Mucosa-associated microbiota signature in colorectal cancer

Gao, Renyuan; Kong, Cheng; Huang, Lyen C.; Li, H.; Qu, Xiao; Liu, Z.; Lan, Ping; Wang, J.; Qin, Huanlong

doi:10.1007/s10096-017-3026-4

Cited by 92 publications

(95 citation statements)

References 73 publications

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“…Some of the tumour genera of (possible) oral origin identi ed in our study, were associated with tumour mucosa previously, namely Fusobacterium, Campylobacter, Leptotrichia, Parvimonas, Gemella, Granulicatella, Eikenella, Selenomonas, Neisseria, Streptococcus, Alloprevotella, Veilonella, Haemophilus [8,31,32,[61][62][63][64][65][66][67][68]. In contrast, Solobacterium was reported increased [31], while Slackia and Pseudomonas were shown to be decreased [12,19] in fecal samples of CRC patients compared to healthy donors, and, in one epidemiological study, the presence of Treponema in the oral cavity was associated with increased risk of CRC [29], but none of these were previously associated with tumour mucosa.…”

Section: Tumour Crc Microbial Subtypessupporting

confidence: 57%

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Zwinsová¹,

Петров²,

Hrivňáková³

et al. 2020

Preprint

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BackgroundLong-term dysbiosis of the gut microbiome has a significant impact on the development, progression and the aggressiveness of colorectal cancer (CRC) and may explain part of the observed heterogeneity of the disease from phenotypic, prognostic and response to treatment perspectives. Although the shifts in gut microbiome in the normal-adenoma-carcinoma sequence have been described, the landscape of microbiome within CRC and its associations with clinical variables remain under-explored. ResultsWe performed 16S rRNA gene sequencing of paired tumour tissue, adjacent visually-normal mucosa and stool swabs of N=186 patients with stage 0-IV CRC to describe the tumour microbiome and its association with clinical variable and to derive tumour microbial subtypes.We identified new genera never previously associated with CRC tumour mucosa (Flavonifractor, Haemophilus, Howardella, Pseudomonas, Sutterella, Treponema 2) or CRC (Actinobacillus, Aggregatibacter, Bergeyella, Phocaeiola, Defluviitaleaceae UCG-011, Massilia, Tyzzerella 4). The bacteria residing on tumour-mucosa were dominated by genera belonging to (potential) oral pathogens. Based on tumour microbial profiles, we stratified CRC patients into three subtypes. The subtypes were significantly associated with prognostic factors such as tumor grade, primary tumour sidedness and TNM staging, with one subtype enriched in tumours with poor prognosis. Further, we inspected the associations of microbiome with clinical variables in a subtype-agnostic setting. The primary tumour-associated clinical variables predominantly correlated with tumour mucosal microbiome, while the presence of local and distant metastases was mostly associated with the stool microbiome.ConclusionsUnderstanding the interactions of the bacteria residing on tumour mucosa within different CRC tumour microbiome subtypes will help to better understand the underlying biological background of the heterogeneity of this disease. Indeed, the tumour microbiome is a possible source of additional integrative markers of CRC patients’ survival and prognosis. We found that CRC microbiome is strongly correlated with clinical variables, but these associations are dependent on the microbial environment (tumour mucosa, normal mucosa, stool). Our study thus identifies limitations of the usage of microbiome composition as marker of CRC progression, suggesting the need of combining several sampling sites (e.g. stool and tumour swabs).

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Section: Tumour Crc Microbial Subtypessupporting

confidence: 57%

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Zwinsová¹,

Петров²,

Hrivňáková³

et al. 2020

Preprint

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“…The Prevotella, Peptostreptococcus, and other opportunistic bacteria were enriched at the cancer sites. 20,21 Previous studies have investigated that the presence of fecal Bacteroidaceae, Fusobacteriaceae (i.e., Fusobacterium), Peptostreptococcaceae and Porphyromonas was relatively abundant in CRC patients. 22 Hypothetically, tumor tissues and adjacent normal tissues exhibit a better correlation A B C Figure 9 Diversity comparison of microbiota assemblage among CRC patients with S1, S2, and S3 staging.…”

Section: Discussionmentioning

confidence: 99%

<p>Comparison of Gut Microbiome in Human Colorectal Cancer in Paired Tumor and Adjacent Normal Tissues</p>

Sheng

et al. 2020

OTT

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Background: To understand the biological effect of gut microbiome on the progression of colorectal cancer (CRC), we sequenced the V3-V4 region of the 16S rRNA gene to illustrate the overall structure of microbiota in the CRC patients. Methods: In this study, a total of 66 CRC patients were dichotomized into different groups based on the following characteristics: paired tumor and adjacent normal tissues, distal and proximal CRC segments, MMR (-) and MMR (+), different TNM staging and clinic tumor staging. Results: By sequencing and comparing the microbial assemblages, our results indicated that 7 microbe genus (Fusobacterium, Faecalibacterium, Akkermansia, Ruminococcus2, Parabacteroides, Streptococcus, and f_Ruminococcaceae) were significantly different between tumor and adjacent normal tissues; and 5 microbe genus (Bacteroides, Fusobacterium, Faecalibacterium, Parabacteroides, and Ruminococcus2) were significantly different between distal and proximal CRC segments; only 2 microbe genus (f_Enterobacteriaceae and Granulicatella) were significantly different between MMR (-) and MMR (+); but there was no significant microbial difference were detected neither in the TNM staging nor in the clinic tumor staging. Conclusion: All these findings implied a better understanding of the alteration in the gut microbiome, which may offer new insight into diagnosing and therapying for CRC patients.

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“…Many studies show that Fusobacterium is more abundant in the intestines of CRC patients compared to the healthy individuals [32,33,36,38,42,45,46]. Comparation between CRC tissues and adjacent noncancer tissues also reveals that Fusobacterium is more abundant in the CRC tissues (Table 2) [ 30,36,37,43,44]. These studies indicate that there is a close relationship between Fusobacterium and CRC development.…”

Section: Porphyromonasmentioning

confidence: 93%

“…It is enriched in CRC and oral cancer patients, with its abundance in the intestines of CRC patients being higher than in healthy individuals [33,45,46]. In the CRC patients, Parvimonas exhibits higher abundance in CRC tissues compared to adjacent non-cancer tissues (Table 2) [30,44].…”

Section: Parvimonasmentioning

confidence: 99%

“…Peptostreptococcus is considered to be a carcinogenic bacterium that promotes the development of CRC, and many studies have found that the abundance of Peptostreptococcus in the intestines of CRC patients is higher than that of healthy individuals [32,33,35,36,42,46]. In CRC patients, Peptostreptococcus also exhibits higher abundance in CRC tissues compared to adjacent non-cancer tissues (Table 2) [30,44].…”

Section: Peptostreptococcusmentioning

confidence: 99%

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Foes or Friends? Bacteria Enriched in the Tumor Microenvironment of Colorectal Cancer

Yin

Zhang

et al. 2020

Cancers

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Colorectal cancer (CRC) is the second most commonly diagnosed cancer and the third cause of cancer death in the world, while intestinal microbiota is a community of microbes living in human intestine that can potentially impact human health in many ways. Accumulating evidence suggests that intestinal microbiota, especially that from the intestinal bacteria, play a key role in the CRC development; therefore, identification of bacteria involved in CRC development can provide new targets for the CRC diagnosis, prevention, and treatment. Over the past decade, there have been considerable advances in applying 16S rDNA sequencing data to verify associated intestinal bacteria in CRC patients; however, due to variations of individual and environment factors, these results seem to be inconsistent. In this review, we scrutinized the previous 16S rDNA sequencing data of intestinal bacteria from CRC patients, and identified twelve genera that are specifically enriched in the tumor microenvironment. We have focused on their relationship with the CRC development, and shown that some bacteria could promote CRC development, acting as foes, while others could inhibit CRC development, serving as friends, for human health. Finally, we highlighted their potential applications for the CRC diagnosis, prevention, and treatment.

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Mucosa-associated microbiota signature in colorectal cancer

Cited by 92 publications

References 73 publications

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

<p>Comparison of Gut Microbiome in Human Colorectal Cancer in Paired Tumor and Adjacent Normal Tissues</p>

Foes or Friends? Bacteria Enriched in the Tumor Microenvironment of Colorectal Cancer

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