktonic cells. These five immunogenic proteins that were specific to SS biofilm cells may potentially be targeted as vaccine candidates to protect against SS biofilm infections. The four proteins common to both biofilm and planktonic cells can be targeted as vaccine candidates to protect against both biofilm and acute infections.

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Section: Discussionmentioning

confidence: 96%

Comparative Proteomic Analysis of Streptococcus suis Biofilms and Planktonic Cells That Identified Biofilm Infection-Related Immunogenic Proteins

Wang

et al. 2012

PLoS ONE

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“…In this study, we focused on testing the S. pneumoniae-derived RP-L7/L12 protein, which we argue is a more appropriate target for detecting S. pneumoniae in the following ways. First, RP-L7/L12 is a component of the 50S ribosome, which is abundant in all bacteria and is specific for each bacterial species (26,34). Second, the RP-L7/L12 levels increase in proportion to the bacterial growth rate, since the protein is essential for protein synthesis in bacteria (25,35).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Usefulness of Ribosomal Protein L7/L12 for Pneumococcal Pneumonia in a Mouse Model

et al. 2013

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The capsular antigen detection (CAD) kit is widely used in clinics to detect Streptococcus pneumoniae infection from urine, because it is rapid, convenient, and effective. However, there are several disadvantages, including false-positive results in children colonized with S. pneumoniae and prolonged positive readings even after the bacteria have been cleared. RP-L7/L12 is a component of the 50S ribosome that is abundant in all bacteria and is specific for each bacterial species. We investigated whether RP-L7/L12 could be used to accurately diagnose pneumococcal pneumonia infection in mouse models of pneumonia and colonization generated by infecting CBA/JN or CBA/N mice, respectively, with S. pneumoniae strain 741. RP-L7/L12 detection by enzyme-linked immunosorbent assay accurately assessed active lung infection, as RP-L7/L12 levels decreased simultaneously with the bacterial lung burden after imipenem administration in the pneumonia mouse model. Based on the data, antibodies detecting RP-L7/L12 were applied to rapid immunochromatographic strips (ICS) for urine sample testing. When we compared the ICS test with the CAD kit in the pneumonia model, the results correlated well. Interestingly, however, when the lung bacterial burden became undetectable after antibiotic treatment, the ICS test was correspondingly negative, even though the same samples tested by the CAD kit remained positive. Similarly, while the ICS test exhibited negative results in the nasal colonization model, the CAD kit demonstrated positive results. Bacterial RP-L7/L12 may be a promising target for the development of new methods to diagnose infectious disease. Further studies are warranted to determine whether such a test could be useful in children.

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“…This recruitment into the lung is in response to bacterial lipopolysacharide and a range of inflammatory mediators that are released by resident phagocytes and endothelial cells of the bronchus (Dunkley et al, 1995). Immunization with antigens from NTHi, Streptococcus pneumoniae and Pseudomonas aeruginosa have previously been reported to enhance the recruitment of white cells (both neutrophils and monocytes) when the lung is challenged with live microbes (Buret et al, 1994; Foxwell et al, 2001; Jomaa et al, 2005b). Furthermore, this recruitment is antigen dependent with immunization by WKCs normally be the most effective (Jomaa et al, 2005b).…”

Section: Discussionmentioning

confidence: 99%

Mucosal Immunization with the Moraxella Catarrhalis Porin M35 Induces Enhanced Bacterial Clearance from the Lung: A Possible Role for Opsonophagocytosis

Easton¹,

Cripps²,

Foxwell³

et al. 2011

Front. Immun.

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Moraxella catarrhalis is a significant cause of respiratory tract infection against which a vaccine is sought. Several outer membrane proteins are currently under investigation as potential vaccine antigens, including the porin M35. We have previously shown that the third external loop of M35 was immunodominant over the remainder of the protein for antibody produced in mice against the refolded recombinant protein. However, as this loop is predicted to fold inside the porin channel we also predicted that it would not be accessible to these antibodies when M35 is expressed on the surface of the bacteria in its native conformation. This study investigated the functional activity of antibodies against M35 and those specific for the loop 3 region of M35 in vitro and in vivo. Antisera from mice immunized with M35 or the loop 3-deletion, M35loop3−, recombinant proteins were not bactericidal but did have enhanced opsonic activity, whereas antibodies raised against the loop 3 peptide were not opsoniszing indicating that the immunodominant loop 3 of M35 was not accessible to antibody as we had previously predicted. Mucosal immunization with M35, M35 that had an antigenically altered loop 3 [M35(ID78)] and M35loop3− enhanced the clearance of M. catarrhalis from the lungs of mice challenged with live M. catarrhalis. The in vivo clearance of bacteria in the mice with the M35-derived protein constructs correlated significantly (p < 0.001) with the opsonic activity assessed an in vitro opsonophagocytosis assay. This study has demonstrated that the immunodominant B-cell epitope to loop 3 of the M. catarrhalis outer membrane protein M35 is not associated with immune protection and that M35-specific antibodies are not bactericidal but are opsoniszing. The opsoniszing activity correlated with in vivo clearance of the bacteria suggesting that opsoniszing antibody may be a good correlate of immune protection.

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Mucosal immunisation with novelStreptococcus pneumoniaeprotein antigens enhances bacterial clearance in an acute mouse lung infection model

Cited by 15 publications

References 41 publications

Comparative Proteomic Analysis of Streptococcus suis Biofilms and Planktonic Cells That Identified Biofilm Infection-Related Immunogenic Proteins

Comparative Proteomic Analysis of Streptococcus suis Biofilms and Planktonic Cells That Identified Biofilm Infection-Related Immunogenic Proteins

Diagnostic Usefulness of Ribosomal Protein L7/L12 for Pneumococcal Pneumonia in a Mouse Model

Mucosal Immunization with the Moraxella Catarrhalis Porin M35 Induces Enhanced Bacterial Clearance from the Lung: A Possible Role for Opsonophagocytosis

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