Comparative Proteomic Analysis of Streptococcus suis Biofilms and Planktonic Cells That Identified Biofilm Infection-Related Immunogenic Proteins

Abstract

Streptococcus suis (SS) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. Biofilms of SS bind to extracellular matrix proteins in both endothelial and epithelial cells and cause persistent infections. In this study, the differences in the protein expression profiles of SS grown either as planktonic cells or biofilms were identified using comparative proteomic analysis. The results revealed the existence of 13 proteins of varying amounts, among which six were upregulated and seven were downregulated in the Streptococcus biofilm compared with the planktonic controls. The convalescent serum from mini-pig, challenged with SS, was applied in a Western blot assay to visualize all proteins from the biofilm that were grown in vitro and separated by two-dimensional gel electrophoresis. A total of 10 immunoreactive protein spots corresponding to nine unique proteins were identified by MALDI-TOF/TOF-MS. Of these nine proteins, five (Manganese-dependent superoxide dismutase, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, ornithine carbamoyltransferase, phosphoglycerate kinase, Hypothetical protein SSU05_0403) had no previously reported immunogenic properties in SS to our knowledge. The remaining four immunogenic proteins (glyceraldehyde-3-phosphate dehydrogenase, hemolysin, pyruvate dehydrogenase and DnaK) were identified under both planktonic and biofilm growth conditions. In conclusion, the protein expression pattern of SS, grown as biofilm, was different from the SS grown as planktonic cells. These five immunogenic proteins that were specific to SS biofilm cells may potentially be targeted as vaccine candidates to protect against SS biofilm infections. The four proteins common to both biofilm and planktonic cells can be targeted as vaccine candidates to protect against both biofilm and acute infections.

Coprinuslactone protects the edible mushroom Coprinus comatus against biofilm infections by blocking both quorum‐sensing and MurA

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“…23−28 By studying biofilm formation in C. neoformans , we hope to identify general features and specific characteristics of biofilms.…”

Comparative proteomic and genomic analyses of Brucella abortus biofilm and planktonic cells

“…Protein was precipitated as previously described (10,20). The biofilm and planktonic bacteria were harvested by centrifugation (6,000 x g) at 4˚C for 10 min.…”

“…Biofilm formation is a critical survival mechanism for bacteria in the environment (8). altered gene and protein expression in biofilms is responsible for cell virulence, adherence and drug resistance (9,10). additionally, biofilm-grown microorganisms have an inherent lack of susceptibility to antibiotics (11)(12)(13).…”